Association between pretreatment Glasgow prognostic score and gastric cancer survival and clinicopathological features: a meta-analysis.

BACKGROUND: Glasgow prognostic score (GPS) is widely known as a systemic inflammatory-based marker. The relationship between pretreatment GPS and gastric cancer (GC) survival and clinicopathological features remains controversial. The aim of the study was to conduct a meta-analysis of published studies to evaluate the association between pretreatment GPS and survival and clinicopathological features in GC patients.
METHODS: We searched PubMed, Embase, MEDLINE, and BioMed databases for relevant studies. Combined analyses were used to assess the association between pretreatment GPS and overall survival, disease-free survival, and clinicopathological parameters by Stata Version 12.0.
RESULTS: A total of 14 studies were included in this meta-analysis, including 5,579 GC patients. The results indicated that pretreatment high GPS (HGPS) predicted poor overall survival (hazard ratio =1.51, 95% CI: 1.37-1.66, P<0.01) and disease-free survival (hazard ratio =1.45, 95% CI: 1.26-1.68, P<0.01) in GC patients. Pretreatment HGPS was also significantly associated with advanced tumor-node-metastasis stage (odds ratio [OR] =3.09, 95% CI: 2.11-4.53, P<0.01), lymph node metastasis (OR =4.60, 95% CI: 3.23-6.56, P<0.01), lymphatic invasion (OR =3.04, 95% CI: 2.00-4.62, P<0.01), and venous invasion (OR =3.56, 95% CI: 1.81-6.99, P<0.01).
CONCLUSION: Our meta-analysis indicated that pretreatment HGPS could be a predicative factor of poor survival outcome and clinicopathological features for GC patients.

Introduction

Gastric cancer (GC) is one of the most common cancers in the world.1 In recent years, although surgery, chemoradiotherapy, and targeted therapy make great progress to prolong survival of GC patients, GC is still the third leading cause of cancer-related death worldwide mainly because of tumor local recurrence and distant metastasis.1,2 Therefore, it is essential to identify GC patients with a high-risk of tumor recurrence and poor prognosis. For this purpose, both clinicians and researchers have made great efforts to seek practical biomarkers that can predict progression and survival in GC patients.

In the current clinical work, pathological tumor–node–metastasis (TNM) stage is a common predictive factor of predicting the prognosis and making treatment plans for GC patients, but heterogeneity of prognosis still exists in the same stage.3 This leaves a large space to search supplementary biomarkers for better predicting the prognosis of GC patients. It is increasingly recognized that variations within clinical outcomes in cancer patients were influenced by not only clinicopathological characteristics but also the host systemic inflammatory response.4 The connection between systemic inflammatory response and tumors has been researched in both animal models and clinical trials in the past years.5,6

Glasgow prognostic score (GPS) as an inflammation-based prognostic score is composed of serum C-reactive protein (CRP) and albumin level. The first study on GPS reported by Forrest et al7 in 2003 showed that it could be an indicator of prognosis for non-small-cell lung cancer patients. Subsequently, increasing evidence revealed that GPS acquired its prognostic significance in patients with esophageal cancer,8 pancreatic cancer,9 colorectal cancer,10 and so on. Meanwhile, increased level of GPS could predict poor survival in GC patients.11 However, due to variance in the study design, some authors did not agree with the former results.12 The impact of GPS on GC patients’ prognosis still remains controversial. Thus, we searched the available studies and conducted this meta-analysis in order to evaluate the prognostic role of GPS in GC. The relationship between GPS and clinicopathological parameters was also investigated.

Materials and methods

Literature search

We searched PubMed, Embase, MEDLINE, and BioMed databases for studies that assessed the prognostic role of GPS for GC patients. The keywords used for research were “Glasgow prognostic score” or “GPS”, “survival” or “outcome”, “clinicopathological feature”, and “gastric cancer” or “stomach carcinoma”. The last search was updated in December 31, 2015. Additionally, potentially related articles were also retrieved by the references in the identified articles.

