Gaku Harata1,2, Himanshu Kumar3, Fang He4, Kenji Miyazawa5, Kazutoyo Yoda5, Manabu Kawase5, Akira Kubota5, Masaru Hiramatsu5, Samuli Rautava3, Seppo Salminen3. 1. Technical Research Laboratory, Takanashi Milk Products Co., Ltd., Yokohama, 241-0023, Japan. g-harata@takanashi-milk.co.jp. 2. Functional Foods Forum, University of Turku, 20014, Turku, Finland. g-harata@takanashi-milk.co.jp. 3. Functional Foods Forum, University of Turku, 20014, Turku, Finland. 4. Technical Research Laboratory, Takanashi Milk Products Co., Ltd., Yokohama, 241-0023, Japan. ka-hou@takanashi-milk.co.jp. 5. Technical Research Laboratory, Takanashi Milk Products Co., Ltd., Yokohama, 241-0023, Japan.
Abstract
BACKGROUND:Japanese cedar pollinosis (JCP) is a challenging public health problem in Japan. Altered gut microbiota is associated with several diseases, including allergic diseases. However, only a few studies have focused on JCP and the underlying mechanisms for probiotic effects remain unclear. In addition, this study is the first observation of the correlation between the gut microbiota and blood lipid in JCP. METHODS: Faecal samples from JCP subjects were collected before and after treatment with (n = 14) and without (n = 11) LGG-TMC0356-fermented milk for 10 weeks. Gut microbiota composition was characterized from faecal DNA using sequencing of 16S rRNA genes. RESULTS: 16S rRNA-based operational taxonomic unit clustering of the microbiota revealed that LGG-TMC0356-fermented milk significantly altered gut microbiota after 10 weeks of milk consumption, and eight dominant genera of microbes were detected. During the JCP season, the Bacteroidetes/Firmicutes ratio, when compared to baseline, was significantly decreased in subjects at end of the study. Bacteroidetes showed positive correlation with LDL- and HDL-cholesterol levels, whereas Firmicutes showed negative correlation with total cholesterol, LDL- and HDL- cholesterol. CONCLUSIONS: The altered gut microbiota through supplementation of fermented milk containing the study probiotics may be a prospective target for protection against JCP, with beneficial effects on blood lipid levels.
RCT Entities:
BACKGROUND:Japanese cedarpollinosis (JCP) is a challenging public health problem in Japan. Altered gut microbiota is associated with several diseases, including allergic diseases. However, only a few studies have focused on JCP and the underlying mechanisms for probiotic effects remain unclear. In addition, this study is the first observation of the correlation between the gut microbiota and blood lipid in JCP. METHODS: Faecal samples from JCP subjects were collected before and after treatment with (n = 14) and without (n = 11) LGG-TMC0356-fermented milk for 10 weeks. Gut microbiota composition was characterized from faecal DNA using sequencing of 16S rRNA genes. RESULTS: 16S rRNA-based operational taxonomic unit clustering of the microbiota revealed that LGG-TMC0356-fermented milk significantly altered gut microbiota after 10 weeks of milk consumption, and eight dominant genera of microbes were detected. During the JCP season, the Bacteroidetes/Firmicutes ratio, when compared to baseline, was significantly decreased in subjects at end of the study. Bacteroidetes showed positive correlation with LDL- and HDL-cholesterol levels, whereas Firmicutes showed negative correlation with total cholesterol, LDL- and HDL- cholesterol. CONCLUSIONS: The altered gut microbiota through supplementation of fermented milk containing the study probiotics may be a prospective target for protection against JCP, with beneficial effects on blood lipid levels.
Authors: Colin Hill; Francisco Guarner; Gregor Reid; Glenn R Gibson; Daniel J Merenstein; Bruno Pot; Lorenzo Morelli; Roberto Berni Canani; Harry J Flint; Seppo Salminen; Philip C Calder; Mary Ellen Sanders Journal: Nat Rev Gastroenterol Hepatol Date: 2014-06-10 Impact factor: 46.802
Authors: Thomas R Abrahamsson; Hedvig E Jakobsson; Anders F Andersson; Bengt Björkstén; Lars Engstrand; Maria C Jenmalm Journal: J Allergy Clin Immunol Date: 2011-12-06 Impact factor: 10.793
Authors: W Feleszko; J Jaworska; R-D Rha; S Steinhausen; A Avagyan; A Jaudszus; B Ahrens; D A Groneberg; U Wahn; E Hamelmann Journal: Clin Exp Allergy Date: 2007-04 Impact factor: 5.018
Authors: Nadja Larsen; Finn K Vogensen; Frans W J van den Berg; Dennis Sandris Nielsen; Anne Sofie Andreasen; Bente K Pedersen; Waleed Abu Al-Soud; Søren J Sørensen; Lars H Hansen; Mogens Jakobsen Journal: PLoS One Date: 2010-02-05 Impact factor: 3.240
Authors: Nanda Burger-van Paassen; Audrey Vincent; Patrycja J Puiman; Maria van der Sluis; Janneke Bouma; Günther Boehm; Johannes B van Goudoever; Isabelle van Seuningen; Ingrid B Renes Journal: Biochem J Date: 2009-05-13 Impact factor: 3.857
Authors: Ruth E Ley; Fredrik Bäckhed; Peter Turnbaugh; Catherine A Lozupone; Robin D Knight; Jeffrey I Gordon Journal: Proc Natl Acad Sci U S A Date: 2005-07-20 Impact factor: 11.205