| Literature DB >> 27464757 |
Katarzyna Niemirowicz1, Bonita Durnaś2, Grażyna Tokajuk3, Katarzyna Głuszek2, Agnieszka Z Wilczewska4, Iwona Misztalewska4, Joanna Mystkowska5, Grzegorz Michalak1, Anna Sodo6, Marzena Wątek2, Bożena Kiziewicz7, Stanisław Góźdź2, Stanisław Głuszek8, Robert Bucki9.
Abstract
This study was designed to assess the antifungal/anti-biofilm and hemolytic properties of two polyene antibiotics, amphotericin B (AMF) and nystatin (NYS), attached to the surface of magnetic nanoparticles (MNP) against clinical isolates of Candida species and human red blood cells, respectively. The developed nanosystems, MNP@AMF and MNP@NYS, displayed stronger fungicidal activity than unbound AMF or NYS. Synergistic activity was observed with a combination of polyenes and MNPs against all tested Candida strains. Nanosystems were more potent than unbound agents when tested against Candida strains in the presence of pus, and as agents able to prevent Candida biofilm formation. The observed inactivation of catalase Cat1 in Candida cells upon treatment with the nanosystems suggests that disruption of the oxidation-reduction balance is a mechanism leading to inhibition of Candida growth. The significant decrease of polyenes lytic activity against host cells after their attachment to MNPs surface indicates improvement in their biocompatibility.Entities:
Keywords: Biofilm; Candida sp.; Fungal infections; Magnetic nanoparticles; Polyenes antibiotic
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Year: 2016 PMID： 27464757 DOI： 10.1016/j.nano.2016.07.006
Source DB: PubMed Journal: Nanomedicine ISSN： 1549-9634 Impact factor: 5.307