A randomized phase III study of carfilzomib vs low-dose corticosteroids with optional cyclophosphamide in relapsed and refractory multiple myeloma (FOCUS).

This randomized, phase III, open-label, multicenter study compared carfilzomib monotherapy against low-dose corticosteroids and optional cyclophosphamide in relapsed and refractory multiple myeloma (RRMM). Relapsed and refractory multiple myeloma patients were randomized (1:1) to receive carfilzomib (10-min intravenous infusion; 20 mg/m2 on days 1 and 2 of cycle 1; 27 mg/m2 thereafter) or a control regimen of low-dose corticosteroids (84 mg of dexamethasone or equivalent corticosteroid) with optional cyclophosphamide (1400 mg) for 28-day cycles. The primary endpoint was overall survival (OS). Three-hundred and fifteen patients were randomized to carfilzomib (n=157) or control (n=158). Both groups had a median of five prior regimens. In the control group, 95% of patients received cyclophosphamide. Median OS was 10.2 (95% confidence interval (CI) 8.4-14.4) vs 10.0 months (95% CI 7.7-12.0) with carfilzomib vs control (hazard ratio=0.975; 95% CI 0.760-1.249; P=0.4172). Progression-free survival was similar between groups; overall response rate was higher with carfilzomib (19.1 vs 11.4%). The most common grade ⩾3 adverse events were anemia (25.5 vs 30.7%), thrombocytopenia (24.2 vs 22.2%) and neutropenia (7.6 vs 12.4%) with carfilzomib vs control. Median OS for single-agent carfilzomib was similar to that for an active doublet control regimen in heavily pretreated RRMM patients.

RCT Entities:   Population Interventions Outcomes

Introduction

Therapeutic options are limited for patients with relapsed and refractory multiple myeloma (RRMM). Although treatment may induce remission, patients experience multiple relapses.[1] Each subsequent remission becomes increasingly shorter in duration, and patients ultimately die from complications of the disease.[2, 3, 4, 5] Historically, patients have survival duration of <12 months and response rates of approximately 15% at fourth relapse.[3, 4, 6, 7]

Treatment options for patients refractory to both the proteasome inhibitor bortezomib and an immunomodulatory agent are especially limited. In a retrospective study of 286 patients who were refractory to both bortezomib and immunomodulatory agents, median overall survival (OS) was approximately 9 months.[3]

Carfilzomib and pomalidomide are two treatments that are currently approved in the United States for patients with RRMM who have received prior bortezomib and either thalidomide or lenalidomide.[8, 9] The approval of carfilzomib, a proteasome inhibitor that binds selectively and irreversibly to its target, was based on the overall response rate (ORR) from a phase II study in heavily pretreated patients.[10] That study and other phase II studies further established the safety profile of single-agent carfilzomib.[10, 11, 12, 13, 14, 15]

Here we present the results of the randomized phase III study PX-171-011 (FOCUS), which investigated single-agent carfilzomib vs low-dose corticosteroids with optional cyclophosphamide in patients with advanced RRMM. In the absence of an established standard of care, the control regimen was chosen on the basis of discussions with multiple myeloma (MM) experts and results from several small studies (20–42 patients) that showed disease response and symptom abatement using this combination as salvage therapy in advanced MM.[16, 17, 18]

Methods

Participants

FOCUS was a randomized, open-label, phase III study. Participants were recruited from 77 study sites in Europe, Australia, New Zealand and South Korea.

Eligible patients (age ⩾18 years) with Eastern Cooperative Oncology Group performance status 0–2 had received at least three prior treatments for MM, including bortezomib, lenalidomide or thalidomide, an alkylating agent, corticosteroids and anthracycline (for patients enrolled prior to Amendment 2), and were refractory to their most recent therapy. Patients with some impairment of bone marrow and organ function were eligible, including those with reduced platelet counts (>30 000/μl) and with creatinine clearance (CrCl) ⩾15 ml/min.

