| Literature DB >> 27640192 |
Bingming Ou1,2, Ying Yang1,2, Wai Liang Tham1,2,3, Lin Chen1,2,4, Jitao Guo5, Guoqiang Zhu6,7.
Abstract
Escherichia coli strain Nissle 1917 (EcN) has been used as a probiotic. Genetic engineering has enhanced the utility of EcN in several vaccine and pharmaceutical preparations. We discuss in this mini review the genetics and physical properties of EcN. We also discuss the numerous genetic engineering strategies employed for EcN-based vaccine development, including recombinant plasmid transfer, genetic engineering of cryptic plasmids or the EcN chromosome, EcN bacterial ghosts and its outer membrane vesicles. We also provide a current update on the progress and the challenges regarding the use of EcN in vaccine development.Entities:
Keywords: Chromosome; Clinical application; Cryptic plasmids; Genetic modification; Nissle 1917
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Year: 2016 PMID： 27640192 DOI： 10.1007/s00253-016-7829-5
Source DB: PubMed Journal: Appl Microbiol Biotechnol ISSN： 0175-7598 Impact factor: 4.813