Literature DB >> 27417716

Using the omega leader sequence of tobacco mosaic virus to transform tomato fruits with the papillomavirus hpv16 L1 gene to enhance production of the antigenic protein HPV16 L11.

R K Salyaev1, N I Rekoslavskaya2, A S Stolbikov2,3, A V Tret'yakova2,3.   

Abstract

To enhance translation of the L1 protein antigen of the oncogenic human papillomavirus type HPV16 L1, the Ω sequence of the 5'-untranslated region of tobacco mosaic virus was inserted into a genetic construct as an enhancer. To transform plants, A. tumefaciens EHA105 cells were transfected with this construct. After the genetic transformation, the HPV16 L1 protein antigen was detected in tomato fruit in amounts of 287-2330 ng per 1 mg total soluble protein, which significantly exceeds the amount of the protein antigen obtained in our previous studies without using the omega leader sequence.

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Year:  2016        PMID： 27417716     DOI： 10.1134/S1607672916030078

Source DB:  PubMed          Journal:  Dokl Biochem Biophys        ISSN： 1607-6729            Impact factor:   0.788


  6 in total

1.  Study of the mucosal immune response duration in mice after administration of a candidate edible vaccine based on transgenic tomato plants carrying the TBI-HBS gene.

Authors:  R K Salyaev; N I Rekoslavskaya; S N Shchelkunov; A S Stolbikov; R V Hammond
Journal:  Dokl Biochem Biophys       Date:  2009 Sep-Oct       Impact factor: 0.788

Review 2.  Human papillomavirus vaccines in plants.

Authors:  Colomba Giorgi; Rosella Franconi; Edward P Rybicki
Journal:  Expert Rev Vaccines       Date:  2010-08       Impact factor: 5.217

3.  Candidate mucosal vaccine against hepatitis B based on tomatoes transgenic for the preS2-S gene.

Authors:  R K Salyaev; N I Rekoslavskaya; A S Stolbikov; A V Tret'yakova
Journal:  Dokl Biochem Biophys       Date:  2012-11-07       Impact factor: 0.788

4.  Insight into the structural organization of the omega leader of TMV RNA: the role of various regions of the sequence in the formation of a compact structure of the omega RNA.

Authors:  Sultan C Agalarov; Evgeny A Sogorin; Nikolay E Shirokikh; Alexander S Spirin
Journal:  Biochem Biophys Res Commun       Date:  2010-11-27       Impact factor: 3.575

5.  An E7-based therapeutic vaccine protects mice against HPV16 associated cancer.

Authors:  Aldo Venuti; Silvia Massa; Vadim Mett; Laura Dalla Vedova; Francesca Paolini; Rosella Franconi; Vidadi Yusibov
Journal:  Vaccine       Date:  2009-02-05       Impact factor: 3.641

6.  Human papillomavirus vaccination: recommendations of the Advisory Committee on Immunization Practices (ACIP).

Authors:  Lauri E Markowitz; Eileen F Dunne; Mona Saraiya; Harrell W Chesson; C Robinette Curtis; Julianne Gee; Joseph A Bocchini; Elizabeth R Unger
Journal:  MMWR Recomm Rep       Date:  2014-08-29
  6 in total
  3 in total

1.  Synthesis of Proteins Encoded by the Early Genes E2, E6, and E7 of Papillomavirus of Type 16 in the Plant Expression System.

Authors:  R K Salyaev; N I Rekoslavskaya; A S Stolbikov
Journal:  Dokl Biochem Biophys       Date:  2018-11-05       Impact factor: 0.788

2.  The study of immunogenicity of the antigenic protein of high risk oncogenic type of the human papillomavirus HPV16 L1 produced in the plant expression system on the base of transgenic tomato.

Authors:  R K Salyaev; N I Rekoslavskaya; A S Stolbikov; A V Tretyakova
Journal:  Dokl Biochem Biophys       Date:  2017-07-20       Impact factor: 0.788

3.  Expression of Human Immunodeficiency Virus type 1 (HIV-1) coat protein genes in plants using cotton leaf curl Multan betasatellite-based vector.

Authors:  Elham Ataie Kachoie; Seyed Ali Akbar Behjatnia; Sara Kharazmi
Journal:  PLoS One       Date:  2018-01-05       Impact factor: 3.240
  3 in total

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