Exclusion criteria included the presence of Waldenström macroglobulinemia, immunoglobulin M myeloma, plasma cell leukemia, other malignancy in the previous 3 years, POEMS syndrome, prior carfilzomib, major surgery within 21 days prior to randomization, New York Heart Association class III/IV congestive heart failure, myocardial infarction in the previous 3 months, HIV seropositivity, active hepatitis, and significant neuropathy (grade 3 or 4, or grade 2 with pain).

The protocol was approved by each study center's Institutional Review Board or Independent Ethics Committee. The study was conducted in accordance with the Declaration of Helsinki and was consistent with the Guidelines for Good Clinical Practice and all applicable regulatory guidelines. All patients provided written informed consent.

Procedures

Patients were randomized 1:1 using an interactive Web response system to receive either carfilzomib or control (defined as corticosteroids with optional cyclophosphamide) and were stratified by number of prior therapies (3 vs 4 vs ⩾5) and geographic region (Europe vs non-Europe). The randomization schedule was prepared using a blocked randomization scheme (block size of 4). Study investigators and patients were not blinded to treatment. The sponsor's study team did not evaluate unblinded aggregate study results until completion of the study. Unmasked safety data were evaluated by an independent data-monitoring committee via an independent external statistical analysis group on a regular basis.

Patients were treated in 28-day cycles according to the following schedule: during cycles 1–9, patients in the carfilzomib group received carfilzomib at a starting dose of 20 mg/m2 administered intravenously over 10 min on days 1 and 2 of cycle 1, which was escalated to a target dose of 27 mg/m2 on days 8, 9, 15 and 16 of cycle 1 and continued on days 1, 2, 8, 9, 15 and 16 of cycles 2–9. During cycles 10 and beyond, carfilzomib was administered at 27 mg/m2 on days 1, 2, 15 and 16; treatment on days 8 and 9 was optional per the investigator's discretion. During cycle 1, patients received oral (PO) and intravenous (IV) hydration (before and after dose), dexamethasone 4 mg (before dose; PO or IV), and ciprofloxacin (500 mg PO, once daily (QD)). For patients with a history of herpes zoster, valacyclovir or an equivalent antiviral medication was required.

For patients randomized to receive control, treatment consisted of a corticosteroid (prednisone 30 mg PO every other day, dexamethasone 6 mg PO every other day, or other equivalent corticosteroid (not to exceed 84 mg dexamethasone or equivalent per 28-day cycle)). Patients could receive optional cyclophosphamide 50 mg PO, QD (1400 mg maximum per cycle) per the investigator's discretion.

Treatment was continued until confirmed progressive disease, unacceptable toxicity or consent withdrawal. Dose reductions of carfilzomib to 20, 15 or 11 mg/m2 were permitted, as necessary, to manage toxicity.

Objectives

The primary endpoint of the study was OS, which was defined as the time from randomization to death from any cause. Secondary endpoints included progression-free survival (PFS), which was defined as the time from randomization to confirmed progressive disease or death from any cause; ORR, which was defined as the proportion of patients who achieved partial response or better according to the International Myeloma Working Group criteria;[19] duration of response, which was defined as the time from the start date of achieving a partial response or better until confirmed progressive disease or death from any cause; clinical benefit rate, which was defined as ORR with the addition of minimal response according to European Group for Blood and Marrow Transplant criteria;[20] disease control rate, which was defined as clinical benefit rate plus stable disease lasting ⩾8 weeks; and safety.

Assessments

Patients were evaluated for disease response and progression (as determined by investigator assessment) on day 1 of each cycle. Treatment-emergent adverse events were assessed at each visit according to the National Cancer Institute Common Terminology Criteria for Adverse Events v4.0 and were coded using the Medical Dictionary for Regulatory Activities v15.1. Treatment-emergent adverse events were defined as adverse events (AEs) that started on or after the first administration of study treatment and within 30 days of the last administration of study treatment. All available laboratory data were reported according to International System of Units. Results of selected laboratory analyses were summarized descriptively and graded according to National Cancer Institute Common Terminology Criteria for Adverse Events, v4.0. An independent data-monitoring committee reviewed the study data on an ongoing basis. A preplanned interim efficacy analysis took place after approximately 75% of the OS events had occurred, and the independent data-monitoring committee recommended that the study be continued until enough events had occurred to perform the final analysis.

