| Literature DB >> 27423270 |
Ankita Agarwal1, Ajay Kumar Sharma2, D Sukumaran2, Manmohan Parida1, Paban Kumar Dash3.
Abstract
Expansion of CHIKV outbreaks with appearance of novel mutations are reported from many parts of the world. Two novel mutations viz. E1:K211E and E2:V264A in background of E1:226A are recently identified from Aedes aegypti dominated areas of India. In this study, the role of these mutations in modulation of infectivity, dissemination and transmission by two different Aedes species was studied. Mutations were sequentially constructed in CHIKV genome and female Ae. aegypti and Aedes albopictus mosquitoes were orally infected with eight different CHIKV mutants. Double mutant virus containing E1:K211E and E2:V264A mutations in background of E1:226A revealed remarkably higher fitness for Ae. aegypti, as indicated by significant increase in virus infectivity (13 fold), dissemination (15 fold) and transmission (62 fold) compared to parental E1:226A virus. These results indicate that adaptive mutations in CHIKV are leading to efficient CHIKV circulation in Ae. aegypti endemic areas, contributing and sustaining the major CHIKV outbreaks.Entities:
Keywords: Aedes; Chikungunya; Dissemination; Infection; Mutations; Transmission
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Year: 2016 PMID： 27423270 DOI： 10.1016/j.virol.2016.06.025
Source DB: PubMed Journal: Virology ISSN： 0042-6822 Impact factor: 3.616