Ghobad Mohammadi1,2, Amineh Shakeri3, Ali Fattahi4, Pardis Mohammadi4, Ali Mikaeili5, Alireza Aliabadi6, Khosro Adibkia7. 1. Novel Drug Delivery Research Center, Faculty of Pharmacy, Kermanshah University of Medical Sciences,, Kermanshah, Iran. ghobadmohammadi@yahoo.com. 2. Faculty of Pharmacy, Kermanshah University of Medical Sciences, Kermanshah, Iran. ghobadmohammadi@yahoo.com. 3. Student Research Committee, Kermanshah University of Medical Sciences,, Kermanshah, Iran. 4. Novel Drug Delivery Research Center, Faculty of Pharmacy, Kermanshah University of Medical Sciences,, Kermanshah, Iran. 5. Faculty of Medicine, Kermanshah University of Medical Sciences, Kermanshah, Iran. 6. Department of Medicinal Chemistry, Faculty of Pharmacy, Kermanshah University of Medical Sciences,, Kermanshah, Iran. 7. Faculty of Pharmacy, Tabriz University of Medical Sciences,, Tabriz, Iran.
Abstract
PURPOSE: Nystatin loaded PLGA and PLGA-Glucosamine nanoparticles were formulated. PLGA were functionalized with Glucosamine (PLGA-GlcN) to enhance the adhesion of nanoparticles to Candida Albicans (C.albicans) cell walls. METHOD: Quasi-emulsion solvent diffusion method was employed using PLGA and PLGA-GlcN with various drug-polymer ratios for the preparation of nanoparticles. The nanoparticles were evaluated for size, zeta potential, polydispersity index, drug crystallinity, loading efficiency and release properties. DSC, SEM, XRPD, 1H-NMR, and FT-IR were performed to analyze the physicochemical properties of the nanoparticles. Antifungal activity of the nanoparticles was evaluated by determination of MICs against C.albicans. RESULTS: The spectra of 1H-NMR and FT-IR analysis ensured GlcN functionalization on PLGA nanoparticles. SEM characterization confirmed that particles were in the nanosize range and the particle size for PLGA and PLGA-GlcN nanoparticles were in the range of 108.63 ± 4.5 to 168.8 ± 5.65 nm and 208.76 ± 16.85 nm, respectively. DSC and XRPD analysis ensured reduction of the drug crystallinity in the nanoparticles. PLGA-GlcN nanoparticles exhibit higher antifungal activity than PLGA nanoparticles. CONCLUSION: PLGA-GlcN nanoparticles showed more antifungal activity with appropriate physicochemical properties than pure Nystatin and PLGA nanoparticles.
PURPOSE:Nystatin loaded PLGA and PLGA-Glucosamine nanoparticles were formulated. PLGA were functionalized with Glucosamine (PLGA-GlcN) to enhance the adhesion of nanoparticles to Candida Albicans (C.albicans) cell walls. METHOD: Quasi-emulsion solvent diffusion method was employed using PLGA and PLGA-GlcN with various drug-polymer ratios for the preparation of nanoparticles. The nanoparticles were evaluated for size, zeta potential, polydispersity index, drug crystallinity, loading efficiency and release properties. DSC, SEM, XRPD, 1H-NMR, and FT-IR were performed to analyze the physicochemical properties of the nanoparticles. Antifungal activity of the nanoparticles was evaluated by determination of MICs against C.albicans. RESULTS: The spectra of 1H-NMR and FT-IR analysis ensured GlcN functionalization on PLGA nanoparticles. SEM characterization confirmed that particles were in the nanosize range and the particle size for PLGA and PLGA-GlcN nanoparticles were in the range of 108.63 ± 4.5 to 168.8 ± 5.65 nm and 208.76 ± 16.85 nm, respectively. DSC and XRPD analysis ensured reduction of the drug crystallinity in the nanoparticles. PLGA-GlcN nanoparticles exhibit higher antifungal activity than PLGA nanoparticles. CONCLUSION: PLGA-GlcN nanoparticles showed more antifungal activity with appropriate physicochemical properties than pure Nystatin and PLGA nanoparticles.
Authors: M A Pfaller; D J Diekema; M Mendez; C Kibbler; P Erzsebet; S-C Chang; D L Gibbs; V A Newell Journal: J Clin Microbiol Date: 2006-10 Impact factor: 5.948
Authors: Katarzyna Niemirowicz; Bonita Durnaś; Grażyna Tokajuk; Katarzyna Głuszek; Agnieszka Z Wilczewska; Iwona Misztalewska; Joanna Mystkowska; Grzegorz Michalak; Anna Sodo; Marzena Wątek; Bożena Kiziewicz; Stanisław Góźdź; Stanisław Głuszek; Robert Bucki Journal: Nanomedicine Date: 2016-07-25 Impact factor: 5.307