Study inclusion and exclusion criteria

Inclusion criteria for selecting the articles in our analysis were as follows: 1) studies in which the patients were diagnosed as GC based on pathological examination; 2) studies that investigated the association between pretreatment GPS and clinicopathological characteristics, overall survival (OS), and disease-free survival (DFS); 3) studies that were not directly reporting hazard ratio (HR) with its 95% confidence interval (CI) were allowed if we could reconstruct HR with its 95% CI by data reported;13 and 4) papers that were published in English. Exclusion criteria were as follows: 1) reviews, case reports, or laboratory studies; 2) studies without available full text or sufficient data for reconstructing HR with its 95% CI; and 3) studies containing duplicate data.

Data extraction

For each study, the following items were extracted: 1) first author, year of publication, country of the studied population, sample size, age, predominant treatment, and follow-up period; 2) clinicopathological parameters including TNM stage, lymph node metastasis, lymphatic invasion, venous invasion, differentiated degree, and carcinoembryonic antigen level; 3) survival data including OS and DFS; and 4) the cut-off value of GPS.

Quality assessment

Two reviewers (Chun-Xiao Zhang and Shu-Yi Wang) independently implemented quality assessment in the included studies by using the Newcastle–Ottawa Quality Assessment Scale (NOS).14 The NOS comprised three parameters of quality: selection (0–4 points), comparability (0–2 points), and outcome assessment (0–3 points). The maximum score is 9 points, and NOS scores of ≥6 were assigned as high-quality studies. Any disagreement was resolved by discussion.

Statistical analysis

HR with its 95% CI was obtained directly from each literature or estimated by data reported according to the methods illustrated by Parmar et al.13 For analyzing the correlation between GPS and clinicopathological parameters, odds ratio (OR) with its 95% CI was combined as the effective value. If both univariate and multivariate analyses were reported in the same study, multivariate analysis was chosen for the meta-analysis. Heterogeneity among pooled results was assessed using Cochran’s Q test and Higgins I2 statistic. P<0.10 and I2>50% were considered the values that indicated significant heterogeneity, and the random-effects model was applied. If not, the fixed-effects model was performed. The potential publication bias was evaluated by Begg’s funnel plot and Egger’s linear regression test. The influence of publication bias on the overall effect was assessed by the “trim and fill” method described by Duval and Tweedie.15 All statistical analyses were two-sided, and the significant level was defined as 5%. Statistical analyses were performed by Stata Version 12.0 (StataCorp LP, College Station, TX, USA).

Results

Description of the included studies

According to the aforementioned search terms, 132 studies were retrieved. By intensively reading the potential articles, 118 studies were excluded. Lastly, 14 studies published between 2011 and 2015 were included in this meta-analysis (Figure 1).11,12,16–27 Characteristics of included studies are summarized in Table 1. Fourteen retrospective studies were included containing 5,579 GC patients. Seven studies were from Japan, four from the People’s Republic of China, two from Korea, and one from Italy. The sample size of included articles ranged from 102 to 1,710 GC patients. The cut-off values of GPS were diverse, and GPS of 0 or 1 point was assigned for each CRP level less than or greater than the cut-off value (3 mg/L, 5 mg/L, or 10 mg/L) and albumin level greater than or less than the cut-off value (35 g/L or 38 g/L), aggregating score ranging from 0 to 2. GPS of 2 was defined as high GPS (HGPS), and GPS of 0 or 1 was defined as low GPS (LGPS). HR with its 95% CI was directly extracted from the original literature in 13 studies and estimated by the data reported in the remaining one study.

Figure 1

The flow chart of study selection.

Abbreviations: DFS, disease-free survival; OS, overall survival.

