| Literature DB >> 27508925 |
Vinod Kumar1, Jack R Brent2, Munish Shorie1, Harmanjit Kaur1, Gaganpreet Chadha1, Andrew G Thomas2, Edward A Lewis2, Aidan P Rooney2, Lan Nguyen2, Xiang Li Zhong2, M Grace Burke2, Sarah J Haigh2, Alex Walton3, Paul D McNaughter3, Aleksander A Tedstone3, Nicky Savjani3, Christopher A Muryn3, Paul O'Brien2,3, Ashok K Ganguli1,4, David J Lewis2,3, Priyanka Sabherwal1.
Abstract
We report the electrochemical detection of the redox active cardiac biomarker myoglobin (Mb) using aptamer-functionalized black phosphorus nanostructured electrodes by measuring direct electron transfer. The as-synthesized few-layer black phosphorus nanosheets have been functionalized with poly-l-lysine (PLL) to facilitate binding with generated anti-Mb DNA aptamers on nanostructured electrodes. This aptasensor platform has a record-low detection limit (∼0.524 pg mL(-1)) and sensitivity (36 μA pg(-1) mL cm(-2)) toward Mb with a dynamic response range from 1 pg mL(-1) to 16 μg mL(-1) for Mb in serum samples. This strategy opens up avenues to bedside technologies for multiplexed diagnosis of cardiovascular diseases in complex human samples.Entities:
Keywords: 2-D materials; aptamer; black phosphorus; heart disease; myoglobin; sensing
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Year: 2016 PMID： 27508925 DOI： 10.1021/acsami.6b06488
Source DB: PubMed Journal: ACS Appl Mater Interfaces ISSN： 1944-8244 Impact factor: 9.229