Literature DB >> 27518619

Mitochondrial Membrane Potential Assay.

Srilatha Sakamuru1, Matias S Attene-Ramos1, Menghang Xia2.   

Abstract

Mitochondrial function, a key indicator of cell health, can be assessed by monitoring changes in mitochondrial membrane potential (MMP). Cationic fluorescent dyes are commonly used tools to assess MMP. We used a water-soluble mitochondrial membrane potential indicator (m-MPI) to detect changes in MMP in HepG2 cells. A homogenous cell-based MMP assay was optimized and performed in a 1536-well plate format to screen several compound libraries for mitochondrial toxicity by evaluating the effects of chemical compounds on MMP.

Entities:  

Keywords:  1536-well plate format; Mesoxalonitrile 4-trifluoromethoxyphenylhydrazone (FCCP); Mitochondrial membrane potential (MMP); Mitochondrial membrane potential indicator (m-MPI); Mitochondrial toxicity

Mesh：

Substances：

Year:  2016        PMID： 27518619      PMCID： PMC5375165          DOI： 10.1007/978-1-4939-6346-1_2

Source DB:  PubMed          Journal:  Methods Mol Biol        ISSN： 1064-3745


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