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Literature DB >> 27529518

G Protein-Coupled Receptors Incorporated into Rehydrated Diblock Copolymer Vesicles Retain Functionality.

M Gertrude Gutierrez¹, Farzad Jalali-Yazdi¹, Justin Peruzzi², Carson T Riche¹, Richard W Roberts³, Noah Malmstadt⁴.

Abstract

G protein-coupled receptor (GPCR) is incorporated into polymeric vesicles made up of diblock copolymer bilayers. Successfully incorporated GPCRs exhibit correct biased physiological orientation and respond to various ligands. After extended dehydrated storage via lyophilization and subsequent rehydration, diblock copolymer polymersomes retain their shape and incorporated GPCR retains its function.

Entities: Chemical Disease Gene Species

Keywords: GPCR; bilayer vesicles; block copolymers; lyophilization; polymersomes

Mesh：

Substances：

Year: 2016 PMID： 27529518 PMCID： PMC5148614 DOI： 10.1002/smll.201601540

Source DB: PubMed Journal: Small ISSN： 1613-6810 Impact factor: 13.281

24 in total

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Authors: Robert J Lefkowitz
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2. Impact of sucrose level on storage stability of proteins in freeze-dried solids: II. Correlation of aggregation rate with protein structure and molecular mobility.

Authors: Bingquan Wang; Serguei Tchessalov; Marcus T Cicerone; Nicholas W Warne; Michael J Pikal
Journal: J Pharm Sci Date: 2009-09 Impact factor: 3.534

3. In vitro expressed GPCR inserted in polymersome membranes for ligand-binding studies.

Authors: Sylvia May; Mirjam Andreasson-Ochsner; Zhikang Fu; Ying Xiu Low; Darren Tan; Hans-Peter M de Hoog; Sandra Ritz; Madhavan Nallani; Eva-Kathrin Sinner
Journal: Angew Chem Int Ed Engl Date: 2012-11-19 Impact factor: 15.336

4. Cascade reactions in multicompartmentalized polymersomes.

Authors: Ruud J R W Peters; Maïté Marguet; Sébastien Marais; Marco W Fraaije; Jan C M van Hest; Sébastien Lecommandoux
Journal: Angew Chem Int Ed Engl Date: 2013-11-19 Impact factor: 15.336

5. Hydrogen bonding between sugar and protein is responsible for inhibition of dehydration-induced protein unfolding.

Authors: S D Allison; B Chang; T W Randolph; J F Carpenter
Journal: Arch Biochem Biophys Date: 1999-05-15 Impact factor: 4.013

6. Fragmentation into small vesicles of dioleoylphosphatidylcholine bilayers during freezing and thawing.

Authors: R C MacDonald; F D Jones; R Qiu
Journal: Biochim Biophys Acta Date: 1994-05-11

7. Highly permeable polymeric membranes based on the incorporation of the functional water channel protein Aquaporin Z.

Authors: Manish Kumar; Mariusz Grzelakowski; Julie Zilles; Mark Clark; Wolfgang Meier
Journal: Proc Natl Acad Sci U S A Date: 2007-12-11 Impact factor: 11.205

8. New imide 5-HT1A receptor ligands - modification of terminal fragment geometry.

Authors: Andrzej J Bojarski; Maria J Mokrosz; Beata Duszyńska; Aneta Kozioł; Ryszard Bugno
Journal: Molecules Date: 2004-02-28 Impact factor: 4.411

9. Antibody capture assay reveals bell-shaped concentration-response isotherms for h5-HT(1A) receptor-mediated Galpha(i3) activation: conformational selection by high-efficacy agonists, and relationship to trafficking of receptor signaling.

Authors: Adrian Newman-Tancredi; Didier Cussac; Laetitia Marini; Mark J Millan
Journal: Mol Pharmacol Date: 2002-09 Impact factor: 4.436