Table 1

Characteristics of included studies

Study	Country	Sample size	Predominant treatment	TNM	Follow-up (months)a	Cut-off value	No (GPS 0/1/2)	Survival analysis	NOS score
Aurello et al16	Italy	102	Surgery	I–IV	40.8 (8–107)	CRP =10 mg/L; Alb =35 g/L	49/25/28	OS, DFS	6
Hirashima et al17	Japan	294	Surgery	I–IV	31.5 (1–91)	CRP =5 mg/L; Alb =38 g/L	174/84/36	OS	8
Hwang et al18	Korea	402	Chemotherapy	IV	11.4 (1.1–58.5)	CRP =10 mg/L; Alb =35 g/L	238/111/53	OS	6
Ishizuka et al19	Japan	425	Surgery	I–IV	49.6 (0.3–147.6)	CRP =10 mg/L; Alb =35 g/L	298/99/28	OS	8
Jeong et al20	Korea	104	Chemotherapy	IV	11.9	CRP =10 mg/L; Alb =35 g/L	58/29/17	OS	6
Jiang et al11	Japan	1,710	Surgery	I–IV	43 (1–123)	CRP =10 mg/L; Alb =35 g/L	1,565/78/67	OS	8
Li et al21	People’s Republic of China	384	Chemotherapy	III–IV	13.9	CRP =10 mg/L; Alb =35 g/L	145/66/5	OS	6
Liu et al22	People’s Republic of China	455	Surgery	I–III	25 (1–76)	CRP =10 mg/L; Alb =35 g/L	350/92/13	OS	7
Mohri et al23	Japan	197	Surgery	I–III	51 (23–82)	CRP =5 mg/L; Alb =35 g/L	114/53/30	DFS	7
Nozoe et al24	Japan	232	Surgery	I–IV	1.67–111	CRP =10 mg/L; Alb =35 g/L	140/64/28	OS	8
Pan et al25	People’s Republic of China	207	Surgery	I–IV	37 (3–83)	CRP =10 mg/L; Alb =35 g/L	103/71/33	OS, DFS	6
Takeno et al12	Japan	552	Surgery	I–IV	NR	CRP =3 mg/L; Alb =35 g/L	411/75/66	OS	8
Tanaka et al26	Japan	191	Surgery	IV	13.3	CRP =10 mg/L; Alb =35 g/L	132/23/36	OS	7
Wang et al27	People’s Republic of China	324	Surgery	III	39.9 (23.8–57.4)	CRP =10 mg/L; Alb =35 g/L	248/58/18	OS, DFS	7

Note:

Median/range.

Abbreviations: GPS, Glasgow prognostic score; NOS, Newcastle–Ottawa Quality Assessment Scale; CRP, C-reactive protein; Alb, albumin; OS, overall survival; DFS, disease-free survival; NR, not reported.

GPS and survival outcome in GC

There were 13 studies reporting the relationship between pretreatment GPS and OS in GC patients.11,12,16–22,24–27 With no obvious heterogeneity (I2=31.9%, P=0.13), the pooled HR of 1.51 (95% CI: 1.37–1.66, P<0.01) implied that GC patients with HGPS were expected to have poor OS (Figure 2). Then, we conducted subgroup analysis according to confounders such as predominant treatment, country, sample size, and NOS score.

Figure 2

The forest plot between pretreatment GPS and OS in GC patients.

Abbreviations: GPS, Glasgow prognostic score; OS, overall survival; GC, gastric cancer; HR, hazard ratio; CI, confidence interval; HGPS, high Glasgow prognostic score; LGPS, low Glasgow prognostic score.

Stratification by predominant treatment, we found that the pooled HRs were 1.41 (95% CI: 1.26–1.58, P<0.01) for patients treated by surgery and 1.78 (95% CI: 1.48–2.13, P<0.01) for patients treated by chemotherapy. Subgroup analyses by country indicated that HGPS predicted poor prognosis for patients both in Japan (HR =1.57, 95% CI: 1.28–1.93, P<0.01) and in other countries (HR =1.49, 95% CI: 1.33–1.66, P <0.01). In addition, subgroup analysis showed that HGPS could also predict poor prognosis for GC regardless of sample size (<300 vs ≥300) and NOS score (<7 vs ≥7) (Table 2).

