| Literature DB >> 27595345 |
Ming Ni1, Chen Chen2,3, Jun Qian4, Hai-Xia Xiao5,6, Wei-Feng Shi7, Yang Luo8, Hai-Yin Wang2, Zhen Li1, Jun Wu5, Pei-Song Xu9, Su-Hong Chen1, Gary Wong5, Yuhai Bi5,10, Zhi-Ping Xia4, Wei Li11, Hui-Jun Lu4, Juncai Ma11, Yi-Gang Tong12, Hui Zeng2,3, Sheng-Qi Wang1, George F Gao5,10,13, Xiao-Chen Bo1, Di Liu5,11.
Abstract
Since 2013, West Africa has encountered the largest Ebola virus (EBOV) disease outbreak on record, and Sierra Leone is the worst-affected country, with nearly half of the infections. By means of next-generation sequencing and phylogeographic analysis, the epidemiology and transmission of EBOV have been well elucidated. However, the intra-host dynamics that mainly reflect viral-host interactions still need to be studied. Here, we show a total of 710 intra-host single nucleotide variations (iSNVs) from deep-sequenced samples from EBOV-infected patients, through a well-tailored bioinformatics pipeline. We present a comprehensive distribution of iSNVs during this outbreak and along the EBOV genome. Analyses of iSNV and its allele frequency reveal that VP40 is the most conserved gene during this outbreak, and thus it would be an ideal therapeutic target. In the co-occurring iSNV network, varied iSNV sites present different selection features. Intriguingly, the T-to-C substitutions at the 3'-UTR of the nucleoprotein (NP; positions 3008 and 3011), observed in many patients, result in the upregulation of the transcription of NP through an Ebola mini-genome reporting system. Additionally, no iSNV enrichment within B-cell epitopes of GP has been observed.Entities:
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Year: 2016 PMID： 27595345 DOI： 10.1038/nmicrobiol.2016.151
Source DB: PubMed Journal: Nat Microbiol ISSN： 2058-5276 Impact factor: 17.745