Literature DB >> 27408489

A practical way to prepare primer human chondrocyte culture.

Mehmet Isyar1, Ibrahim Yilmaz2, Duygu Yasar Sirin3, Sercan Yalcin1, Olcay Guler1, Mahir Mahirogullari1.   

Abstract

Biological cartilage repair is one of the most important targets for orthopedic surgeons currently. For this purpose, it is mandatory to know how to prepare a chondrocyte culture. In this study, our purpose was to introduce a method enabling orthopedic surgeons to practice their knowledge and skills on molecular experimental setup at cellular level, based on our experiences from previous pilot studies. Thus, we believe it will encourage orthopedic surgeons.

Entities:  

Keywords:  Chondrocyte; Osteochondral tissue; Primary cell culture

Year:  2016        PMID： 27408489      PMCID： PMC4919283          DOI： 10.1016/j.jor.2016.03.008

Source DB:  PubMed          Journal:  J Orthop        ISSN： 0972-978X


  27 in total

1.  Insulin-transferrin-selenium prevent human chondrocyte dedifferentiation and promote the formation of high quality tissue engineered human hyaline cartilage.

Authors:  K H Chua; B S Aminuddin; N H Fuzina; B H I Ruszymah
Journal:  Eur Cell Mater       Date:  2005-06-17       Impact factor: 3.942

2.  Dynamic three-dimensional culture methods enhance mesenchymal stem cell properties and increase therapeutic potential.

Authors:  Jessica E Frith; Brian Thomson; Paul G Genever
Journal:  Tissue Eng Part C Methods       Date:  2010-08       Impact factor: 3.056

3.  Tissue engineering and regenerative medicine: from first principles to state of the art.

Authors:  Joseph Vacanti
Journal:  J Pediatr Surg       Date:  2010-02       Impact factor: 2.545

Review 4.  Methods to study cartilage and bone development.

Authors:  Anenisia C Andrade; Dionisios Chrysis; Laura Audi; Ola Nilsson
Journal:  Endocr Dev       Date:  2011-08-22

5.  Evaluation of novel in situ synthesized nano-hydroxyapatite/collagen/alginate hydrogels for osteochondral tissue engineering.

Authors:  Li Zheng; Xianfang Jiang; Xuening Chen; Hongsong Fan; Xingdong Zhang
Journal:  Biomed Mater       Date:  2014-10-30       Impact factor: 3.715

Review 6.  Regulation of chondrogenesis by protein kinase C: Emerging new roles in calcium signalling.

Authors:  Csaba Matta; Ali Mobasheri
Journal:  Cell Signal       Date:  2014-01-17       Impact factor: 4.315

7.  Stable propagation of human embryonic and induced pluripotent stem cells on decellularized human substrates.

Authors:  Sheena Abraham; Steven D Sheridan; Bradley Miller; Raj R Rao
Journal:  Biotechnol Prog       Date:  2010 Jul-Aug

8.  Isolation and characterization of human intestinal mast cells.

Authors:  Axel Lorentz; Gernot Sellge; Stephan C Bischoff
Journal:  Methods Mol Biol       Date:  2015

9.  Osteoarthritic chondrocyte-secreted morphogens induce chondrogenic differentiation of human mesenchymal stem cells.

Authors:  Aereas Aung; Gunjan Gupta; Ghassemian Majid; Shyni Varghese
Journal:  Arthritis Rheum       Date:  2011-01

Review 10.  Toward stem cell systems biology: from molecules to networks and landscapes.

