| Literature DB >> 26083749 |
Miles W Carroll1, David A Matthews2, Julian A Hiscox3, Michael J Elmore4, Georgios Pollakis3, Andrew Rambaut5, Roger Hewson6, Isabel García-Dorival3, Joseph Akoi Bore7, Raymond Koundouno7, Saïd Abdellati8, Babak Afrough9, John Aiyepada10, Patience Akhilomen10, Danny Asogun10, Barry Atkinson9, Marlis Badusche11, Amadou Bah12, Simon Bate9, Jan Baumann13, Dirk Becker14, Beate Becker-Ziaja11, Anne Bocquin15, Benny Borremans16, Andrew Bosworth17, Jan Peter Boettcher18, Angela Cannas19, Fabrizio Carletti19, Concetta Castilletti19, Simon Clark9, Francesca Colavita19, Sandra Diederich20, Adomeh Donatus10, Sophie Duraffour21, Deborah Ehichioya22, Heinz Ellerbrok18, Maria Dolores Fernandez-Garcia23, Alexandra Fizet24, Erna Fleischmann25, Sophie Gryseels16, Antje Hermelink18, Julia Hinzmann18, Ute Hopf-Guevara18, Yemisi Ighodalo10, Lisa Jameson9, Anne Kelterbaum14, Zoltan Kis26, Stefan Kloth18, Claudia Kohl18, Miša Korva27, Annette Kraus28, Eeva Kuisma9, Andreas Kurth18, Britta Liedigk11, Christopher H Logue9, Anja Lüdtke29, Piet Maes30, James McCowen9, Stéphane Mély15, Marc Mertens20, Silvia Meschi19, Benjamin Meyer31, Janine Michel18, Peter Molkenthin25, César Muñoz-Fontela29, Doreen Muth31, Edmund N C Newman9, Didier Ngabo9, Lisa Oestereich11, Jennifer Okosun10, Thomas Olokor10, Racheal Omiunu10, Emmanuel Omomoh10, Elisa Pallasch11, Bernadett Pályi26, Jasmine Portmann32, Thomas Pottage9, Catherine Pratt9, Simone Priesnitz33, Serena Quartu19, Julie Rappe34, Johanna Repits35, Martin Richter18, Martin Rudolf11, Andreas Sachse18, Kristina Maria Schmidt18, Gordian Schudt14, Thomas Strecker14, Ruth Thom9, Stephen Thomas9, Ekaete Tobin10, Howard Tolley9, Jochen Trautner36, Tine Vermoesen8, Inês Vitoriano9, Matthias Wagner25, Svenja Wolff14, Constanze Yue18, Maria Rosaria Capobianchi19, Birte Kretschmer37, Yper Hall4, John G Kenny38, Natasha Y Rickett3, Gytis Dudas39, Cordelia E M Coltart40, Romy Kerber11, Damien Steer41, Callum Wright42, Francis Senyah4, Sakoba Keita43, Patrick Drury44, Boubacar Diallo45, Hilde de Clerck46, Michel Van Herp46, Armand Sprecher46, Alexis Traore47, Mandiou Diakite48, Mandy Kader Konde49, Lamine Koivogui50, N'Faly Magassouba51, Tatjana Avšič-Županc27, Andreas Nitsche18, Marc Strasser32, Giuseppe Ippolito19, Stephan Becker14, Kilian Stoecker25, Martin Gabriel11, Hervé Raoul52, Antonino Di Caro19, Roman Wölfel25, Pierre Formenty44, Stephan Günther11.
Abstract
West Africa is currently witnessing the most extensive Ebola virus (EBOV) outbreak so far recorded. Until now, there have been 27,013 reported cases and 11,134 deaths. The origin of the virus is thought to have been a zoonotic transmission from a bat to a two-year-old boy in December 2013 (ref. 2). From this index case the virus was spread by human-to-human contact throughout Guinea, Sierra Leone and Liberia. However, the origin of the particular virus in each country and time of transmission is not known and currently relies on epidemiological analysis, which may be unreliable owing to the difficulties of obtaining patient information. Here we trace the genetic evolution of EBOV in the current outbreak that has resulted in multiple lineages. Deep sequencing of 179 patient samples processed by the European Mobile Laboratory, the first diagnostics unit to be deployed to the epicentre of the outbreak in Guinea, reveals an epidemiological and evolutionary history of the epidemic from March 2014 to January 2015. Analysis of EBOV genome evolution has also benefited from a similar sequencing effort of patient samples from Sierra Leone. Our results confirm that the EBOV from Guinea moved into Sierra Leone, most likely in April or early May. The viruses of the Guinea/Sierra Leone lineage mixed around June/July 2014. Viral sequences covering August, September and October 2014 indicate that this lineage evolved independently within Guinea. These data can be used in conjunction with epidemiological information to test retrospectively the effectiveness of control measures, and provides an unprecedented window into the evolution of an ongoing viral haemorrhagic fever outbreak.Entities:
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Year: 2015 PMID： 26083749 DOI： 10.1038/nature14594
Source DB: PubMed Journal: Nature ISSN： 0028-0836 Impact factor: 49.962