Xiao-Yong Zhang1,2, Feng Wang1,2, Tao Jin3, Junzhong Xu1,2,4,5, Jingping Xie1,2, Daniel F Gochberg1,2,4, John C Gore1,2,3,4,6, Zhongliang Zu1,2. 1. Vanderbilt University Institute of Imaging Science, Vanderbilt University, Nashville, Tennessee, USA. 2. Department of Radiology and Radiological Sciences, Vanderbilt University, Nashville, Tennessee, USA. 3. Department of Radiology, University of Pittsburgh, Vanderbilt University, Nashville, Tennessee, USA. 4. Deparment of Physics and Astronomy, Vanderbilt University, Nashville, Tennessee, USA. 5. Department of Biomedical Engineering, Vanderbilt University, Nashville, Tennessee, USA. 6. Department of Molecular Physiology and Biophysics, Vanderbilt University, Nashville, Tennessee, USA.
Abstract
PURPOSE: To detect, map, and quantify a novel nuclear Overhauser enhancement (NOE)-mediated magnetization transfer (MT) with water at approximately -1.6 ppm [NOE(-1.6)] in rat brain using MRI. METHODS: Continuous wave MT sequences with a variety of radiofrequency irradiation powers were optimized to achieve the maximum contrast of this NOE(-1.6) effect at 9.4 T. The distribution of effect magnitudes, resonance frequency offsets, and line widths in healthy rat brains and the differences of the effect between tumors and contralateral normal brains were imaged and quantified using a multi-Lorentzian fitting method. MR measurements on reconstituted model phospholipids as well as two cell lines (HEK293 and 9L) were also performed to investigate the possible molecular origin of this NOE. RESULTS: Our results suggest that the NOE(-1.6) effect can be detected reliably in rat brain. Pixel-wise fittings demonstrated the regional variations of the effect. Measurements in a rodent tumor model showed that the amplitude of NOE(-1.6) in brain tumor was significantly diminished compared with that in normal brain tissue. Measurements of reconstituted phospholipids suggest that this effect may originate from choline phospholipids. CONCLUSION: NOE(-1.6) could be used as a new biomarker for the detection of brain tumor. Magn Reson Med 78:588-597, 2017.
PURPOSE: To detect, map, and quantify a novel nuclear Overhauser enhancement (NOE)-mediated magnetization transfer (MT) with water at approximately -1.6 ppm [NOE(-1.6)] in rat brain using MRI. METHODS: Continuous wave MT sequences with a variety of radiofrequency irradiation powers were optimized to achieve the maximum contrast of this NOE(-1.6) effect at 9.4 T. The distribution of effect magnitudes, resonance frequency offsets, and line widths in healthy rat brains and the differences of the effect between tumors and contralateral normal brains were imaged and quantified using a multi-Lorentzian fitting method. MR measurements on reconstituted model phospholipids as well as two cell lines (HEK293 and 9L) were also performed to investigate the possible molecular origin of this NOE. RESULTS: Our results suggest that the NOE(-1.6) effect can be detected reliably in rat brain. Pixel-wise fittings demonstrated the regional variations of the effect. Measurements in a rodent tumor model showed that the amplitude of NOE(-1.6) in brain tumor was significantly diminished compared with that in normal brain tissue. Measurements of reconstituted phospholipids suggest that this effect may originate from choline phospholipids. CONCLUSION:NOE(-1.6) could be used as a new biomarker for the detection of brain tumor. Magn Reson Med 78:588-597, 2017.
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