Authors:  B D MacArthur; A Ma'ayan; I R Lemischka
Journal:  Cold Spring Harb Symp Quant Biol       Date:  2009-03-27
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  11 in total

1.  Is there a treatment protocol in which platelet-rich plasma is effective?

Authors:  Ibrahim Yilmaz; Semih Akkaya; Mehmet Isyar; Ahmet Güray Batmaz; Olcay Guler; Kadir Oznam; Akin Ugras; Mahir Mahiroğullari
Journal:  J Orthop       Date:  2016-07-02

2.  Iopromide- and gadopentetic acid-derived preparates used in MR arthrography may be harmful to chondrocytes.

Authors:  Kadir Oznam; Duygu Yasar Sirin; Ibrahim Yilmaz; Yasin Emre Kaya; Mehmet Isyar; Seyit Ali Gumustas; Hanefi Ozbek; Semih Akkaya; Arda Kayhan; Mahir Mahirogullari
Journal:  J Orthop Surg Res       Date:  2017-06-26       Impact factor: 2.359

3.  Can a Biodegradable Implanted Bilayered Drug Delivery System Loaded with BMP-2/BMP-12 Take an Effective Role in the Biological Repair Process of Bone-Tendon Injuries? A Preliminary Report.

Authors:  Baran Komur; Yener Akyuva; Numan Karaslan; Mehmet Isyar; Seyit Ali Gumustas; Ibrahim Yilmaz; Semih Akkaya; Duygu Yasar Sirin; Cagri Ata Mutlu; Ahmet Guray Batmaz; Olcay Guler; Mahir Mahirogullari
Journal:  J Pharm (Cairo)       Date:  2017-06-04

4.  The association between different molecular weights of hyaluronic acid and CHAD, HIF-1α, COL2A1 expression in chondrocyte cultures.

Authors:  Duygu Yasar Sirin; Necati Kaplan; Ibrahim Yilmaz; Numan Karaarslan; Hanefi Ozbek; Yener Akyuva; Yasin Emre Kaya; Kadir Oznam; Nuray Akkaya; Olcay Guler; Semih Akkaya; Mahir Mahirogullari
Journal:  Exp Ther Med       Date:  2018-03-09       Impact factor: 2.447

5.  Alterations in characteristics of canine articular chondrocytes in non-passaged long-term monolayer culture: Matter of differentiation, dedifferentiation and redifferentiation.

Authors:  Ekkapol Akaraphutiporn; Takafumi Sunaga; Eugene C Bwalya; Ryosuke Echigo; Masahiro Okumura
Journal:  J Vet Med Sci       Date:  2020-04-30       Impact factor: 1.267

6.  Pregabalin treatment for neuropathic pain may damage intervertebral disc tissue.

Authors:  Numan Karaarslan; Ibrahim Yilmaz; Duygu Yasar Sirin; Hanefi Ozbek; Necati Kaplan; Yasin Emre Kaya; Yener Akyuva; Mehmet Sabri Gurbuz; Kadir Oznam; Ozkan Ates
Journal:  Exp Ther Med       Date:  2018-06-12       Impact factor: 2.447

7.  Evaluation of the effects of pregabalin on chondrocyte proliferation and CHAD, HIF-1α, and COL2A1 gene expression.

Authors:  Duygu Yasar Sirin; Numan Karaarslan
Journal:  Arch Med Sci       Date:  2018-02-02       Impact factor: 3.318

8.  Effect of naproxen on proliferation and differentiation of primary cell cultures isolated from human cartilage tissue.

Authors:  Numan Karaarslan; Ahmet Guray Batmaz; Ibrahim Yilmaz; Hanefi Ozbek; Tezcan Caliskan; Duygu Yasar Sirin; Necati Kaplan; Kadir Oznam; Ozkan Ates
Journal:  Exp Ther Med       Date:  2018-06-26       Impact factor: 2.447

9.  Does Nimodipine, a Selective Calcium Channel Blocker, Impair Chondrocyte Proliferation or Damage Extracellular Matrix Structures?

Authors:  Necati Kaplan; Ibrahim Yilmaz; Numan Karaarslan; Yasin E Kaya; Duygu Y Sirin; Hanefi Ozbek
Journal:  Curr Pharm Biotechnol       Date:  2019       Impact factor: 2.837

Review 10.  A comprehensive analysis to understand the mechanism of action of balneotherapy: why, how, and where they can be used? Evidence from in vitro studies performed on human and animal samples.

Authors:  Sara Cheleschi; Ines Gallo; Sara Tenti
Journal:  Int J Biometeorol       Date:  2020-03-21       Impact factor: 3.787
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