Hideo Shigeishi1, Masaru Sugiyama2, Kouji Ohta1, Mohammad Zeshaan Rahman1, Masaaki Takechi1. 1. - Hiroshima University, Institute of Biomedical and Health Sciences, Department of Oral and Maxillofacial Surgery, Hiroshima, Japan. 2. - Hiroshima University, Institute of Biomedical and Health Sciences, Department of Public Oral Health, Hiroshima, Japan.
Abstract
OBJECTIVE: The objective of this study was to clarify differences regarding HPV16 infection and gene amplification between the oral cavity and oropharynx in healthy individuals. MATERIAL AND METHODS: The subjects were 94 healthy asymptomatic individuals (41 males, 53 females; mean age 58.6 years, range 16-97 years) who visited the Department of Oral and Maxillofacial Reconstructive Surgery of the Hiroshima University Hospital from 2014 to 2015. Oral epithelial cells were collected from oral rinse and pharynx gargle samples and placed in saline. The human endogenous retrovirus gene ERV3-1 was used as a reference to estimate the number of human cells in each sample. DNA samples were extracted from approximately 10,000 human cells and tested for HPV16 DNA by PCR using a type-specific primer. Similarly, we analyzed the HPV16 viral copy number in HPV16-positive cases using real-time PCR to examine genomic amplification. RESULTS: The percentage of HPV16-positive cases was higher in the gargle (28.7%) as compared to the rinse (16.0%) samples. In the oral rinse samples, males (26.8%) showed a significantly higher rate of HPV16 than females (7.5%) (P=0.021). Importantly, in older subjects (aged 60-89 years), gargle samples showed a significantly higher rate of HPV16 (33.3%) than oral rinse samples (13.7%) (P=0.034). The average number of viral copies was approximately 8 times higher in the gargle than in the oral rinse samples (0.16±0.27 vs. 1.35±1.26 copy numbers per cell), a significant difference (P<0.001). CONCLUSION: Our findings suggest that the oropharynx is more susceptible to HPV16 infection as compared to the oral cavity, while HPV16 gene amplification is also more commonly found in the oropharynx.
OBJECTIVE: The objective of this study was to clarify differences regarding HPV16infection and gene amplification between the oral cavity and oropharynx in healthy individuals. MATERIAL AND METHODS: The subjects were 94 healthy asymptomatic individuals (41 males, 53 females; mean age 58.6 years, range 16-97 years) who visited the Department of Oral and Maxillofacial Reconstructive Surgery of the Hiroshima University Hospital from 2014 to 2015. Oral epithelial cells were collected from oral rinse and pharynx gargle samples and placed in saline. The human endogenous retrovirus gene ERV3-1 was used as a reference to estimate the number of human cells in each sample. DNA samples were extracted from approximately 10,000 human cells and tested for HPV16 DNA by PCR using a type-specific primer. Similarly, we analyzed the HPV16 viral copy number in HPV16-positive cases using real-time PCR to examine genomic amplification. RESULTS: The percentage of HPV16-positive cases was higher in the gargle (28.7%) as compared to the rinse (16.0%) samples. In the oral rinse samples, males (26.8%) showed a significantly higher rate of HPV16 than females (7.5%) (P=0.021). Importantly, in older subjects (aged 60-89 years), gargle samples showed a significantly higher rate of HPV16 (33.3%) than oral rinse samples (13.7%) (P=0.034). The average number of viral copies was approximately 8 times higher in the gargle than in the oral rinse samples (0.16±0.27 vs. 1.35±1.26 copy numbers per cell), a significant difference (P<0.001). CONCLUSION: Our findings suggest that the oropharynx is more susceptible to HPV16infection as compared to the oral cavity, while HPV16 gene amplification is also more commonly found in the oropharynx.
Authors: Kristina R Dahlstrom; Ann N Burchell; Agnihotram V Ramanakumar; Allita Rodrigues; Pierre-Paul Tellier; James Hanley; François Coutlée; Eduardo L Franco Journal: Cancer Epidemiol Biomarkers Prev Date: 2014-11-12 Impact factor: 4.254
Authors: Marina D M Lima; Paulo Henrique Braz-Silva; Sônia M Pereira; Catalina Riera; Ariane C Coelho; Marina Gallottini Journal: Int J Gynaecol Obstet Date: 2014-04-03 Impact factor: 3.561
Authors: Daniel Dalla Torre; Doris Burtscher; Michael Edlinger; Elisabeth Sölder; Andreas Widschwendter; Michael Rasse; Wolfgang Puelacher Journal: Oral Surg Oral Med Oral Pathol Oral Radiol Date: 2014-12-05
Authors: Moritz F Meyer; Christian U Huebbers; Oliver G Siefer; Julia Vent; Iris Engbert; Guy D Eslick; Markus Valter; Jens Peter Klussmann; Simon F Preuss Journal: Oral Oncol Date: 2013-10-26 Impact factor: 5.337
Authors: James J Yun; Lawrence E Heisler; Irene I L Hwang; Olivia Wilkins; Suzanne K Lau; Martin Hyrcza; Bamini Jayabalasingham; Jing Jin; JoAnne McLaurin; Ming-Sound Tsao; Sandy D Der Journal: Nucleic Acids Res Date: 2006-07-13 Impact factor: 16.971