Statistical analyses

The study had a planned enrollment of 302 patients; it was estimated that 253 OS events would provide 80% power to detect a 30% reduction in the risk of death (that is, hazard ratio (HR) of 0.70) with an overall one-sided alpha of 0.025 for carfilzomib compared with control, if one interim and final analysis were planned. The interim analysis was scheduled to occur after approximately 75% of the planned total OS events were reported. An O'Brien-Fleming-type alpha-spending function determined the monitoring boundaries for early stopping for efficacy so that the overall Type I error was less than or equal to 0.025 (one-sided). The significance level was adjusted per actual number of events observed using a Lan-DeMets implementation of the O'Brien-Fleming group-sequential procedure to account for multiple analyses of the primary endpoint (one interim analysis and one final analysis).

The assumptions for the performance of the control arm were estimated from available information from retrospective analyses, as there were no randomized trial results in advanced RRMM, and were further supported by review publications that estimated a median OS of approximately 6 months for patients with multiply relapsed MM.[6, 7] The assumption for the carfilzomib group (median OS of approximately 8.6 months) was derived from phase II results (median OS of 15.4 months),[10] while adjusting for the more advanced disease characteristics expected in the FOCUS study compared with previous phase II trials.

All efficacy analyses were based on the intent-to-treat population, which consisted of all randomized patients. A stratified log-rank test was used as primary inference to compare time to event outcomes such as OS and PFS between the two treatment groups. One-sided P-values for OS and PFS were from the stratified log-rank test with number of previous therapies (3 vs 4 vs ⩾5) and geographical region (Europe vs non-Europe) as stratification factors. The distribution of OS and PFS were summarized using the Kaplan–Meier method per arm. Hazard ratios and corresponding 95% confidence intervals (CIs) were calculated using a stratified Cox proportional hazards model. One-sided P-values for ORR, clinical benefit rate and disease control rate were from the Cochran–Mantel–Haenszel chi-squared test using the same randomization stratification factors as above. Adverse events were summarized using descriptive statistics. Relative dose intensity was calculated as the actual dose intensity divided by the planned dose intensity.

This study is registered with Clinicaltrials.gov, identifier NCT01302392.

Changes in methods after trial commencement

In order to adhere to guidance from the Committee for Medicinal Products for Human Use to design a study with survival as the primary endpoint, the primary endpoint was changed from PFS to OS. This was accompanied by an increase in sample size from 84 to 302 in order to provide 80% power to detect a 30% reduction in the risk of death for carfilzomib over control. A regional stratification variable was added to control for the additional sites and countries that were added for the increased sample size. Since OS became the primary endpoint, investigator's assessment of response and progression were used to determine the respective secondary efficacy endpoints, instead of the Independent Review Committee.

To align with International Myeloma Working Group guidelines, patients with minimal response or stable disease lasting at least 8 weeks were considered to have achieved disease control (originally 6 weeks).

An inclusion criterion that required patients to have received prior treatment with an anthracycline was removed in order to expand study access to patients being managed per current standard of care (which does not support the routine use of anthracyclines). An exclusion criterion that excluded patients with any contraindications to required concomitant drugs or supportive treatments was added to ensure patient safety.

Results

Patients and enrollment

From September 2010 to October 2012, 403 patients from 77 study sites in Europe, Australia, New Zealand and South Korea were screened for eligibility. A total of 315 patients were randomized into the study and comprised the intent-to-treat population (Figure 1); 157 patients were randomized to the carfilzomib group and 158 were randomized to the control group. The safety population included the 310 patients who received at least one dose of study drug (carfilzomib, n=157; control, n=153).

Figure 1

Random assignment and follow-up.

The required number of events to conduct the final efficacy analysis per protocol was reached on 10 July 2014 and this is the data cutoff date used for this analysis. At data cutoff, six patients in each of the carfilzomib and control groups were alive and receiving study treatment. Median follow-up time for OS was 27.8 months (95% CI 24.6–33.7) and 29.8 months (95% CI 24.3–33.6) in the carfilzomib and control groups, respectively.

