Literature DB >> 2754009

Comparison of HeLa 229 and McCoy cell cultures for detection of Chlamydia trachomatis in clinical specimens.

E A Thewessen1, I Freundt, J H van Rijsoort-Vos, E Stolz, M F Michel, J H Wagenvoort.   

Abstract

Consecutive clinical specimens of Chlamydia trachomatis (1,048) were inoculated in parallel on DEAE-dextran- and cycloheximide-treated HeLa 229 cells and cycloheximide-treated McCoy cells. HeLa 229 cell culture detected 113 positive specimens, and McCoy cell culture detected 103 positive specimens. This difference is not significant. However, HeLa 229 cell culture yielded significantly more inclusions than McCoy cell culture in the 95 specimens positive in both cell types (P = 0.042). For routine diagnostic purposes, a choice for one of the cell types may be determined by local preferences.

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Year:  1989        PMID： 2754009      PMCID： PMC267569          DOI： 10.1128/jcm.27.6.1399-1400.1989

Source DB:  PubMed          Journal:  J Clin Microbiol        ISSN： 0095-1137            Impact factor:   5.948


  8 in total

1.  Comparison of various McCoy cell treatment procedures used for detection of Chlamydia trachomatis.

Authors:  R T Evans; D Taylor-Robinson
Journal:  J Clin Microbiol       Date:  1979-08       Impact factor: 5.948

2.  Enhancement of yield of Chlamydia trachomatis Hela 229 cell culture.

Authors:  J T Wagenvoort; T van Rijsoort-Vos; A Overkleeft-van de Ree; E Stolz
Journal:  Eur J Clin Microbiol Infect Dis       Date:  1988-12       Impact factor: 3.267

3.  Early detection of chlamydial inclusions combining the use of cycloheximide-treated McCoy cells and immunofluorescence staining.

Authors:  B J Thomas; R T Evans; G R Hutchinson; D Taylor-Robinson
Journal:  J Clin Microbiol       Date:  1977-09       Impact factor: 5.948

4.  Comparison of methods for cultivation and isolation of Chlamydia trachomatis.

Authors:  S Benes; W M McCormack
Journal:  J Clin Microbiol       Date:  1982-11       Impact factor: 5.948

5.  Microtest procedure for isolation of Chlamydia trachomatis.

Authors:  B L Yoder; W E Stamm; C M Koester; E R Alexander
Journal:  J Clin Microbiol       Date:  1981-06       Impact factor: 5.948

6.  Efficacy of various cell culture procedures for detection of Chlamydia trachomatis and applicability to diagnosis of pediatric infections.

Authors:  L J La Scolea; J E Keddell
Journal:  J Clin Microbiol       Date:  1981-04       Impact factor: 5.948

7.  Cultivation of Chlamydia trachomatis in cycloheximide-treated mccoy cells.

Authors:  K T Ripa; P A Mårdh
Journal:  J Clin Microbiol       Date:  1977-10       Impact factor: 5.948

8.  Enhancement of Chlamydia trachomatis infectious progeny by cultivation of HeLa 229 cells treated with DEAE-dextran and cycloheximide.

Authors:  S F Sabet; J Simmons; H D Caldwell
Journal:  J Clin Microbiol       Date:  1984-08       Impact factor: 5.948
  8 in total
  10 in total

1.  Comparison of three commercially available amplification assays, AMP CT, LCx, and COBAS AMPLICOR, for detection of Chlamydia trachomatis in first-void urine.

Authors:  W H Goessens; J W Mouton; W I van der Meijden; S Deelen; T H van Rijsoort-Vos; N Lemmens-den Toom; H A Verbrugh; R P Verkooyen
Journal:  J Clin Microbiol       Date:  1997-10       Impact factor: 5.948

2.  Different doses of cefetamet pivoxil (Ro 15-8075) in the treatment of acute uncomplicated gonococcal urethritis in men.

Authors:  T T Tio; I R Sindhunata; J H Wagenvoort; M F Michel; E Stolz
Journal:  Antimicrob Agents Chemother       Date:  1990-04       Impact factor: 5.191

3.  Bacterial flora of the sigmoid neovagina.

Authors:  T A Toolenaar; I Freundt; J H Wagenvoort; F J Huikeshoven; M Vogel; H Jeekel; A C Drogendijk
Journal:  J Clin Microbiol       Date:  1993-12       Impact factor: 5.948

4.  Screening for cervical Chlamydia trachomatis infections in two Dutch populations.

Authors:  E A Thewessen; W I van der Meijden; H J Doppenberg; P G Mulder; J H Wagenvoort; E Stolz; M F Michel
Journal:  Genitourin Med       Date:  1990-10

5.  Detection of Chlamydia trachomatis in male and female urine specimens by using the amplified Chlamydia trachomatis test.

Authors:  J W Mouton; R Verkooyen; W I van der Meijden; T H van Rijsoort-Vos; W H Goessens; J A Kluytmans; S D Deelen; A Luijendijk; H A Verbrugh
Journal:  J Clin Microbiol       Date:  1997-06       Impact factor: 5.948

6.  Evaluation of Clearview and Magic Lite tests, polymerase chain reaction, and cell culture for detection of Chlamydia trachomatis in urogenital specimens.

Authors:  J A Kluytmans; W H Goessens; J W Mouton; J H van Rijsoort-Vos; H G Niesters; W G Quint; L Habbema; E Stolz; J H Wagenvoort
Journal:  J Clin Microbiol       Date:  1993-12       Impact factor: 5.948

7.  Solid-phase C1q-directed bacterial capture followed by PCR for detection of Chlamydia trachomatis in clinical specimens.

Authors:  P Herbrink; H A van den Munckhof; H G Niesters; W H Goessens; E Stolz; W G Quint
Journal:  J Clin Microbiol       Date:  1995-02       Impact factor: 5.948

8.  Performance of a nonisotopic DNA probe for detection of Chlamydia trachomatis in urogenital specimens.

Authors:  J A Kluytmans; H G Niesters; J W Mouton; W G Quint; J A Ijpelaar; J H Van Rijsoort-Vos; L Habbema; E Stolz; M F Michel; J H Wagenvoort
Journal:  J Clin Microbiol       Date:  1991-12       Impact factor: 5.948

9.  Chlamydia trachomatis-specific antibodies in patients with pelvic inflammatory disease: comparison with isolation in tissue culture or detection with polymerase chain reaction.

Authors:  J J Theunissen; W Minderhoud-Bassie; J H Wagenvoort; E Stolz; M F Michel; F J Huikeshoven
Journal:  Genitourin Med       Date:  1994-10

10.  Diagnostic value of the polymerase chain reaction for Chlamydia detection as determined in a follow-up study.

Authors:  H C Claas; J H Wagenvoort; H G Niesters; T T Tio; J H Van Rijsoort-Vos; W G Quint
Journal:  J Clin Microbiol       Date:  1991-01       Impact factor: 5.948
  10 in total

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