Literature DB >> 2762324

Chromosome mapping of the human gene encoding the 68-kDa nuclear antigen (p68) by using the polymerase chain reaction.

R Iggo1, A Gough, W Xu, D P Lane, N K Spurr.   

Abstract

Chromosome mapping by Southern analysis of DNA from somatic cell hybrids is often unsuccessful when only cDNA probes are available. p68 is a putative RNA helicase that is antigenically related to the simian virus 40 large tumor antigen. By using the polymerase chain reaction and oligode-oxynucleotide primers based on the cDNA sequence, we have identified introns in the p68 gene. Comparison of human and mouse DNA fragments amplified with these primers revealed length differences that allowed us to identify the human gene. Application of this technique to DNA from human-mouse somatic cell hybrids and cell lines derived from them by chromosome-mediated gene transfer allowed us to map p68 to the distal part of the long arm of chromosome 17.

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Year:  1989        PMID： 2762324      PMCID： PMC297807          DOI： 10.1073/pnas.86.16.6211

Source DB:  PubMed          Journal:  Proc Natl Acad Sci U S A        ISSN： 0027-8424            Impact factor:   11.205


  21 in total

1.  Chromosome assignment of some human enzyme loci: mitochondrial malate dehydrogenase to 7, mannosephosphate isomerase and pyruvate kinase to 15 and probably, esterase D to 13.

Authors:  V van Heyningen; M Bobrow; W F Bodmer; S E Gardiner; S Povey; D A Hopkinson
Journal:  Ann Hum Genet       Date:  1975-01       Impact factor: 1.670

Review 2.  A survey on intron and exon lengths.

Authors:  J D Hawkins
Journal:  Nucleic Acids Res       Date:  1988-11-11       Impact factor: 16.971

3.  An eIF-4A-like protein is a suppressor of an Escherichia coli mutant defective in 50S ribosomal subunit assembly.

Authors:  K Nishi; F Morel-Deville; J W Hershey; T Leighton; J Schnier
Journal:  Nature       Date:  1988-12-01       Impact factor: 49.962

4.  Assignment of the human acid alpha-glucosidase gene (alphaGLU) to chromosome 17 using somatic cell hybrids.

Authors:  E Solomon; D Swallow; S Burgess; L Evans
Journal:  Ann Hum Genet       Date:  1979-01       Impact factor: 1.670

5.  A monoclonal antibody recognizing a cell surface antigen coded for by a gene on human chromosome 17.

Authors:  Y Bai; D Sheer; L Hiorns; R W Knowles; A Tunnacliffe
Journal:  Ann Hum Genet       Date:  1982-10       Impact factor: 1.670

6.  Immunochemical analysis of the N-acetyl hexosaminidases in human-mouse hybrids made using a double selective system.

Authors:  D M Swallow; E Solomon; L Pajunen
Journal:  Cytogenet Cell Genet       Date:  1977

7.  Assignment of the human locus determining phosphoglycolate phosphatase (PGP) to chromosome 16.

Authors:  S Povey; S J Jeremiah; R F Barker; D A Hopkinson; E B Robson; P J Cook; E Solomon; M Bobrow; B Carritt; K E Buckton
Journal:  Ann Hum Genet       Date:  1980-01       Impact factor: 1.670

8.  SV40 large T shares an antigenic determinant with a cellular protein of molecular weight 68,000.

Authors:  D P Lane; W K Hoeffler
Journal:  Nature       Date:  1980-11-13       Impact factor: 49.962

9.  Confirmation and further regional assignment of aminoacylase 1 (acy-1) on human chromosome 3 using a simplified detection method.

Authors:  R Voss; I Lerer; S Povey; E Solomon; M Bobrow
Journal:  Ann Hum Genet       Date:  1980-07       Impact factor: 1.670

10.  Human gene mapping using an X/autosome translocation.

Authors:  E Solomon; M Bobrow; P N Goodfellow; W F Bodmer; D M Swallow; S Povey; B Noël
Journal:  Somatic Cell Genet       Date:  1976-03
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  9 in total

1.  Structure and expression of the human p68 RNA helicase gene.

Authors:  O G Rössler; P Hloch; N Schütz; T Weitzenegger; H Stahl
Journal:  Nucleic Acids Res       Date:  2000-02-15       Impact factor: 16.971

2.  Use of 3' untranslated sequences of human cDNAs for rapid chromosome assignment and conversion to STSs: implications for an expression map of the genome.

Authors:  A S Wilcox; A S Khan; J A Hopkins; J M Sikela
Journal:  Nucleic Acids Res       Date:  1991-04-25       Impact factor: 16.971

3.  Cloning, expression and localization of an RNA helicase gene from a human lymphoid cell line with chromosomal breakpoint 11q23.3.

Authors:  D Lu; J J Yunis
Journal:  Nucleic Acids Res       Date:  1992-04-25       Impact factor: 16.971

4.  A novel RNA helicase gene tightly linked to the Triplo-lethal locus of Drosophila.

Authors:  D R Dorer; A C Christensen; D H Johnson
Journal:  Nucleic Acids Res       Date:  1990-09-25       Impact factor: 16.971

5.  A somatic cell hybrid map of the long arm of human chromosome 17, containing the familial breast cancer locus (BRCA1).

Authors:  D M Black; H Nicolai; J Borrow; E Solomon
Journal:  Am J Hum Genet       Date:  1993-04       Impact factor: 11.025

6.  Assignment of eight loci to bovine syntenic groups by use of PCR: extension of a comparative gene map.

Authors:  A B Dietz; H L Neibergs; J E Womack
Journal:  Mamm Genome       Date:  1992       Impact factor: 2.957

7.  Molecular cloning and characterization of a novel human ribonuclease (RNase k6): increasing diversity in the enlarging ribonuclease gene family.

Authors:  H F Rosenberg; K D Dyer
Journal:  Nucleic Acids Res       Date:  1996-09-15       Impact factor: 16.971

8.  Human ribonuclease 4 (RNase 4): coding sequence, chromosomal localization and identification of two distinct transcripts in human somatic tissues.

Authors:  H F Rosenberg; K D Dyer
Journal:  Nucleic Acids Res       Date:  1995-11-11       Impact factor: 16.971

Review 9.  DEAD-Box RNA Helicases DDX3X and DDX5 as Oncogenes or Oncosuppressors: A Network Perspective.

Authors:  Massimiliano Secchi; Camilla Lodola; Anna Garbelli; Silvia Bione; Giovanni Maga
Journal:  Cancers (Basel)       Date:  2022-08-06       Impact factor: 6.575
  9 in total

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