Baseline characteristics were generally well-balanced between treatment groups, with the exception being that more patients in the carfilzomib group had urine protein electrophoresis-measurable only disease (17 vs 9%) and light-chain proteinuria (18 vs 10%) only (Table 1). The overall study population had poor prognostic characteristics, including International Staging System stage III and Eastern Cooperative Oncology Group performance status ⩾2 in 39 and 20% of patients, respectively. Patients received a median of five prior regimens of therapy (range, 3 to 17) and a median of eight prior antimyeloma agents (range, 4 to 17). Sixty-three percent of patients were refractory to both bortezomib and an immunomodulatory agent (Table 2).

Table 1

Baseline patient and disease characteristics

Characteristic	Carfilzomib n=157	Control n=158	Total N=315
Median age, years (range)	63 (32–85)	66 (43–81)	65 (32–85)
⩾65 years, n (%)	75 (48)	89 (56)	164 (52)
⩾75 years, n (%)	25 (16)	24 (15)	49 (16)
Median time since diagnosis, years (range)	6 (2–20)	5 (2–24)	6 (2–24)
Males, n (%)	82 (52)	96 (61)	178 (57)
Region (%)
Europe	140 (89)	138 (87)	278 (88)
Race, n (%)
White	151 (96)	148 (94)	299 (95)
Black	1 (1)	1 (1)	2 (1)
Other	5 (3)	9 (6)	14 (4)
Ethnicity, n (%)
Not Hispanic or Latino	131 (83)	129 (82)	260 (83)
Not reported	4 (3)	5 (3)	9 (3)
ISS stage at initial diagnosis, n (%)
I	26 (17)	20 (13)	46 (15)
II	32 (20)	41 (26)	73 (23)
III	66 (42)	56 (35)	122 (39)
Unknown	33 (21)	41 (26)	74 (23)
Cytogenetics by FISH, n (%)
High riska	22 (14)	29 (18)	51 (16)
Standard riskb	68 (43)	76 (48)	144 (46)
Unknown risk	67 (43)	53 (34)	120 (38)
ECOG performance status, n (%)
0	49 (31)	31 (20)	80 (25)
1	78 (50)	93 (59)	171 (54)
⩾2	30 (19)	34 (22)	64 (20)
Serum β2-microglobulin ⩾5.5 mg/l, n (%)	76 (48)	82 (52)	158 (50)
Presence of bone lesions, n (%)	105 (67)	111 (70)	216 (69)
Light-chain immunoglobulin, n (%)
Kappa	93 (59)	95 (60)	188 (60)
Lambda	64 (41)	62 (39)	126 (40)
Missing	0 (0)	1 (1)	1 (<1)
Measurable disease category, n (%)
SPEP and UPEP	57 (36)	51 (32)	108 (34)
SPEP only	72 (46)	91 (58)	163 (52)
UPEP only	27 (17)	14 (9)	41 (13)
Light chain disease only	29 (18)	16 (10)	45 (14)
Median CrCl (range), ml/min	65 (16−190)	63 (14−207)	64 (14−207)
CrCl <30 ml/min, n (%)	17 (11)	14 (9)	31 (10)
Baseline neuropathy grade, n (%)
0	76 (48)	81 (51)	157 (50)
1	66 (42)	56 (35)	122 (39)
⩾2	15 (10)	21 (13)	36 (11)

Abbreviations: CrCl, creatinine clearance; ECOG, Eastern Cooperative Oncology Group; FISH, fluorescence in situ hybridization; ISS, International staging system; SPEP, serum protein electrophoresis; UPEP, urine protein electrophoresis.

Includes patients with genetic subtype t(4;14) or t(14;16) or with deletion 17p in 60% or more of plasma cells, according to central review of bone marrow samples obtained at study entry.

Includes patients without t(4;14) or t(14;16) and with deletion 17p in fewer than 60% of plasma cells.

