Literature DB >> 27595864

Detection of Isoforms Differing by a Single Charge Unit in Individual Cells.

Augusto M Tentori1,2, Kevin A Yamauchi1, Amy E Herr3,4.   

Abstract

To measure protein isoforms in individual mammalian cells, we report single-cell resolution isoelectric focusing (scIEF) and high-selectivity immunoprobing. Microfluidic design and photoactivatable materials establish the tunable pH gradients required by IEF and precisely control the transport and handling of each 17-pL cell lysate during analysis. The scIEF assay resolves protein isoforms with resolution down to a single-charge unit, including both endogenous cytoplasmic and nuclear proteins from individual mammalian cells.
© 2016 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.

Entities:  

Keywords:  immunoassays; isoelectric focusing; lab-on-a-chip; proteomics; single-cell analysis

Mesh：

Substances：

Year:  2016        PMID： 27595864      PMCID： PMC5201312          DOI： 10.1002/anie.201606039

Source DB:  PubMed          Journal:  Angew Chem Int Ed Engl        ISSN： 1433-7851            Impact factor:   15.336


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