Structural basis for acyl-group discrimination by human Gcn5L2.

Gcn5 is a conserved acetyltransferase that regulates transcription by acetylating the N-terminal tails of histones. Motivated by recent studies identifying a chemically diverse array of lysine acyl modifications in vivo, the acyl-chain specificity of the acetyltransferase human Gcn5 (Gcn5L2) was examined. Whereas Gcn5L2 robustly catalyzes lysine acetylation, the acyltransferase activity of Gcn5L2 becomes progressively weaker with increasing acyl-chain length. To understand how Gcn5 discriminates between different acyl-CoA molecules, structures of the catalytic domain of human Gcn5L2 bound to propionyl-CoA and butyryl-CoA were determined. Although the active site of Gcn5L2 can accommodate propionyl-CoA and butyryl-CoA without major structural rearrangements, butyryl-CoA adopts a conformation incompatible with catalysis that obstructs the path of the incoming lysine residue and acts as a competitive inhibitor of Gcn5L2 versus acetyl-CoA. These structures demonstrate how Gcn5L2 discriminates between acyl-chain donors and explain why Gcn5L2 has weak activity for acyl moieties that are larger than an acetyl group.

Introduction

Lysine acetylation is an abundant post-translational modification (Weinert et al., 2011 ▸; Choudhary et al., 2009 ▸) that changes the overall size and charge of the modified residue. Several classes of enzymes are known to catalyze site-specific lysine acetylation (Roth et al., 2001 ▸; Yang, 2004 ▸; Marmorstein & Trievel, 2009 ▸), many of which localize to the nucleus and modify lysine residues on histones (Lee & Workman, 2007 ▸). These enzymes are collectively referred to as either histone acetyltransferases (HATs) or lysine (K) acetyltransferases (KATs), the latter to reflect their ability to acetylate non­histone substrates (Glozak et al., 2005 ▸). KATs are divided into several main families based on both structural similarity and the presence of sequence conservation within their catalytic domains (Marmorstein & Trievel, 2009 ▸). Although different KAT families employ distinct kinetic mechanisms to catalyze acetyl transfer, they all share a common dependence on the nucleotide cofactor acetyl-CoA as an acetyl donor (Berndsen & Denu, 2008 ▸).

Gcn5 is a member of the GNAT (Gcn5-related -acetyltransferase) family of histone acetyltransferases that acetylate the N-terminal tails of histones H3 and H2B at the promoters of inducible genes (Grant et al., 1997 ▸) and are broadly implicated in transcriptional regulation (Huisinga & Pugh, 2004 ▸). The kinetic mechanism of Gcn5 has been studied extensively (Tanner et al., 1999 ▸; Tanner, Langer, Kim et al., 2000 ▸) and the Gcn5 catalytic domain from several organisms has been crystallized in the presence of various combinations of substrates (Roth et al., 2001 ▸; Poux et al., 2002 ▸). The active site of Gcn5 contains two grooves where acetyl-CoA and peptide bind, which intersect near the β-mercaptoethylamine moiety of coenzyme A and the target lysine (Rojas et al., 1999 ▸; Poux et al., 2002 ▸). The ternary complex between Gcn5, acetyl-CoA and peptide forms through a fully ordered mechanism (Tanner, Langer, Kim et al., 2000 ▸), as binding to acetyl-CoA brings about a structural rearrangement that widens the peptide-binding groove within the Gcn5 active site (Clements et al., 1999 ▸; Trievel et al., 1999 ▸; Rojas et al., 1999 ▸; Lin et al., 1999 ▸). Because Gcn5 primarily recognizes features of the CoA pantetheine arm and not the acetyl group (Poux et al., 2002 ▸; Clements et al., 1999 ▸; Rojas et al., 1999 ▸; Lin et al., 1999 ▸), Gcn5 binds with similar affinity to acetyl-CoA and free CoA (Tanner, Langer, Kim et al., 2000 ▸). Whether the active site of Gcn5 can accommodate other kinds of CoA molecules with bulkier acyl chains, however, is not known.

