Literature DB >> 27518625

Determination of Histone H2AX Phosphorylation in DT40 Cells.

Kana Nishihara1, Sampada A Shahane1, Menghang Xia2.   

Abstract

Visualization of DNA damage response protein recruitment to DNA damage sites enables measurement of the DNA damage. DNA double-strand breaks (DSBs) and blocked replication forks induce the phosphorylation of H2AX at serine 139 (γH2AX), and accumulate γH2AX which can then be detected as foci. The detection of γH2AX foci by immunostaining with antibodies that recognize γH2AX is an indicator of DSBs presence. This chapter describes the measurement of γH2AX immunostaining using a high-content imaging platform in chicken DT40 B-lymphocyte cell lines.

Entities:  

Keywords:  DSB; High-contents imaging; Immunostaining; γH2AX

Mesh：

Substances：

Year:  2016        PMID： 27518625      PMCID： PMC5375169          DOI： 10.1007/978-1-4939-6346-1_8

Source DB:  PubMed          Journal:  Methods Mol Biol        ISSN： 1064-3745


  8 in total

Review 1.  Reverse genetic studies of the DNA damage response in the chicken B lymphocyte line DT40.

Authors:  Mitsuyoshi Yamazoe; Eiichiro Sonoda; Helfrid Hochegger; Shunichi Takeda
Journal:  DNA Repair (Amst)       Date:  2004 Aug-Sep

Review 2.  Mutant cells defective in DNA repair pathways provide a sensitive high-throughput assay for genotoxicity.

Authors:  Terry John Evans; Kimiyo N Yamamoto; Kouji Hirota; Shunichi Takeda
Journal:  DNA Repair (Amst)       Date:  2010-10-27

3.  Increased ratio of targeted to random integration after transfection of chicken B cell lines.

Authors:  J M Buerstedde; S Takeda
Journal:  Cell       Date:  1991-10-04       Impact factor: 41.582

4.  DNA double-stranded breaks induce histone H2AX phosphorylation on serine 139.

Authors:  E P Rogakou; D R Pilch; A H Orr; V S Ivanova; W M Bonner
Journal:  J Biol Chem       Date:  1998-03-06       Impact factor: 5.157

Review 5.  γ-H2AX as a biomarker for DNA double-strand breaks in ecotoxicology.

Authors:  Marko Gerić; Goran Gajski; Vera Garaj-Vrhovac
Journal:  Ecotoxicol Environ Saf       Date:  2014-05-07       Impact factor: 6.291

6.  Quantitative detection of (125)IdU-induced DNA double-strand breaks with gamma-H2AX antibody.

Authors:  Olga A Sedelnikova; Emmy P Rogakou; Igor G Panyutin; William M Bonner
Journal:  Radiat Res       Date:  2002-10       Impact factor: 2.841

7.  Identification of genotoxic compounds using isogenic DNA repair deficient DT40 cell lines on a quantitative high throughput screening platform.

Authors:  Kana Nishihara; Ruili Huang; Jinghua Zhao; Sampada A Shahane; Kristine L Witt; Stephanie L Smith-Roe; Raymond R Tice; Shunichi Takeda; Menghang Xia
Journal:  Mutagenesis       Date:  2015-08-04       Impact factor: 3.000

Review 8.  GammaH2AX and cancer.

Authors:  William M Bonner; Christophe E Redon; Jennifer S Dickey; Asako J Nakamura; Olga A Sedelnikova; Stéphanie Solier; Yves Pommier
Journal:  Nat Rev Cancer       Date:  2008-11-13       Impact factor: 60.716
  8 in total
  2 in total

1.  The activated DNA double-strand break repair pathway in cumulus cells from aging patients may be used as a convincing predictor of poor outcomes after in vitro fertilization-embryo transfer treatment.

Authors:  Xu-Lei Sun; Hao Jiang; Dong-Xu Han; Yao Fu; Jian-Bo Liu; Yan Gao; Shu-Min Hu; Bao Yuan; Jia-Bao Zhang
Journal:  PLoS One       Date:  2018-09-20       Impact factor: 3.240

Review 2.  Application of In Vitro Metabolism Activation in High-Throughput Screening.

Authors:  Masato Ooka; Caitlin Lynch; Menghang Xia
Journal:  Int J Mol Sci       Date:  2020-10-31       Impact factor: 5.923
  2 in total

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