Literature DB >> 2742125

A continuous-flow automated assay for iodometric estimation of hydroperoxides.

S M Thomas1, W Jessup, J M Gebicki, R T Dean.   

Abstract

An iodometric method for the analysis of hydroperoxides has been automated to allow analysis of aqueous biological samples (containing less than 20 mg/ml protein) and lipid hydroperoxide extracts. The evolution of triiodide ions is measured spectrophotometrically at 360 nm. Dependent on the type of sample, 30-60 samples can be analyzed per hour and the system allows detection of less than 100 pmol of peroxide. The assay is linear over a range of 100 pmol to 25 nmol. The sample volume used routinely was 80 microliters.

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Year:  1989        PMID： 2742125     DOI： 10.1016/0003-2697(89)90322-9

Source DB:  PubMed          Journal:  Anal Biochem        ISSN： 0003-2697            Impact factor:   3.365


  9 in total

Review 1.  Biomarkers of exposure to vitamins A, C, and E and their relation to lipid and protein oxidation markers.

Authors:  Lars O Dragsted
Journal:  Eur J Nutr       Date:  2008-05       Impact factor: 5.614

Review 2.  Oxidation as an important factor of protein damage: Implications for Maillard reaction.

Authors:  L Trnkova; J Drsata; I Bousova
Journal:  J Biosci       Date:  2015-06       Impact factor: 1.826

3.  Time-course studies by synchrotron X-ray solution scattering of the structure of human low-density lipoprotein during Cu(2+)-induced oxidation in relation to changes in lipid composition.

Authors:  D F Meyer; A S Nealis; C H Macphee; P H Groot; K E Suckling; K R Bruckdorfer; S J Perkins
Journal:  Biochem J       Date:  1996-10-01       Impact factor: 3.857

4.  Long-lived reactive species on free-radical-damaged proteins.

Authors:  J A Simpson; S Narita; S Gieseg; S Gebicki; J M Gebicki; R T Dean
Journal:  Biochem J       Date:  1992-03-15       Impact factor: 3.857

5.  The action of defined oxygen-centred free radicals on human low-density lipoprotein.

Authors:  S Bedwell; R T Dean; W Jessup
Journal:  Biochem J       Date:  1989-09-15       Impact factor: 3.857

6.  Formation of peroxides in amino acids and proteins exposed to oxygen free radicals.

Authors:  S Gebicki; J M Gebicki
Journal:  Biochem J       Date:  1993-02-01       Impact factor: 3.857

7.  Protein hydroperoxides can give rise to reactive free radicals.

Authors:  M J Davies; S Fu; R T Dean
Journal:  Biochem J       Date:  1995-01-15       Impact factor: 3.857

8.  Alpha-tocopherol consumption during low-density-lipoprotein oxidation.

Authors:  W Jessup; S M Rankin; C V De Whalley; J R Hoult; J Scott; D S Leake
Journal:  Biochem J       Date:  1990-01-15       Impact factor: 3.857

9.  Low-density lipoprotein is the major carrier of lipid hydroperoxides in plasma. Relevance to determination of total plasma lipid hydroperoxide concentrations.

Authors:  J Nourooz-Zadeh; J Tajaddini-Sarmadi; K L Ling; S P Wolff
Journal:  Biochem J       Date:  1996-02-01       Impact factor: 3.857
  9 in total

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