Table 2

Results of subgroup analysis

Subgroup	No of studies	Random-effects model		Fixed-effects model		Heterogeneity
		HR (95% CI)	P-value	HR (95% CI)	P-value	I2 (%)	P-value
Predominant treatment
Surgery	10	1.46 (1.26–1.68)	<0.01	1.41 (1.26–1.58)	<0.01	27.3	0.19
Chemotherapy	3	1.78 (1.48–2.13)	<0.01	1.78 (1.48–2.13)	<0.01	0.0	0.70
Country
Japan	6	1.66 (1.29–2.14)	<0.01	1.57 (1.28–1.93)	<0.01	23.4	0.26
Other	7	1.51 (1.30–1.77)	<0.01	1.49 (1.33–1.66)	<0.01	44.5	0.09
Sample size
<300	6	1.73 (1.35–2.20)	<0.01	1.60 (1.34–1.92)	<0.01	28.7	0.22
≥300	7	1.48 (1.27–1.73)	<0.01	1.47 (1.31–1.65)	<0.01	39.5	0.13
NOS score
<7	5	1.65 (1.41–1.92)	<0.01	1.64 (1.42–1.88)	<0.01	16.2	0.31
≥7	8	1.46 (1.22–1.74)	<0.01	1.39 (1.22–1.59)	<0.01	30.6	0.18

Abbreviations: HR, hazard ratio; CI, confidence interval; NOS, Newcastle–Ottawa Quality Assessment Scale.

There were four studies reporting the relationship between pretreatment GPS and DFS in GC patients.16,23,25,27 The pooled result (HR =1.45, 95% CI: 1.26–1.68, P<0.01) showed significant correlation between HGPS and short DFS with no heterogeneity (I2=0%, P=0.46; Figure 3).

Figure 3

The forest plot between pretreatment GPS and DFS in GC patients.

Abbreviations: GPS, Glasgow prognostic score; DFS, disease-free survival; GC, gastric cancer; HR, hazard ratio; CI, confidence interval; HGPS, high Glasgow prognostic score; LGPS, low Glasgow prognostic score.

GPS and clinicopathological features in GC

Six studies presented the relationship between pretreatment GPS and TNM stage in GC patients.11,12,17,19,23,24 The combined OR of 3.09 (95% CI: 2.11–4.53, P<0.01) displayed that HGPS was associated with advanced TNM stage for GC patients, with no significant heterogeneity (I2=46.9%, P=0.09; Figure 4A).

Figure 4

The forest plot between pretreatment GPS and clinicopathological parameters in GC.

Notes: (A) TNM stage (III vs I and II); (B) lymph node metastasis (positive vs negative); (C) lymphatic invasion (positive vs negative); and (D) venous invasion (positive vs negative). Weights are random-effects analysis.

Abbreviations: GPS, Glasgow prognostic score; GC, gastric cancer; TNM, tumor–node–metastasis; OR, odds ratio; CI, confidence interval; HGPS, high Glasgow prognostic score; LGPS, low Glasgow prognostic score.

Five studies presented the relationship between pretreatment GPS and lymph node metastasis in GC patients.12,17,19,23,24 The combined OR of 4.60 (95% CI: 3.23–6.56, P<0.01) displayed that GC patients with HGPS were prone to lymph node metastasis with no obvious heterogeneity (I2=45.4%, P=0.12; Figure 4B).

There were five studies reporting the relationship between pretreatment GPS and lymphatic invasion in GC patients.12,17,19,23,24 The pooled estimate (OR =3.04, 95% CI: 2.00–4.62, P<0.01) showed that HGPS was markedly associated with lymphatic invasion with minor heterogeneity (I2=36.5%, P=0.18; Figure 4C).

Five studies showed the connection between pretreatment GPS and venous invasion in GC patients.12,17,19,23,24 Although with heterogeneity (I2=71.3%, P<0.01), the combined result (OR =3.56, 95% CI: 1.81–6.99, P<0.01) revealed an obvious relevance between HGPS and venous invasion (Figure 4D).

No statistically significant difference was found in the relationship between pretreatment GPS and differentiated degree (poor vs well or moderate) and carcinoembryonic antigen level (≥5 ng mL−1 vs <5 ng mL−1) (Table 3).

Table 3

Relationship between pretreatment GPS and differentiated degree and CEA level

Factors	OR	95% CI	P-value
Differentiated degreea	1.02	0.89–1.17	0.79
CEA levelb	2.13	0.51–8.87	0.30

Notes:

Poor vs well or moderate,

≥5 ng mL−1 vs <5 ng mL−1.