Recent studies have found that lysine residues are modified by a chemically diverse array of acyl chains in vivo (Lee, 2013 ▸; Lin et al., 2012 ▸), raising the possibility that some KATs might be able to utilize acyl-CoA cofactors other than acetyl-CoA to catalyze lysine acylation. Members of three different KAT families catalyze lysine acylation in vitro: p300/CBP catalyzes the propionylation, butyrylation and crotonylation of histones and p53 (Chen et al., 2007 ▸; Sabari et al., 2015 ▸), yeast Esa1, a member of the MYST family of acetyltransferases, catalyzes the propionylation of histone H4 peptides (Berndsen et al., 2007 ▸), and human P/CAF, which is closely related by sequence homology to Gcn5, catalyzes the propionylation of histone H3 peptides (Leemhuis et al., 2008 ▸). Some KATs are clearly promiscuous with regards to acyl-chain identity, but the mechanisms employed by acyltransferases to discriminate between different acyl-CoA molecules are still largely unknown.

In this study, we characterize the acyl-chain specificity of human Gcn5 (Gcn5L2), which catalyzes the acetylation of histone peptides much more quickly than either propionylation or butyrylation. To understand how Gcn5 discriminates between the different acyl-chain donors, we determined structures of the catalytic domain of human Gcn5L2 in complex with propionyl-CoA and butyryl-CoA to 2.0 and 2.1 Å resolution, respectively. These structures reveal that the active site of Gcn5 can accommodate longer acyl chains without major structural rearrangements, but that the butyryl chain would sterically clash with an incoming lysine residue. Consistent with this active-site architecture, we show that butyryl-CoA acts as a competitive inhibitor versus acetyl-CoA for human Gcn5L2. These findings raise the possibility that some acyl-CoA molecules might function as natural inhibitors of Gcn5 in vivo, and have important implications for the regulation of KATs in response to metabolic changes.

Methods

Protein expression and purification

A plasmid encoding the His-tagged catalytic domain of human Gcn5L2 (hsGcn5L2) under T7 induction was obtained from Addgene (Plasmid No. 25482). The protein was expressed and purified as described previously (Schuetz et al., 2007 ▸). Purified protein was dialyzed into 20 mM HEPES pH 7.5, 150 mM NaCl, 1 mM DTT, concentrated to 9 mg ml−1, flash-frozen in liquid nitrogen and stored at −80°C.

Enzymatic assays

Kinetic measurements comparing rates of acetylation, propionylation and butyrylation were performed using the 5,5′-dithiobis-(2-nitrobenzoic acid) (DTNB) assay (Berndsen et al., 2007 ▸) with the following modifications. Reactions contained 10 µM hsGcn5L2 catalytic domain, 250 µM histone H3 peptide amino acids 1–21 (purchased from United Peptide at >90% purity), 100 mM HEPES pH 7.6, 50 mM NaCl and 500 µM acetyl-CoA (Sigma catalog No. A2181), propionyl-CoA (Sigma catalog No. P5397) or butyryl-CoA (Sigma catalog No. B1508). Each reaction was incubated for 5 min at 37°C before adding acyl-CoA, and then maintained at 37°C for the remainder of the experiment. Initially, six data points were collected to find a time frame over which acyl-CoA consumption was linear with time. The reaction was quenched at the indicated time points by the addition of two volumes of quenching buffer (3.2 M guanidine–HCl, 100 mM sodium phosphate pH 6.8). After all the samples had been collected, one volume of 4 mM DTNB (Sigma catalog No. D218200) dissolved in 100 mM sodium phosphate pH 6.8 was added. Samples were moved to a 384-well polystyrene clear-bottom plate (Grenier Bio-One) and the absorbance at 412 nM was measured in a POLARstar Omega plate reader (BMG Labtech). Absorbances were converted to concentrations using a standard curve generated by reacting increasing concentrations of CoA (Sigma catalog No. C3019) with DTNB using an extinction coefficient for 3-thio-6-nitrobenzoate (TNB) of ∊412 nm = 13 700 M −1 cm−1. Subsequent reactions were performed in triplicate and quenched after 0.5 min (acetyl-CoA), 5 min (propionyl-CoA) or 20 min (butyryl-CoA). All acylation rates were corrected by subtracting the rate of acyl-CoA consumption by Gcn5L2 in the absence of peptide.