Table 2

Prior regimens received by patients

	Carfilzomib n=157	Control n=158	Total N=315
Number of prior regimens, n (%)
Median (range)	5 (3–15)	5 (3–17)	5 (3–17)
3	17 (11)	19 (12)	36 (11)
4	34 (22)	35 (22)	69 (22)
5	39 (25)	37 (23)	76 (24)
⩾6	67 (43)	67 (42)	134 (43)
Median number of unique prior antimyeloma drugs (range)	8 (5–17)	8.5 (4–14)	8 (4–17)
Patients who had prior transplant, n (%)	107 (68)	102 (65)	209 (66)
Refractory to most recent therapy, n (%)	157 (100)	157 (99)	314 (100)
Nonresponsive (<MR)	112 (71)	112 (71)	224 (71)
Progression during therapy	82 (52)	83 (53)	165 (52)
Progression within 60 days of completion of therapy	34 (22)	39 (25)	73 (23)
Prior therapy received, n (%)
Bortezomib, IMiD, alkylating agent, corticosteroid	157 (100)	158 (100)	315 (100)
Lenalidomide	132 (84)	125 (79)	257 (82)
Thalidomide	116 (74)	124 (78)	240 (76)
Pomalidomide	12 (8)	8 (5)	20 (6)
Anthracyclines	117 (75)	122 (77)	239 (76)
Received in last prior regimen, n (%)
Bortezomib	36 (23)	41 (26)	77 (24)
Bortezomib refractory in last prior regimen	36 (23)	41 (26)	77 (24)
Refractory to (in any prior regimen), n (%)
Bortezomib	103 (66)	108 (68)	211 (67)
Lenalidomide	114 (73)	112 (71)	226 (72)
Thalidomide	84 (54)	94 (59)	178 (57)
Bortezomib and IMiD	97 (62)	100 (63)	197 (63)
Alkylating agents	136 (87)	133 (84)	269 (85)

Abbreviations: IMiD, immunomodulatory agent; MR, minimal response.

Patients may be counted in more than one category.

Efficacy

Median treatment duration was 16.3 weeks (range, 0.3 to 138.4) in the carfilzomib group and 10.7 weeks (range, 0.4 to 138.3) in the control group. Ninety-five percent of patients in the control group received cyclophosphamide in addition to corticosteroids. Relative carfilzomib and corticosteroid dose intensity was 99.9% in both the carfilzomib group and the control group.

The data met the assumptions of the statistical tests used. The similarity of variances between treatment groups for time-to-event outcomes was not compared as the variability of the per-arm estimate (for example, median) depends on the number of events, which is determined by efficacy.

The HR for OS in the carfilzomib group compared with the control group was 0.975 (95% CI 0.760–1.249; one-sided P=0.4172). As the study did not meet the primary objective of carfilzomib superiority over control, based on the hierarchical testing procedure to control multiplicity, all the P-values for secondary endpoints were multiplicity unadjusted. Median OS was 10.2 months (95% CI 8.4–14.4) with carfilzomib compared with 10.0 months (95% CI 7.7–12.0) with control (Figure 2). OS rates at 12 and 18 months were 48 and 34% in the carfilzomib group vs 42 and 30% in the control group, respectively (Table 3).

Figure 2

Kaplan−Meier estimates of (a) OS and (b) PFS.

