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Literature DB >> 2764567

Hybridization of DNA probes with whole-community genome for detection of genes that encode microbial responses to pollutants: mer genes and Hg2+ resistance.

Abstract

Nucleic acids extracted from microbial biomass without prior culturing were hybridized with probes representing four mer operons to detect genes encoding adaptation to Hg2+ in whole-community genomes. A 29-fold enrichment in sequences similar to the mer genes of transposon Tn501 occurred during adaptation in a freshwater community. In an estuarine community, all four mer genes were only slightly enriched (by three- to fivefold), suggesting that additional, yet uncharacterized, mer genes encoded adaptation to Hg2+.

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Year: 1989 PMID： 2764567 PMCID： PMC202907 DOI： 10.1128/aem.55.6.1574-1577.1989

Source DB: PubMed Journal: Appl Environ Microbiol ISSN： 0099-2240 Impact factor: 4.792

10 in total

1. Cloning and expression in Escherichia coli of chromosomal mercury resistance genes from a Bacillus sp.

Authors: Y Wang; I Mahler; H S Levinson; H O Halvorson
Journal: J Bacteriol Date: 1987-10 Impact factor: 3.490

2. Adaptation of aquatic microbial communities to hg stress.

Authors: T Barkay
Journal: Appl Environ Microbiol Date: 1987-12 Impact factor: 4.792

3. Environmental significance of the potential for mer(Tn21)-mediated reduction of Hg2+ to Hg0 in natural waters.

Authors: T Barkay; C Liebert; M Gillman
Journal: Appl Environ Microbiol Date: 1989-05 Impact factor: 4.792

Review 4. Plasmid-mediated heavy metal resistances.

Authors: S Silver; T K Misra
Journal: Annu Rev Microbiol Date: 1988 Impact factor: 15.500

5. A procedure for the preparation of RNA-free plasmid DNA.

Authors: C Sadhu; L Gedamu
Journal: Biotechniques Date: 1988-01 Impact factor: 1.993

6. The distribution and divergence of DNA sequences related to the Tn21 and Tn501 mer operons.

Authors: M P Gilbert; A O Summers
Journal: Plasmid Date: 1988-09 Impact factor: 3.466

7. Preparation of a DNA gene probe for detection of mercury resistance genes in gram-negative bacterial communities.

Authors: T Barkay; D L Fouts; B H Olson
Journal: Appl Environ Microbiol Date: 1985-03 Impact factor: 4.792

8. Genetic and molecular characterization of the Pseudomonas plasmid pVS1.

Authors: Y Itoh; J M Watson; D Haas; T Leisinger
Journal: Plasmid Date: 1984-05 Impact factor: 3.466

9. Resistance to mercury and to cadmium in chromosomally resistant Staphylococcus aureus.

Authors: W Witte; L Green; T K Misra; S Silver
Journal: Antimicrob Agents Chemother Date: 1986-04 Impact factor: 5.191

10. Use of phylogenetically based hybridization probes for studies of ruminal microbial ecology.

Authors: D A Stahl; B Flesher; H R Mansfield; L Montgomery
Journal: Appl Environ Microbiol Date: 1988-05 Impact factor: 4.792

10 in total

31 in total

1. Terminal restriction fragment length polymorphism monitoring of genes amplified directly from bacterial communities in soils and sediments.

Authors: K D Bruce; M R Hughes
Journal: Mol Biotechnol Date: 2000-11 Impact factor: 2.695

2. Sequencing bands of ribosomal intergenic spacer analysis fingerprints for characterization and microscale distribution of soil bacterium populations responding to mercury spiking.

Authors: L Ranjard; E Brothier; S Nazaret
Journal: Appl Environ Microbiol Date: 2000-12 Impact factor: 4.792

10. The relationships of Hg(II) volatilization from a freshwater pond to the abundance ofmer genes in the gene pool of the indigenous microbial community.

Authors: T Barkay; R R Turner; A Vandenbrook; C Liebert
Journal: Microb Ecol Date: 1991-12 Impact factor: 4.552

Hybridization of DNA probes with whole-community genome for detection of genes that encode microbial responses to pollutants: mer genes and Hg2+ resistance.

1. Cloning and expression in Escherichia coli of chromosomal mercury resistance genes from a Bacillus sp.

2. Adaptation of aquatic microbial communities to hg stress.

3. Environmental significance of the potential for mer(Tn21)-mediated reduction of Hg2+ to Hg0 in natural waters.

Review 4. Plasmid-mediated heavy metal resistances.

5. A procedure for the preparation of RNA-free plasmid DNA.

6. The distribution and divergence of DNA sequences related to the Tn21 and Tn501 mer operons.

7. Preparation of a DNA gene probe for detection of mercury resistance genes in gram-negative bacterial communities.

8. Genetic and molecular characterization of the Pseudomonas plasmid pVS1.

9. Resistance to mercury and to cadmium in chromosomally resistant Staphylococcus aureus.

10. Use of phylogenetically based hybridization probes for studies of ruminal microbial ecology.

1. Terminal restriction fragment length polymorphism monitoring of genes amplified directly from bacterial communities in soils and sediments.

2. Sequencing bands of ribosomal intergenic spacer analysis fingerprints for characterization and microscale distribution of soil bacterium populations responding to mercury spiking.

3. Amplification of DNA from native populations of soil bacteria by using the polymerase chain reaction.

Review 4. Molecular mechanisms of genetic adaptation to xenobiotic compounds.

5. Identification of distinct communities of sulfate-reducing bacteria in oil fields by reverse sample genome probing.

6. Rapid method for direct extraction of mRNA from seeded soils.

7. Potential for mercury reduction by microbes in the high arctic.

8. Stable carbon isotope analysis of nucleic acids to trace sources of dissolved substrates used by estuarine bacteria.

9. Acclimation of aquatic microbial communities to Hg(II) and CH3Hg (+) in polluted freshwater ponds.

10. The relationships of Hg(II) volatilization from a freshwater pond to the abundance ofmer genes in the gene pool of the indigenous microbial community.