Steady-state kinetic titrations varying the acetyl-CoA or butyryl-CoA concentration were performed with a continuous spectrophotometric assay as described previously (Berndsen & Denu, 2005 ▸). Briefly, the acetyl-CoA or butyryl-CoA concentration was varied between 0.25 and 100 µM in the presence of 50 nM hsGcn5L2 and 300 µM histone H3 peptide. Reactions were performed in a total volume of 50 µl at 37°C in 384-well plates (Greiner Bio-One) and were initiated with the acyl-CoA. The absorbance at 340 nm was monitored continuously using a POLARstar Omega plate reader (BMG Labtech) for 5–20 min and converted into the molar concentration of NADH using Beer’s law, assuming ∊340 nm = 6220 M −1 cm−1. As controls, rate measurements were performed at each concentration of acyl-CoA in the absence of peptide. Each measurement was performed in triplicate, and reaction velocities in the presence of peptide were blanked by the rate of reaction in the absence of peptide. Blanked rates were normalized to enzyme concentration, plotted as a function of substrate concentration and fitted to the Michaelis–Menten equation using nonlinear least-squares regression in GraphPad Prism 5. Butyryl-CoA inhibition measurements were also performed with the enzyme-coupled assay. Reaction velocities were measured in the presence of 0.5–10 µM acetyl-CoA and 50 nM hsGcn5L2 with increasing concentrations of butyryl-CoA (0, 50, 100 or 300 µM). Under these conditions, consumption of butyryl-CoA by hsGcn5L2 is undetectable by the same assay. Blanked rates were normalized to enzyme concentration and the resulting curves were globally fitted to a competitive-inhibition model in GraphPad Prism 5.

HAT-domain crystallization

Propionyl-CoA and butyryl-CoA were diluted in 20 mM HEPES pH 7.5 and stored at −20°C at a concentration of 20 mM as calculated using ∊260 nm = 16 400 M −1 cm−1. Purified human Gcn5L2 (amino acids 497–662) was mixed with each acyl-CoA to a final concentration of 1.6 mM acyl-CoA and 7.9 mg ml−1 protein. NaCl was added to a final concentration of 125 mM from a 5 M stock, and the resulting mixture was incubated on ice for 30 min. Both complexes were crystallized using hanging-drop vapor diffusion by mixing 1 µl protein–acyl-CoA complex solution with 1 µl well solution. Human Gcn5L2 bound to propionyl-CoA was crystallized in 20%(v/v) ethanol, 100 mM Tris pH 9.0. Human Gcn5L2 bound to butyryl-CoA was crystallized in 10%(v/v) 2-propanol, 3% glycerol, 100 mM HEPES pH 7.8, 11%(w/v) PEG 4000. Crystals were cryoprotected by soaking in well solution supplemented with 9% sucrose, 4% glucose, 8% ethylene glycol and 8% glycerol. Prior to data collection, crystals were flash-cooled in a liquid-nitrogen stream.

Data collection and processing

Diffraction data were collected using a Rigaku FR-E SuperBright X-ray generator at a wavelength of 1.54 Å and were recorded with a Saturn 944+ CCD detector. The data were processed with HKL-2000 (Otwinowski & Minor, 1997 ▸). The structures were solved using molecular replacement with MOLREP (Vagin & Teplyakov, 1997 ▸, 2010 ▸) from the CCP4 suite (Winn et al., 2011 ▸) using the coordinates of human Gcn5L2 (PDB entry 1z4r; Schuetz et al., 2007 ▸) as a search model. Refinement was carried out using REFMAC5 from the CCP4 suite (Murshudov et al., 2011 ▸) and the graphics program Coot for model building (Emsley & Cowtan, 2004 ▸). Simulated-annealing OMIT maps were generated by removing either propionyl-CoA or butyryl-CoA from the refined structures, fitting acetyl-CoA into the ligand density and performing three rounds of refinement with PHENIX, including two cycles of simulated annealing (Adams et al., 2010 ▸). Data-collection and refinement statistics are shown in Table 1 ▸. R.m.s.d. calculations were performed using PDB­eFold from the EMBL–EBI website. Structure figures were generated with PyMOL v.1.7.4 (Schrödinger).

Table 1

Data-collection, refinement and model statistics

Values in parentheses are for the outer shell.

