Literature DB >> 27570799

Isolation, Culture, and Maintenance of Mouse Intestinal Stem Cells.

Kevin P O'Rourke1, Sarah Ackerman2, Lukas E Dow3, Scott W Lowe4.   

Abstract

In this protocol we describe our modifications to a method to isolate, culture and maintain mouse intestinal stem cells as crypt-villus forming organoids. These cells, isolated either from the small or large intestine, maintain self-renewal and multilineage differentiation potential over time. This provides investigators a tool to culture wild type or transformed intestinal epithelium, and a robust assay for stem cell tissue homeostasis in vitro.

Entities:  

Year:  2016        PMID： 27570799      PMCID： PMC4996657          DOI： 10.21769/bioprotoc.1733

Source DB:  PubMed          Journal:  Bio Protoc        ISSN： 2331-8325


  4 in total

1.  2000 Report of the AVMA Panel on Euthanasia.

Authors: 
Journal:  J Am Vet Med Assoc       Date:  2001-03-01       Impact factor: 1.936

2.  Single Lgr5 stem cells build crypt-villus structures in vitro without a mesenchymal niche.

Authors:  Toshiro Sato; Robert G Vries; Hugo J Snippert; Marc van de Wetering; Nick Barker; Daniel E Stange; Johan H van Es; Arie Abo; Pekka Kujala; Peter J Peters; Hans Clevers
Journal:  Nature       Date:  2009-03-29       Impact factor: 49.962

3.  Long-term expansion of epithelial organoids from human colon, adenoma, adenocarcinoma, and Barrett's epithelium.

Authors:  Toshiro Sato; Daniel E Stange; Marc Ferrante; Robert G J Vries; Johan H Van Es; Stieneke Van den Brink; Winan J Van Houdt; Apollo Pronk; Joost Van Gorp; Peter D Siersema; Hans Clevers
Journal:  Gastroenterology       Date:  2011-09-02       Impact factor: 22.682

4.  Identification of stem cells in small intestine and colon by marker gene Lgr5.

Authors:  Nick Barker; Johan H van Es; Jeroen Kuipers; Pekka Kujala; Maaike van den Born; Miranda Cozijnsen; Andrea Haegebarth; Jeroen Korving; Harry Begthel; Peter J Peters; Hans Clevers
Journal:  Nature       Date:  2007-10-14       Impact factor: 49.962
  4 in total
  23 in total

1.  In Vitro Generation of Mouse Colon Crypts.

Authors:  Yuli Wang; Dulan B Gunasekara; Peter J Attayek; Mark I Reed; Matthew DiSalvo; Daniel L Nguyen; Johanna S Dutton; Michael S Lebhar; Scott J Bultman; Christopher E Sims; Scott T Magness; Nancy L Allbritton
Journal:  ACS Biomater Sci Eng       Date:  2017-08-29

2.  The Misshapen subfamily of Ste20 kinases regulate proliferation in the aging mammalian intestinal epithelium.

Authors:  Qi Li; Niraj K Nirala; Hsi-Ju Chen; Yingchao Nie; Wei Wang; Biliang Zhang; Michael P Czech; Qi Wang; Lan Xu; Junhao Mao; Y Tony Ip
Journal:  J Cell Physiol       Date:  2019-05-01       Impact factor: 6.384

3.  Metformin mitigates gastrointestinal radiotoxicity and radiosensitises P53 mutation colorectal tumours via optimising autophagy.

Authors:  Long Chen; Fengying Liao; Zhongyong Jiang; Chi Zhang; Ziwen Wang; Peng Luo; Qingzhi Jiang; Jie Wu; Qing Wang; Min Luo; Xueru Li; Yu Leng; Le Ma; Gufang Shen; Zelin Chen; Yu Wang; Xu Tan; Yibo Gan; Dengqun Liu; Yunsheng Liu; Chunmeng Shi
Journal:  Br J Pharmacol       Date:  2020-07-08       Impact factor: 8.739

4.  Distinct Colorectal Cancer-Associated APC Mutations Dictate Response to Tankyrase Inhibition.

Authors:  Emma M Schatoff; Sukanya Goswami; Maria Paz Zafra; Miguel Foronda; Michael Shusterman; Benjamin I Leach; Alyna Katti; Bianca J Diaz; Lukas E Dow
Journal:  Cancer Discov       Date:  2019-07-23       Impact factor: 39.397

5.  Preparation and Cultivation of Colonic and Small Intestinal Murine Organoids Including Analysis of Gene Expression and Organoid Viability.

Authors:  Luisa Klemke; Joshua P Blume; Tiago De Oliveira; Ramona Schulz-Heddergott
Journal:  Bio Protoc       Date:  2022-01-20

Review 6.  Base editing the mammalian genome.

Authors:  Emma M Schatoff; Maria Paz Zafra; Lukas E Dow
Journal:  Methods       Date:  2019-03-02       Impact factor: 3.608

7.  A Unique Nonsaccharide Mimetic of Heparin Hexasaccharide Inhibits Colon Cancer Stem Cells via p38 MAP Kinase Activation.

Authors:  Rio S Boothello; Nirmita J Patel; Chetna Sharon; Elsamani I Abdelfadiel; Shravan Morla; Donald F Brophy; H Robert Lippman; Umesh R Desai; Bhaumik B Patel
Journal:  Mol Cancer Ther       Date:  2018-10-18       Impact factor: 6.261

Review 8.  Patient-derived organoid models help define personalized management of gastrointestinal cancer.

Authors:  M R Aberle; R A Burkhart; H Tiriac; S W M Olde Damink; C H C Dejong; D A Tuveson; R M van Dam
Journal:  Br J Surg       Date:  2018-01       Impact factor: 6.939

9.  p300 Serine 89: A Critical Signaling Integrator and Its Effects on Intestinal Homeostasis and Repair.

Authors:  Keane K Y Lai; Xiaohui Hu; Keisuke Chosa; Cu Nguyen; David P Lin; Keith K Lai; Nobuo Kato; Yusuke Higuchi; Sarah K Highlander; Elizabeth Melendez; Yoshihiro Eriguchi; Patrick T Fueger; Andre J Ouellette; Nyam-Osor Chimge; Masaya Ono; Michael Kahn
Journal:  Cancers (Basel)       Date:  2021-03-14       Impact factor: 6.639

10.  Immunofluorescent Staining of Mouse Intestinal Stem Cells.

Authors:  Kevin P O'Rourke; Lukas E Dow; Scott W Lowe
Journal:  Bio Protoc       Date:  2016-02-20
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