Table 3

Efficacy results

	Carfilzomib n=157	Control n=158	One-sided P-valuea
Median OS, months (range; 95% CI)	10.2 (8.4–14.4)	10.0 (7.7–12.0)	0.4172
Hazard ratio (carfilzomib/control) (95% CI)	0.975 (0.760–1.249)
Median PFS, years (95% CI)	3.7 (2.8–4.2)	3.3 (2.2–5.2)	0.2479
Hazard ratio (carfilzomib/control) (95% CI)	1.091 (0.843–1.410)
Best overall responseb, n (%)
CR	1 (1)	0 (0)	NA
VGPR	5 (3)	5 (3)	NA
PR	24 (15)	13 (8)	NA
MR	19 (12)	15 (9)	NA
SD	70 (45)	74 (47)	NA
PD	26 (17)	35 (22)	NA
Unable to evaluate	12 (8)	16 (10)	NA
ORR, n (%)	30 (19)	18 (11)	NA
95% CIc of ORR	13–26	7–17	0.0305
Median time to overall response, monthsd (range)	2.0 (0.5–11.0)	3.9 (1.0–28.0)	NA
Median duration of overall response, months (95% CI)	7.2 (4.6–12.0)	9.5 (3.7–NE)	NA
CBR, n (%)	49 (31)	33 (21)	NA
95% CIc of CBR	24–39	15–28	0.0212
Median time to clinical benefit response, monthsd (range)	1.5 (0.4–5.0)	1.9 (0.9–17.0)	NA
Median duration of clinical benefit response, months (95% CI)	6.4 (4.9–8.3)	8.3 (6.5–12.9)	NA
DCR, n (%)	119 (76)	107 (68)	NA
95% CIc of DCR	68–82	60–75	0.0514
Median duration of disease control, months (95% CI)	5.5 (3.9–6.5)	6.6 (5.4–8.8)	NA

Abbreviations: CBR, clinical benefit rate; CI, confidence interval; CR, complete response; DCR, disease control rate; MR, minimal response; NA, not applicable; NE, not estimable; ORR, overall response rate; OS, overall survival; PD, progressive disease; PFS, progression-free survival; PR, partial response; sCR, stringent complete response; SD, stable disease; VGPR, very good partial response.

Overall response rate=sCR, CR, VGPR, or PR; clinical benefit response rate=sCR, CR, VGPR, PR, or MR; disease control=sCR, CR, VGPR, PR, MR, or SD.

One-sided P-values for OS and PFS were from stratified log-rank test with number of previous therapies (3 vs 4 vs ⩾5) and geographical region (Europe vs non-Europe) as stratification factors; one-sided P-values for ORR, CBR and DCR were from Cochran–Mantel–Haenszel chi-squared test with number of previous therapies (3 vs 4 vs⩾5) and geographical region (Europe vs non-Europe) as stratification factors; all P-values were unadjusted.

Best overall response was defined as a patient's best response during the study. A confirmed MR required a minimum duration of 8 weeks in addition to at least two consecutive assessment results of MR or higher. A confirmed SD required a minimum duration of 8 weeks.

Clopper–Pearson interval.

Medians, percentiles and their 95% CIs were estimated using the Kaplan−Meier method.

Median PFS was 3.7 months (95% CI 2.8–4.2) in the carfilzomib group compared with 3.3 months (95% CI 2.2–5.2) in the control group (HR 1.091; 95% CI 0.843–1.410; one-sided P=0.2479) (Figure 2). PFS rates at 12 and 18 months were 14 and 7% with carfilzomib vs 17 and 12% with control, respectively (Table 3).

In the carfilzomib group, 66.9% of patients received any subsequent antimyeloma therapy compared with 62.0% of patients in the control group (Supplementary Table 1). A trend was observed of patients in the control group starting next antimyeloma therapy sooner than in the carfilzomib group, which led to more patients in the control group being censored for PFS. An exploratory analysis of PFS that treated the receipt of next antimyeloma therapy as a PFS event was consistent with the primary PFS analysis (Supplementary Table 2).

The ORR was higher in the carfilzomib group compared with the control group (19.1 vs 11.4%, respectively; odds ratio, 1.84; 95% CI 0.97–3.49; one-sided P=0.0305) (Table 3). A trend was observed for higher ORRs in the carfilzomib group among patients sensitive to bortezomib, refractory to bortezomib, or refractory to both bortezomib and an immunomodulatory agent (Supplementary Table 3). For all patients achieving at least partial response, the median duration of response was 7.2 months (95% CI 4.6–12.0) in the carfilzomib and 9.5 months (95% CI 3.7—not estimable) in the control group. The clinical benefit rate was 31.2 vs 20.9% in the carfilzomib vs control groups (odds ratio, 1.70; 95% CI 1.02–2.85; one-sided P=0.0212), and the disease control rate was 75.8 vs 67.7%, respectively.