Structure	Human Gcn5 bound to propionyl-CoA	Human Gcn5 bound to butyryl-CoA
PDB code	5h84	5h86
Diffraction source	Rigaku FR-E SuperBright	Rigaku FR-E SuperBright
Wavelength (Å)	1.54	1.54
Temperature (K)	293	293
Detector	Rigaku Saturn 944+	Rigaku Saturn 944+
Space group	P61	P61
a, b, c (Å)	38.09, 38.09, 187.06	38.25, 38.25, 186.97
α, β, γ (°)	90.0, 90.0, 120.0	90.0, 90.0, 120.0
Resolution range (Å)	29.17–2.00 (2.07–2.00)	24.81–2.08 (2.15–2.08)
Total No. of reflections	51218 (3583)	58587 (2201)
No. of unique reflections	10102 (915)	9086 (819)
Completeness (%)	97.98 (92.71)	98.06 (87.88)
Multiplicity	5.1 (3.9)	6.4 (2.7)
〈I/σ(I)〉	13.91 (5.68)	16.86 (5.39)
R meas	0.09651	0.08321
Overall B factor from Wilson plot (Å2)	18.80	19.72
Final R cryst (%)	17.6	16.4
Final R free (%)	20.2	20.7
CC1/2	0.996 (0.898)	0.997 (0.942)
CC*	0.999 (0.973)	0.999 (0.985)
No. of non-H atoms
Total	1507	1485
Protein	1334	1340
Ligand	68	57
Water	105	88
R.m.s. deviations
Bonds (Å)	1.22	1.25
Angles (°)	0.013	0.007
Average B factors (Å2)
Protein	19.30	19.70
Ligand	24.30	20.70
Water	29.60	28.60

PDB accession codes

Structures and amplitudes have been deposited in the Protein Data Bank with accession codes 5h84 (propionyl-CoA complex) and 5h86 (butyryl-CoA complex).

Results

Gcn5 is a weak acyltransferase

Previous studies of the P/CAF acetyltransferase, the catalytic domain of which shares 95% sequence identity with Gcn5, showed that P/CAF catalyzes histone propionylation with similar kinetics to acetylation (Leemhuis et al., 2008 ▸). Compared with acetyl-CoA, the K m of P/CAF for propionyl-CoA is only fourfold weaker, corresponding to a sixfold decrease in catalytic efficiency (Leemhuis et al., 2008 ▸). To determine whether human Gcn5 can similarly use other acyl-CoAs as a cofactor, we measured Gcn5 activity in the presence of either propionyl-CoA or butyryl-CoA and histone H3 peptide. We found that human Gcn5L2 efficiently acetylates and propionylates peptides, while its butyrylating activity is nearly undetectable (Fig. 1 ▸). Under these experimental conditions, Gcn5L2 propionylates histone peptides approximately ninefold more slowly and butyrylates peptides nearly 400-fold more slowly compared with its acetyltransferase activity (Fig. 1 ▸). Based on these relative rate measurements, Gcn5L2 is unlikely to contribute significantly to lysine butyrylation in vivo but may be capable of catalyzing lysine propionylation under physiological conditions.

Figure 1

Acyltransferase activity of hsGcn5L2. Rates of catalysis by hsGcn5L2 (10 µM) were measured using different acyl-CoA cofactors (500 µM) and an N-terminal histone H3 peptide (250 µM) containing the sequence ARTKQTARKSTGGKAPRKQLA.

Structures of hsGcn5L2 bound to propionyl-CoA and butyryl-CoA

To elucidate the structural basis for the ability of Gcn5L2 to discriminate among different acyl-CoA cofactors, we determined the structures of human Gcn5L2 bound to propionyl-CoA and butyryl-CoA to 2.0 and 2.1 Å resolution, respectively. Refinement statistics for each structure are summarized in Table 1 ▸. Simulated-annealing OMIT maps show clear density corresponding to the extra methyl group for propionyl-CoA (Fig. 2 ▸ a) or the extra ethyl chain for butyryl-CoA (Fig. 2 ▸ b). Compared with the structure of human Gcn5L2 bound to acetyl-CoA (Schuetz et al., 2007 ▸), the structures reported here are very similar; the root-mean-square difference (r.m.s.d.) in Cα positions is 0.13 Å for the structure of human Gcn5L2 in complex with propionyl-CoA and 0.24 Å for its structure in complex with butyryl-CoA.