Abbreviations: GPS, Glasgow prognostic score; CEA, carcinoembryonic antigen; OR, odds ratio; CI, confidence interval.

Publication bias

We evaluated publication bias using Begg’s funnel plot and Egger’s linear regression test. Publication bias was found only in OS (P=0.04 for Begg’s test and P=0.02 for Egger’s test; Figure 5A). Therefore, the “trim and fill” analysis was further performed. As a result, there were four studies hypothetically remained unpublished, and recalculated result did not change significantly (HR =1.46, 95% CI: 1.33–1.60, P<0.01), indicating the stability of the result (Figure 5B).

Figure 5

Funnel plot of included studies reporting OS in GC patients.

Notes: (A) Begg’s funnel plot for the assessment of potential publication bias; (B) funnel plot adjusted with trim and fill method. Circles: included studies. Squares: presumed missing studies.

Abbreviations: OS, overall survival; GC, gastric cancer; HR, hazard ratio; SE, standard error.

Discussion

Systemic inflammatory response played a crucial role in the development and progression of various tumors.28 Several biomarkers based on inflammation including GPS,29 neutrophil–lymphocyte ratio,30 and platelet–lymphocyte ratio31 had been reported to be independent prognostic predictors for cancer patients. However, it was not clear which of these biomarkers better predicted survival for GC patients.

Many recent studies indicated that pretreatment GPS was superior to other biomarkers in predicting survival in either operable GC patients16,27 or inoperable advanced GC patients.20,21 Besides, GPS, which is easily obtained by peripheral blood test before treatment, can give a preoperative risk assessment, whereas TNM stage and lymphovascular invasion give a postoperative risk assessment. Preoperative HGPS would identify patients at high risk of tumor recurrence and poor prognosis, helping clinicians to focus on these patients who would need more careful TNM stage, rigorous follow-up, and imperative adjuvant treatment. Pan et al25 showed that adjuvant chemotherapy after resection could significantly prolong OS and DFS in GC patients with LGPS, but not with HGPS. The result was similar to the study of Wang et al27 who reported that in stage III GC patients receiving adjuvant chemotherapy, there was a significant decrease in the median survival for those patients with HGPS comparing with those patients with LGPS, which suggested that patients with HGPS derived little survival benefit from active treatment. Therefore, GPS could not only simply represent the host systemic inflammatory response but also importantly be used to predict survival outcome and to individualize treatment plans for GC patients.

The results of our study revealed that pretreatment HGPS could predict poor OS and DFS in GC patients. Meanwhile, there was an obvious relationship between pretreatment HGPS and clinicopathological parameters, such as TNM stage, lymph node metastasis, lymphatic invasion, and venous invasion. According to the above-mentioned results, GPS is a promising prognostic biomarker of GC patients assisting in predicting prognosis and identifying treatment plans.

There were some limitations to this meta-analysis. First, language selection may bring bias. We have restricted our analysis to published studies written in English, other languages such as Japanese and Korean were excluded based on language criteria. This may result in language bias leading to an overestimation of effect sizes.32 Second, heterogeneity among included studies was observed in the results. This may be because of confounding factors, such as different characteristics of enrolled patients, sample size, treatment method, follow-up period, and cut-off value of GPS. We handled the issue of heterogeneity by a rigorous methodological approach that utilized random-effects model for more conservative estimate. Moreover, the funnel plot analysis of OS showed some asymmetry, and publication bias was confirmed by Begg’s test and Egger’s test. Thus, the pooled result may be somehow overvalued. But the followed “trim and fill” analysis did not change the general result, which further reinforced the prognostic role of GPS in GC patients.

Conclusion

Our meta-analysis indicated that pretreatment HGPS, namely elevated CRP and reduced albumin level, was associated with poor survival and clinicopathological features in GC. It might be used as a prognostic biomarker for GC and figure out the high-risk patients who may not benefit from the anti-tumor therapies and consequently adjust treatment strategies. However, large-scale prospective investigations need to be conducted to better understand the prognostic role of GPS in GC patients.