Literature DB >> 2820946

Cloning and expression in Escherichia coli of chromosomal mercury resistance genes from a Bacillus sp.

Y Wang1, I Mahler, H S Levinson, H O Halvorson.   

Abstract

A 7.9-kilobase (kb) chromosomal fragment was cloned from a mercury-resistant Bacillus sp. In Escherichia coli, in the presence of a second plasmid carrying functional transport genes, resistance to HgCl2 and to phenylmercury acetate (PMA) was expressed. Shortening the cloned fragment to 3.8 kb abolished resistance to PMA but not to HgCl2. In Bacillus subtilis, the 3.8-kb fragment produced mercuric reductase constitutively but did not produce resistance to HgCl2 or to PMA.

Entities:  

Mesh：

Substances：

Year:  1987        PMID： 2820946      PMCID： PMC213867          DOI： 10.1128/jb.169.10.4848-4851.1987

Source DB:  PubMed          Journal:  J Bacteriol        ISSN： 0021-9193            Impact factor:   3.490


  25 in total

1.  Detection of specific sequences among DNA fragments separated by gel electrophoresis.

Authors:  E M Southern
Journal:  J Mol Biol       Date:  1975-11-05       Impact factor: 5.469

2.  Labeling deoxyribonucleic acid to high specific activity in vitro by nick translation with DNA polymerase I.

Authors:  P W Rigby; M Dieckmann; C Rhodes; P Berg
Journal:  J Mol Biol       Date:  1977-06-15       Impact factor: 5.469

3.  A rapid alkaline extraction procedure for screening recombinant plasmid DNA.

Authors:  H C Birnboim; J Doly
Journal:  Nucleic Acids Res       Date:  1979-11-24       Impact factor: 16.971

4.  Revised interpretation of the origin of the pSC101 plasmid.

Authors:  S N Cohen; A C Chang
Journal:  J Bacteriol       Date:  1977-11       Impact factor: 3.490

5.  Penicillinase plasmids of Staphylococcus aureus: restriction-deletion maps.

Authors:  R P Novick; E Murphy; T J Gryczan; E Baron; I Edelman
Journal:  Plasmid       Date:  1979-01       Impact factor: 3.466

6.  Isolation and characterization of four plasmids from Bacillus subtilis.

Authors:  T Tanaka; M Kuroda; K Sakaguchi
Journal:  J Bacteriol       Date:  1977-03       Impact factor: 3.490

7.  Mercury and organomercurial resistances determined by plasmids in Staphylococcus aureus.

Authors:  A A Weiss; S D Murphy; S Silver
Journal:  J Bacteriol       Date:  1977-10       Impact factor: 3.490

8.  Heavy-metal and antibiotic resistance in the bacterial flora of sediments of New York Bight.

Authors:  J F Timoney; J Port; J Giles; J Spanier
Journal:  Appl Environ Microbiol       Date:  1978-09       Impact factor: 4.792

9.  Development of competence in the Bacillus subtilis transformation system.

Authors:  K F Bott; G A Wilson
Journal:  J Bacteriol       Date:  1967-09       Impact factor: 3.490

10.  Deletions in the r-determinant mer region of plasmid R100-1 selected for loss of mercury hypersensitivy.

Authors:  T J Foster; H Nakahara
Journal:  J Bacteriol       Date:  1979-10       Impact factor: 3.490
View more
  16 in total

1.  Cell-free mercury volatilization activity from three marine caulobacter strains.

Authors:  G Y Ji; S P Salzberg; S Silver
Journal:  Appl Environ Microbiol       Date:  1989-02       Impact factor: 4.792

2.  Distribution of DNA Sequences Encoding Narrow- and Broad-Spectrum Mercury Resistance.

Authors:  Paul A Rochelle; Mary K Wetherbee; Betty H Olson
Journal:  Appl Environ Microbiol       Date:  1991-06       Impact factor: 4.792

3.  Genes encoding mercuric reductases from selected gram-negative aquatic bacteria have a low degree of homology with merA of transposon Tn501.

Authors:  T Barkay; M Gillman; C Liebert
Journal:  Appl Environ Microbiol       Date:  1990-06       Impact factor: 4.792

4.  Phylogeny of mercury resistance (mer) operons of gram-negative bacteria isolated from the fecal flora of primates.

Authors:  C A Liebert; J Wireman; T Smith; A O Summers
Journal:  Appl Environ Microbiol       Date:  1997-03       Impact factor: 4.792

5.  Hybridization of DNA probes with whole-community genome for detection of genes that encode microbial responses to pollutants: mer genes and Hg2+ resistance.

Authors:  T Barkay; C Liebert; M Gillman
Journal:  Appl Environ Microbiol       Date:  1989-06       Impact factor: 4.792

6.  Mercury resistance in Bacillus cereus RC607: transcriptional organization and two new open reading frames.

Authors:  A Gupta; L T Phung; L Chakravarty; S Silver
Journal:  J Bacteriol       Date:  1999-11       Impact factor: 3.490

7.  Plasmid mediated metal and antibiotic resistance in marine Pseudomonas.

Authors:  D B Rajini Rani; A Mahadevan
Journal:  Biometals       Date:  1992       Impact factor: 2.949

8.  Synthetic oligonucleotide probes for detection of mercury-resistance genes in environmental freshwater microbial communities in response to pollutants.

Authors:  I Mirgain; C Hagnere; G A Green; C Harf; H Monteil
Journal:  World J Microbiol Biotechnol       Date:  1992-01       Impact factor: 3.312

9.  Tributyltin-resistant marine bacteria: a summary of recent work.

Authors:  S Suzuki; T Fukagawa
Journal:  J Ind Microbiol       Date:  1995-02

10.  Molecular analysis of mercury-resistant Bacillus isolates from sediment of Minamata Bay, Japan.

Authors:  K Nakamura; S Silver
Journal:  Appl Environ Microbiol       Date:  1994-12       Impact factor: 4.792
View more

北京卡尤迪生物科技股份有限公司 © 2022-2023.