Figure 2

Simulated-annealing OMIT maps demonstrate clear density for the acyl cofactors. OMIT maps were calculated with either the (a) propionyl or (b) butyryl moieties removed. 2F o − F c maps are contoured at 1σ (gray) and simulated-annealing OMIT maps are contoured at 2.5–3σ (magenta).

The active site of Gcn5 contains three features that facilitate transfer of the acyl chain to lysine: an active-site glutamate that functions as a general base (Tanner et al., 1999 ▸), a structurally conserved water molecule that forms a proton wire between the general base and the incoming lysine (Rojas et al., 1999 ▸) and residues that stabilize the position of the acyl-CoA (Figs. 3 ▸ a and 3 ▸ b; Rojas et al., 1999 ▸; Schuetz et al., 2007 ▸). This active-site geometry is preserved in the two acyl-CoA-bound structures reported here, including the orientation of the acyl-CoA thioester, which is coordinated by the backbone amide of Cys579, and the position of the water molecule, which is hydrogen-bonded to Glu575 (Figs. 3 ▸ c and 3 ▸ d). The conservation of the active-site geometry rules out the possibility that butyryl-CoA binding slows down Gcn5 catalytic activity by misaligning the active-site residues. The pantetheine arm and adenine moieties of coenzyme A superimpose well between all three acyl-CoA molecules; what differs is the respective position of the acetyl, propionyl and butyryl chains (Fig. 3 ▸ e). Although the positions of the C2 atoms in all three acyl-CoA molecules are the same (Figs. 3 ▸ f, 3 ▸ g and 3 ▸ h), the torsion angle formed between the S—C1 and C2—C3 bonds in propionyl-CoA is 24.5° (Fig. 3 ▸ i), compared with −61° for butyryl-CoA (Fig. 3 ▸ j). Whereas the C3 atom in propionyl-CoA fits within the active-site cleft of human Gcn5 (Fig. 3 ▸ c), butyryl-CoA binds in an orientation that places the terminal methyl group (C4) facing the solvent, since the catalytic water molecule blocks it from occupying the Gcn5 active-site cleft (Fig. 3 ▸ d). Although longer acyl chains could also bind in this orientation, we note that molecules such as crotonyl-CoA, which contain unsaturated carbon–carbon bonds that do not freely rotate, cannot adopt conformations that are compatible with this geometry.

Figure 3

The position of the acyl chain varies in the Gcn5 active site. (a) Overall structure of the catalytic domain of hsGcn5L2, shown in cartoon representation, bound to propionyl-CoA (cyan). The catalytic site is outlined with a black rectangle, where the catalytic water molecule is shown as a blue sphere and the acyl-CoA is depicted in stick representation. Close-up views of the active site of hsGcn5L2 bound to (b) acetyl-CoA (green), (c) propionyl-CoA (cyan) or (d) butyryl-CoA (orange). (e) Structural alignment of the three acyl-CoA molecules in the Gcn5 active site. (f, g, h) Close-up views of the different acyl groups: (f) acetyl-CoA, (g) propionyl-CoA and (h) butyryl-CoA. (i, j) Torsion angles adopted by the (i) propionyl and (j) butyryl moieties.

Model of the ternary complex with peptide and CoA

Since Gcn5 uses a direct-transfer mechanism to catalyze lysine acetylation (Tanner, Langer, Kim et al., 2000 ▸), Gcn5 must bind to the acyl-CoA cofactor and the peptide substrate at the same time. To determine whether the conformations adopted by propionyl-CoA and butyryl-CoA in complex with Gcn5L2 are compatible with peptide binding, models of human Gcn5L2 bound to each acyl-CoA molecule and a histone peptide (Fig. 4 ▸ a) were generated based on the structure of Tetrahymena Gcn5 (ttGcn5) bound to a bisubstrate analog consisting of CoA covalently linked to a histone peptide (PDB entry 1m1d; Poux et al., 2002 ▸). In our model of human Gcn5L2 bound to acetyl-CoA and peptide, the incoming lysine residue makes an angle of 105° with the acetyl thioester (Fig. 4 ▸ b), which is a reasonable angle of attack for a carbonyl group by a nucleophile (Bürgi et al., 1974 ▸). Propionyl-CoA also adopts a conformation compatible with this angle of attack, as the position of the terminal methyl group is in the same plane as the thioester, which leaves the lysine attack trajectory open (Fig. 4 ▸ c). By contrast, the terminal methyl of butyryl-CoA projects into the channel occupied by the lysine (Fig. 4 ▸ d). The Gcn5L2 active site cannot accommodate butyryl-CoA without ejecting the catalytic water molecule, so the butyryl chain sterically clashes with the incoming lysine. This explains why Gcn5L2 is a poor butyryltransferase, as the acyl chain, catalytic water molecule (Fig. 3 ▸ c) and incoming lysine (Fig. 4 ▸ d) cannot all fit into its active site.

