Literature DB >> 1444376

Amplification of DNA from native populations of soil bacteria by using the polymerase chain reaction.

K D Bruce1, W D Hiorns, J L Hobman, A M Osborn, P Strike, D A Ritchie.   

Abstract

Specific DNA sequences from native bacterial populations present in soil, sediment, and sand samples were amplified by using the polymerase chain reaction with primers for either "universal" eubacterial 16S rRNA genes or mercury resistance (mer) genes. With standard amplification conditions, 1.5-kb rDNA fragments from all 12 samples examined and from as little as 5 micrograms of soil were reproducibly amplified. A 1-kb mer fragment from one soil sample was also amplified. The identity of these amplified fragments was confirmed by DNA-DNA hybridization.

Entities:  

Mesh：

Substances：

Year:  1992        PMID： 1444376      PMCID： PMC183114          DOI： 10.1128/aem.58.10.3413-3416.1992

Source DB:  PubMed          Journal:  Appl Environ Microbiol        ISSN： 0099-2240            Impact factor:   4.792


  20 in total

1.  Direct phenotypic and genotypic detection of a recombinant pseudomonad population released into lake water.

Authors:  J A Morgan; C Winstanley; R W Pickup; J G Jones; J R Saunders
Journal:  Appl Environ Microbiol       Date:  1989-10       Impact factor: 4.792

Review 2.  Enumeration and identification of bacteria from environmental samples using nucleic acid probes.

Authors:  T C Hazen; L Jiménez
Journal:  Microbiol Sci       Date:  1988-11

Review 3.  Adaptation of aquatic microbial communities to pollutant stress.

Authors:  T Barkay; H Pritchard
Journal:  Microbiol Sci       Date:  1988-06

4.  High diversity in DNA of soil bacteria.

Authors:  V Torsvik; J Goksøyr; F L Daae
Journal:  Appl Environ Microbiol       Date:  1990-03       Impact factor: 4.792

5.  Isolation and direct complete nucleotide determination of entire genes. Characterization of a gene coding for 16S ribosomal RNA.

Authors:  U Edwards; T Rogall; H Blöcker; M Emde; E C Böttger
Journal:  Nucleic Acids Res       Date:  1989-10-11       Impact factor: 16.971

6.  Recovery of DNA from soils and sediments.

Authors:  R J Steffan; J Goksøyr; A K Bej; R M Atlas
Journal:  Appl Environ Microbiol       Date:  1988-12       Impact factor: 4.792

7.  DNA amplification to enhance detection of genetically engineered bacteria in environmental samples.

Authors:  R J Steffan; R M Atlas
Journal:  Appl Environ Microbiol       Date:  1988-09       Impact factor: 4.792

8.  "A technique for radiolabeling DNA restriction endonuclease fragments to high specific activity". Addendum.

Authors:  A P Feinberg; B Vogelstein
Journal:  Anal Biochem       Date:  1984-02       Impact factor: 3.365

9.  Response of marine bacterioplankton to differential filtration and confinement.

Authors:  R L Ferguson; E N Buckley; A V Palumbo
Journal:  Appl Environ Microbiol       Date:  1984-01       Impact factor: 4.792

10.  Restriction pattern and polypeptide homology among plasmid-borne mercury resistance determinants.

Authors:  M G Jobling; S E Peters; D A Ritchie
Journal:  Plasmid       Date:  1988-09       Impact factor: 3.466
View more
  50 in total

1.  Estimation of bacterial cell numbers in humic acid-rich salt marsh sediments with probes directed to 16S ribosomal DNA

Authors: 
Journal:  Appl Environ Microbiol       Date:  1999-04       Impact factor: 4.792

2.  Presence of bacterial phage-like DNA sequences in commercial Taq DNA polymerase reagents.

Authors:  Tamara Newsome; Bing-Jie Li; Nianxiang Zou; Shyh-Ching Lo
Journal:  J Clin Microbiol       Date:  2004-05       Impact factor: 5.948

3.  Molecular characterization and geological microenvironment of a microbial community inhabiting weathered receding shale cliffs.

Authors:  Charles S Cockell; David Pybus; Karen Olsson-Francis; Laura Kelly; David Petley; Nick Rosser; Kieren Howard; Fred Mosselmans
Journal:  Microb Ecol       Date:  2010-08-04       Impact factor: 4.552

4.  Influence of forest trees on the distribution of mineral weathering-associated bacterial communities of the Scleroderma citrinum mycorrhizosphere.

Authors:  Christophe Calvaruso; Marie-Pierre Turpault; Elisabeth Leclerc; Jacques Ranger; Jean Garbaye; Stéphane Uroz; Pascale Frey-Klett
Journal:  Appl Environ Microbiol       Date:  2010-05-28       Impact factor: 4.792

5.  Comparison of barley succession and take-all disease as environmental factors shaping the rhizobacterial community during take-all decline.

Authors:  Karin Schreiner; Alexandra Hagn; Martina Kyselková; Yvan Moënne-Loccoz; Gerhard Welzl; Jean Charles Munch; Michael Schloter
Journal:  Appl Environ Microbiol       Date:  2010-06-04       Impact factor: 4.792

6.  Potential of a 16S rRNA-based taxonomic microarray for analyzing the rhizosphere effects of maize on Agrobacterium spp. and bacterial communities.

Authors:  Hervé Sanguin; Benoît Remenant; Arnaud Dechesne; Jean Thioulouse; Timothy M Vogel; Xavier Nesme; Yvan Moënne-Loccoz; Geneviève L Grundmann
Journal:  Appl Environ Microbiol       Date:  2006-06       Impact factor: 4.792

7.  Autoradiographic method for isolation of diverse microbial species with unique catabolic traits.

Authors:  J Dunbar; D Wong; M J Yarus; L J Forney
Journal:  Appl Environ Microbiol       Date:  1996-11       Impact factor: 4.792

8.  Bacterial diversity of weathered terrestrial Icelandic volcanic glasses.

Authors:  Laura C Kelly; Charles S Cockell; Yvette M Piceno; Gary L Andersen; Thorsteinn Thorsteinsson; Viggo Marteinsson
Journal:  Microb Ecol       Date:  2010-05-15       Impact factor: 4.552

9.  Analysis of mer Gene Subclasses within Bacterial Communities in Soils and Sediments Resolved by Fluorescent-PCR-Restriction Fragment Length Polymorphism Profiling.

Authors:  K D Bruce
Journal:  Appl Environ Microbiol       Date:  1997-12       Impact factor: 4.792

10.  Genetic Diversity through the Looking Glass: Effect of Enrichment Bias.

Authors:  J Dunbar; S White; L Forney
Journal:  Appl Environ Microbiol       Date:  1997-04       Impact factor: 4.792
View more

北京卡尤迪生物科技股份有限公司 © 2022-2023.