Literature DB >> 23161746

In vitro expressed GPCR inserted in polymersome membranes for ligand-binding studies.

Sylvia May1, Mirjam Andreasson-Ochsner, Zhikang Fu, Ying Xiu Low, Darren Tan, Hans-Peter M de Hoog, Sandra Ritz, Madhavan Nallani, Eva-Kathrin Sinner.   

Abstract

The dopamine receptor D2 (DRD2), a G-protein coupled receptor is expressed into PBd(22)-PEO(13) and PMOXA(20)-PDMS(54)-PMOXA(20) block copolymer vesicles. The conformational integrity of the receptor is confirmed by antibody- and ligand-binding assays. Replacement of bound dopamine is demonstrated on surface-immobilized polymersomes, thus making this a promising platform for drug screening.
Copyright © 2013 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.

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Year:  2012        PMID： 23161746     DOI： 10.1002/anie.201204645

Source DB:  PubMed          Journal:  Angew Chem Int Ed Engl        ISSN： 1433-7851            Impact factor:   15.336


  10 in total

1.  Proteo-lipobeads for the oriented encapsulation of membrane proteins.

Authors:  Pinar Frank; Bernhard Siebenhofer; Theresa Hanzer; Andreas F Geiss; Florian Schadauer; Ciril Reiner-Rozman; Bill Durham; Leslie M Loew; Bernd Ludwig; Oliver-M H Richter; Christoph Nowak; Renate L C Naumann
Journal:  Soft Matter       Date:  2015-04-21       Impact factor: 3.679

2.  G Protein-Coupled Receptors Incorporated into Rehydrated Diblock Copolymer Vesicles Retain Functionality.

Authors:  M Gertrude Gutierrez; Farzad Jalali-Yazdi; Justin Peruzzi; Carson T Riche; Richard W Roberts; Noah Malmstadt
Journal:  Small       Date:  2016-08-16       Impact factor: 13.281

3.  Conformational antibody binding to a native, cell-free expressed GPCR in block copolymer membranes.

Authors:  Hans-Peter M de Hoog; Esther M Lin JieRong; Sourabh Banerjee; Fabien M Décaillot; Madhavan Nallani
Journal:  PLoS One       Date:  2014-10-20       Impact factor: 3.240

4.  Discovery of indolylpiperazinylpyrimidines with dual-target profiles at adenosine A2A and dopamine D2 receptors for Parkinson's disease treatment.

Authors:  Yi-Ming Shao; Xiaohua Ma; Priyankar Paira; Aaron Tan; Deron Raymond Herr; Kah Leong Lim; Chee Hoe Ng; Gopalakrishnan Venkatesan; Karl-Norbert Klotz; Stephanie Federico; Giampiero Spalluto; Siew Lee Cheong; Yu Zong Chen; Giorgia Pastorin
Journal:  PLoS One       Date:  2018-01-05       Impact factor: 3.240

Review 5.  Cell-Free Approaches in Synthetic Biology Utilizing Microfluidics.

Authors:  Samar Damiati; Rami Mhanna; Rimantas Kodzius; Eva-Kathrin Ehmoser
Journal:  Genes (Basel)       Date:  2018-03-06       Impact factor: 4.096

6.  A novel optical tracer for VMAT2 applied to live cell measurements of vesicle maturation in cultured human β-cells.

Authors:  Stevan Pecic; Nenad Milosavic; Gina Rayat; Antonella Maffei; Paul E Harris
Journal:  Sci Rep       Date:  2019-04-01       Impact factor: 4.379

7.  I n situ synthesis and unidirectional insertion of membrane proteins in liposome-immobilized silica stationary phase for rapid preparation of microaffinity chromatography.

Authors:  Yanqiu Gu; Rong Wang; Panpan Chen; Shengnan Li; Xinyi Chai; Chun Chen; Yue Liu; Yan Cao; Diya Lv; Zhanying Hong; Zhenyu Zhu; Yifeng Chai; Yongfang Yuan; Xiaofei Chen
Journal:  Acta Pharm Sin B       Date:  2022-04-22       Impact factor: 14.903

8.  Human serotonin receptor 5-HT(1A) preferentially segregates to the liquid disordered phase in synthetic lipid bilayers.

Authors:  M Gertrude Gutierrez; Noah Malmstadt
Journal:  J Am Chem Soc       Date:  2014-09-19       Impact factor: 15.419

9.  Bacterial reaction centers purified with styrene maleic acid copolymer retain native membrane functional properties and display enhanced stability.

Authors:  David J K Swainsbury; Stefan Scheidelaar; Rienk van Grondelle; J Antoinette Killian; Michael R Jones
Journal:  Angew Chem Int Ed Engl       Date:  2014-09-11       Impact factor: 15.336

Review 10.  The hallmarks of living systems: towards creating artificial cells.

Authors:  N Amy Yewdall; Alexander F Mason; Jan C M van Hest
Journal:  Interface Focus       Date:  2018-08-17       Impact factor: 3.906
  10 in total

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