Figure 4

Model of the ternary complex between hsGcn5L2, different acyl-CoA molecules and peptide. (a) Overall model with hsGn5L2 colored purple, the peptide colored yellow and acetyl-CoA colored green. The catalytic water molecule is depicted as a blue sphere. (b, c, d) Close-up views of the arrangement between the incoming lysine (yellow) and (b) acetyl-CoA (green), (c) propionyl-CoA (cyan) or (d) butyryl-CoA (orange).

Butyryl-CoA is a competitive inhibitor of acetylation by human Gcn5

Our results suggest that a naturally occurring acyl-CoA molecule, such as butyryl-CoA, could inhibit Gcn5 activity by binding to the enzyme in a way that prevents lysine from entering its active site. Since butyryl-CoA is a poor substrate for Gcn5 (Fig. 1 ▸) but is still able to bind in the active site (Fig. 3 ▸ d), we wondered whether it might act as a competitive inhibitor versus acetyl-CoA. To test this idea, we measured acetylation rates as a function of acetyl-CoA concentration in the presence of increasing concentrations of butyryl-CoA. As shown in Fig. 5 ▸(a), Gcn5 is robustly acetylates histone peptides under conditions where butyrylation is nearly undetectable. Fitting our initial velocity measurements to the Michaelis–Menten equation, we determine a K m for acetyl-CoA of 0.91 ± 0.09 µM (Fig. 5 ▸ a), which is comparable to previously reported K m values for yeast Gcn5, human Gcn5 and human P/CAF (Poux et al., 2002 ▸; Tanner, Langer, Kim et al., 2000 ▸; Tanner, Langer & Denu, 2000 ▸; Langer et al., 2002 ▸). We next measured acetylation kinetics in the presence of increasing concentrations of butyryl-CoA and fitted the resulting curves to either competitive, noncompetitive or uncompetitive inhibition models. Competitive inhibition clearly fits the data best, as the sum of the squares of the residuals normalized to the degrees of freedom is 0.053 for the competitive model, compared with 0.24 and 0.26 for noncompetitive and uncompetitive inhibition, respectively. We measured an inhibition constant (K i) of 5.6 ± 0.7 µM from our global fit to a competitive-inhibition model (Fig. 5 ▸ b). Taken together with our structural findings (Figs. 4 ▸ a and 4 ▸ d), these data indicate that butyryl-CoA competitively inhibits acetylation by Gcn5 by binding to the free form of the enzyme and preventing acyl-chain transfer.

Figure 5

Butyryl-CoA competitively inhibits acetylation by human Gcn5. (a) Steady-state kinetic analysis comparing acetylation and butyrylation by human Gcn5 using a fixed concentration of 300 µM H3 peptide. By fitting this curve to the Michaelis–Menten equation, we obtain K m = 1.05 ± 0.02 µM and k cat = 0.91 ± 0.09 s−1. (b) Acetylation kinetics monitored in the presence of increasing concentrations of butyryl-CoA (0–0.3 mM). Data were globally fitted to a competitive-inhibition model.

