Amrita Pawar1, Sandeep Garg2, Sonia Mehta3, Rajat Dang4. 1. Senior Lecturer, Department of Prosthodontics, ITS College of Dental Sciences & Research , Muradnagar, Ghaziabad, UP, India . 2. Professor and Head, Department of Prosthodontics, MM College of Dental Sciences & Research , Mullana, Ambala, Haryana, India . 3. Associate Professor, Department of Microbiology, MM College of Medical Sciences & Research , Mullana, Ambala, Haryana, India . 4. Professor, Department of Prosthodontics, MM College of Dental Sciences & Research , Mullana, Ambala, Haryana, India .
Abstract
INTRODUCTION: The air-water syringes, ultrasonic scalers, high speed air turbine handpieces are connected to dental units by a network of small-bore plastic tubes through which water and air travel to activate or cool the instruments and it had been shown that this system is extensively contaminated with microbial biofilms and pose a potential risk of infection for patients as well as dental professionals. AIM: To evaluate and compare the efficacy of various disinfectants in reducing the microbial colony count in water derived from Dental Unit Waterlines. MATERIALS AND METHODS: Five random dental units were selected and samples were collected before and after intervention with 5 disinfectants (0.02% H2O2 continuously, 0.02% H2O2 continuously with shock treatment with 0.25% H2O2 weekly, 0.12% Chlorohexidine and 12% Ethanol overnight, 1:50 Original Listerine overnight, 2% Sodium Perborate and 2% EDTA 5 minutes in morning) using different disinfection methods for 4 weeks. Samples were cultured on Reasoner's 2A (R2A) agar for microbial counting. RESULTS: Results were recorded as Colony forming units/ml (cfu/ml) and were evaluated statistically. Results showed that all the dental unit waterlines were heavily contaminated with microbes before any intervention. After 1 day of disinfection regime the counts reduced significantly and showed progressive reduction in consecutive weeks. Goals set by ADA & CDC were ultimately achieved at the end of 4 weeks. CONCLUSION: All the disinfectants were equally effective in reducing the microbial colony count of DUWLs, irrespective of their concentration and method of disinfection.
INTRODUCTION: The air-water syringes, ultrasonic scalers, high speed air turbine handpieces are connected to dental units by a network of small-bore plastic tubes through which water and air travel to activate or cool the instruments and it had been shown that this system is extensively contaminated with microbial biofilms and pose a potential risk of infection for patients as well as dental professionals. AIM: To evaluate and compare the efficacy of various disinfectants in reducing the microbial colony count in water derived from Dental Unit Waterlines. MATERIALS AND METHODS: Five random dental units were selected and samples were collected before and after intervention with 5 disinfectants (0.02% H2O2 continuously, 0.02% H2O2 continuously with shock treatment with 0.25% H2O2 weekly, 0.12% Chlorohexidine and 12% Ethanol overnight, 1:50 Original Listerine overnight, 2% Sodium Perborate and 2% EDTA 5 minutes in morning) using different disinfection methods for 4 weeks. Samples were cultured on Reasoner's 2A (R2A) agar for microbial counting. RESULTS: Results were recorded as Colony forming units/ml (cfu/ml) and were evaluated statistically. Results showed that all the dental unit waterlines were heavily contaminated with microbes before any intervention. After 1 day of disinfection regime the counts reduced significantly and showed progressive reduction in consecutive weeks. Goals set by ADA & CDC were ultimately achieved at the end of 4 weeks. CONCLUSION: All the disinfectants were equally effective in reducing the microbial colony count of DUWLs, irrespective of their concentration and method of disinfection.
Entities:
Keywords:
Dental unit waterlines; Disinfection; Hydrogen peroxide; Infection control
Authors: Giuseppe Fleres; Natacha Couto; Mariette Lokate; Luc W M van der Sluis; Christophe Ginevra; Sophie Jarraud; Ruud H Deurenberg; John W Rossen; Silvia García-Cobos; Alex W Friedrich Journal: Microorganisms Date: 2018-07-18
Authors: Alexandre Baudet; Julie Lizon; Jean-Marc Martrette; Frédéric Camelot; Arnaud Florentin; Céline Clément Journal: Int J Environ Res Public Health Date: 2020-04-12 Impact factor: 3.390