Safety

Incidence rates of any-grade treatment-emergent adverse events were similar between the carfilzomib (98% of patients) and control (94%) groups (Table 4). Serious AEs (carfilzomib group, 59% control group, 51%) and grade 5 AEs (carfilzomib group, 19% control group, 22%) occurred at similar rates in both groups. Deaths due to AEs occurred in 10% of patients in the carfilzomib group compared with 12% in the control group (Supplementary Table 4). In the carfilzomib group, 5% of patients had their dose reduced at least once due to an AE, 1% had their dose interrupted due to an AE, 13% had their dose delayed due to an AE. Discontinuation due to an AE occurred in 15% of patients in the carfilzomib group and 20% of patients in the control group.

Table 4

Hematologic and nonhematologic adverse eventsa

Adverse event preferred term, n (%)	Carfilzomib (n=157)		Control (n=153)
	All grades	Grade ⩾3	All grades	Grade ⩾3
Any adverse event	154 (98)	118 (75)	143 (94)	109 (71)
Hematologic
Anemia	88 (56)	40 (25)	75 (49)	47 (31)
Thrombocytopenia	59 (38)	38 (24)	46 (30)	34 (22)
Neutropenia	23 (15)	12 (8)	26 (17)	19 (12)
Leukopenia	10 (6)	5 (3)	15 (10)	11 (7)
Nonhematologic
Nausea	32 (20)	2 (1)	14 (9)	2 (1)
Pyrexia	44 (28)	5 (3)	30 (20)	0 (0)
Pneumonia	12 (8)	10 (6)	20 (13)	19 (12)
Decreased platelet count	12 (8)	5 (3)	12 (8)	7 (5)
Hypercalcemia	17 (11)	6 (4)	10 (7)	7 (5)
Acute renal failure	15 (10)	12 (8)	6 (4)	5 (3)
Renal failure	10 (6)	8 (5)	3 (2)	2 (1)
Other adverse events of interest
Chest pain	5 (3)	0 (0)	9 (6)	1 (1)
Increased blood creatinine	13 (8)	3 (2)	10 (7)	1 (1)
Decreased creatinine renal clearance	9 (6)	1 (1)	4 (3)	3 (2)
Renal impairment	11 (7)	6 (4)	5 (3)	1 (1)
Dyspnea	23 (15)	2 (1)	13 (9)	0 (0)
Cough	19 (12)	1 (1)	10 (7)	1 (1)
Hypertension	23 (15)	5 (3)	9 (6)	0 (0)
Cardiac failure	7 (5)	3 (2)	1 (1)	1 (1)
Peripheral neuropathy	7 (5)	1 (1)	6 (4)	0 (0)

Adverse events of any grade reported in ⩾20% of patients in either treatment arm and grade ⩾3 adverse events reported in ⩾5% of patients in either treatment arm are listed. Adverse events of clinical interest are also listed.

Grade ⩾3 AEs were similar between groups, with the exception of acute renal failure (carfilzomib, 8% control, 3%) and pneumonia (carfilzomib, 6% control, 12%) (see Table 4). Common grade ⩾3 nonhematologic AEs in the carfilzomib group were renal failure/acute renal failure (13%), disease progression (11%), and pneumonia (6%); common grade ⩾3 nonhematologic AEs in the control group were pneumonia (12%) and disease progression (12%). Hypertension occurred in 15% of carfilzomib patients and 6% of control patients.

Grouped renal failure AEs (that is, azotemia, oliguria, renal failure, acute renal failure and renal impairment) occurred more frequently in patients in the carfilzomib group compared with the control group (24 vs 9%). Overall, these events occurred more frequently in patients with lower CrCl at baseline (36% of all patients with baseline CrCl <30 ml/min, 23% of all patients with baseline CrCl of 30–50 ml/min and 12% of all patients with baseline CrCl ⩾50 ml/min). Furthermore, a majority of the patients who reported grouped acute renal failure AEs had urine protein electrophoresis-measurable disease (carfilzomib group, 74% control group, 71%) compared with 26 and 29%, respectively, of those without urine protein electrophoresis-measureable disease.