Discussion

We have determined crystal structures that describe how Gcn5 accommodates propionyl-CoA in its active site and provide a structural mechanism that explains our biochemical data showing that human Gcn5 discriminates between different acyl-CoA molecules. Since unsaturated acyl chains greater than three C atoms in length (propyl groups) cannot fit into the active site of Gcn5, butyryl-CoA binds in a conformation that is incompatible with catalysis. The butyryl-CoA C3 and C4 atoms occupy the channel for the incoming lysine (Fig. 3 ▸ d), which prevents the peptide substrate from accessing the active-site cleft of Gcn5. We further show that butyryl-CoA is a competitive inhibitor versus acetyl-CoA for human Gcn5 (Fig. 5 ▸ c), raising the question as to whether fluctuating levels of acyl-CoA molecules in cells may regulate the activity of Gcn5.

Coenzyme A is a common nucleotide cofactor that carries many different kinds of acyl groups in vivo (King & Reiss, 1985 ▸), and many metabolic processes produce or consume acyl-CoA (Albaugh et al., 2011 ▸). As a result, the intracellular concentrations of different acyl-CoA species change in response to metabolic fluctuations (Hosokawa et al., 1986 ▸; Palladino et al., 2012 ▸; King & Reiss, 1985 ▸). For example, measurements of the intracellular concentration of acetyl-CoA vary based on nutrient availability, and range from 3 to 30 µM in yeast (Cai et al., 2011 ▸; Weinert et al., 2014 ▸) and from 2 to 13 µM in human cells (Lee et al., 2014 ▸). With a K m for acetyl-CoA of 0.91 ± 0.09 µM (Fig. 5 ▸ a), acetyl-CoA availability may regulate the activity of human Gcn5 (Albaugh et al., 2011 ▸). Consistent with this, Gcn5-catalyzed histone acetylation is induced under growth conditions with high intracellular levels of acetyl-CoA (Cai et al., 2011 ▸). It is not yet known whether the intracellular concentrations of other acyl-CoA species are sufficiently high to impact acetylation by KATs such as Gcn5. Although studies quantifying absolute concentrations of propionyl-CoA and butyryl-CoA in cells have not been performed, measurements of the relative abundance of different acyl-CoAs in fasting rat (King & Reiss, 1985 ▸) and mouse (Palladino et al., 2012 ▸) liver found roughly 4:2:1 molar ratios of acetyl-CoA, propionyl-CoA and butyryl-CoA. With an inhibition constant of 5.6 ± 0.7 µM for butyryl-CoA, it is possible that intracellular acyl-CoA ratios could regulate the activity of Gcn5. As a result, the activity of Gcn5 would be sensitive to metabolic flux, as the relative amounts of different acyl-CoA species change in response to metabolic activity (Hosokawa et al., 1986 ▸; Palladino et al., 2012 ▸; King & Reiss, 1985 ▸).

Other CoA-based molecules have been implicated as acetyltransferase inhibitors in vivo and in vitro. Free CoA is a potent competitive inhibitor of yeast Gcn5 (Tanner, Langer, Kim et al., 2000 ▸) and human P/CAF (Tanner, Langer & Denu, 2000 ▸) in vitro, with inhibition constants (K i) of 6.7 and 0.44 µM, respectively. Combined with the observation that free CoA is present at roughly equimolar concentrations to acetyl-CoA in cells (Gao et al., 2007 ▸; Lee et al., 2014 ▸), it is plausible that the ratio of acetyl-CoA to CoA may modulate KAT activity (Albaugh et al., 2011 ▸). Interestingly, a recent study profiling the acyl-chain specificity of Gcn5 observed potent inhibition by long fatty acyl-CoA molecules such as palmitoyl-CoA (Montgomery et al., 2015 ▸), further supporting the idea that acyl-CoA molecules may function as natural KAT inhibitors. Although relatively few synthetic inhibitors for KATs have been developed, some of the most potent compounds exploit CoA-based scaffolds with structural complementarity to the active site (Furdas et al., 2012 ▸). Bisubstrate analogues comprised of CoA–peptide conjugates mimic the ternary complex and inhibit Gcn5 at micromolar concentrations (Poux et al., 2002 ▸). In light of these observations, the structures presented here suggest that unsaturated acyl-CoAs may well act as natural acetyltransferase inhibitors in vivo and provide a clue as to how other CoA-based scaffolds may be exploited to design future generations of acetyltransferase inhibitors.

PDB reference: human Gcn5L2, bound to propionyl-CoA, 5h84

PDB reference: bound to butyryl-CoA, 5h86