Discussion

Patients with advanced RRMM have poor outcomes and few treatment options as they are likely to have been exposed to all major classes of therapeutic agents and are often refractory to at least one agent. The FOCUS study investigated single-agent carfilzomib vs low-dose corticosteroids with optional cyclophosphamide in heavily pretreated patients with RRMM. The study did not meet its primary endpoint; there was no significant improvement in OS for carfilzomib compared with control (HR for OS 0.975; 95% CI 0.760–1.249; P=0.4172). PFS was also similar between the two treatment groups.

The single-agent activity of carfilzomib was demonstrated by the ORR of 19.1%, which was nearly twice as high as that noted with the control group (11.4% one-sided P=0.0305); the proportion of patients achieving a minimal response or better in the carfilzomib group was also higher than that in the control group (31.2 vs 20.8%, respectively).

Incidence rates of AEs were similar between groups, with the exception of an increase in the rate any-grade hypertension (15 vs 6%) and grade ⩾3 grouped renal failure events in the carfilzomib group (24 vs 9%). The increase in renal failure events may be possibly explained by the inclusion of late-line patients with heavy disease burden, with events occurring more frequently in patients with low baseline CrCl. Notably, no increase in peripheral neuropathy was observed with carfilzomib relative to control.

The study design was based on encouraging phase II results observed with single-agent carfilzomib, which showed an ORR of 24% and a median OS of 15.2 months in patients who had received a median of five prior treatment lines (range, 1-20).[10] The efficacy results observed in the present study were comparable to those reported by Siegel et al,[10] while the comparatively shorter median OS of 10.2 months was likely due to differences in enrollment criteria between the two studies. In the phase II PX-171-003-A1 study, patients must have received at least two prior regimens, had platelet counts ⩾50 000/mm3, and CrCl ⩾30 ml/min; the FOCUS study specified that patients had to have received at least three prior regimens, have platelet counts ⩾30 000/mm3, and CrCl ⩾15 ml/min.

Notably, the FOCUS study compared single-agent carfilzomib against an active doublet control regimen. The addition of dexamethasone to carfilzomib should be considered, particularly in patients with suboptimal response, a strategy that had been followed with bortezomib in the APEX study.[21]

Further study limitations may include the open-label nature of the study, which may have led to more patients in the control group initiating their next antimyeloma regimen prior to disease progression or death (that is, censored for PFS due to start of next therapy). It is also worth noting that treatments for RRMM are often most effective in standard-risk patients; enrolling patients with advanced disease, such as those with very poor Eastern Cooperative Oncology Group performance status, renal function and/or low platelet counts, poses a significant hurdle for any single-agent therapy to demonstrate clinical benefit.

In conclusion, the phase III FOCUS study did not meet its primary end point for OS, as the active control arm of corticosteroids and optional cyclophosphamide (in which 95% of patients received cyclophosphamide) performed better than expected and demonstrated activity in this patient population. This active control arm merits further evaluation in patients with RRMM. The safety profile of single-agent carfilzomib was generally consistent with what has been reported previously in heavily pretreated patients with MM, with the exception of increased renal events.[10] Carfilzomib combined with dexamethasone and/or other agents remains an active treatment option for patients with advanced MM,[22] based on positive results from phase III studies in RRMM. The phase III study ASPIRE (NCT01080391) demonstrated that the addition of carfilzomib to lenalidomide-dexamethasone led to a HR for PFS of 0.69 (95% CI, 0.57–0.83) for patients with relapsed MM,[23] and the phase III study ENDEAVOR (NCT01568866) met its primary endpoint of improved PFS with carfilzomib (20/56 mg/m2; 30-min infusion)-dexamethasone vs bortezomib-dexamethasone (HR 0.53; 95% CI 0.44–0.65) in patients with relapsed MM.[24] Therefore, despite the lack of a survival advantage observed in late-line RRMM patients, carfilzomib remains an important component of anti-MM treatment in a variety of settings.