Jun Cheng1, Xiaoping Qi2, Dawei Chen2, Xujian Xu3, Guozheng Wang4, Yuzhu Dai4, Dawei Cui5, Qingyong Chen2, Ping Fan6, Liuda Ni7, Miao Liu8, Feiyan Zhu9, Mei Yang10, Changjun Wang11, Yuexi Li12, Changgui Sun4, Zhongyong Wang13. 1. Department of Clinical Laboratory, The First Affiliated Hospital of Wenzhou Medical UniversityWenzhou, China; Department of Clinical Laboratory Science, The 117th Hospital of PLAHangzhou,China. 2. Department of Respiratory Medicine, The 117th Hospital of PLA Hangzhou, China. 3. Department of Biotechnology, The University of Tokyo Tokyo, Japan. 4. Department of Clinical Laboratory Science, The 117th Hospital of PLA Hangzhou,China. 5. State Key Laboratory for Diagnosis and Treatment of Infectious Diseases, First Affiliated Hospital, College of Medicine, Zhejiang University Hangzhou, China. 6. Department of Infectious Diseases, The 113rd Hospital of PLA Ningbo, China. 7. Department of Infectious Diseases, The 85th Hospital of PLA Shanghai, China. 8. Department of Radio Diagnosis and Imaging, The 117th Hospital of PLA Hangzhou, China. 9. Department of Infectious Diseases, The 117th Hospital of PLA Hangzhou, China. 10. Department of Epidemiology and Infection Control, The 117th Hospital of PLA Hangzhou, China. 11. Institute of Military Medical Sciences of Nanjing Military Command Nanjing, China. 12. Center for Disease Control and Prevention of Nanjing Military Command Nanjing, China. 13. Department of Clinical Laboratory, The First Affiliated Hospital of Wenzhou Medical University Wenzhou, China.
Abstract
BACKGROUND: Human adenovirus type 7 (HAdV7) is globally attracting great concern as its high morbidity and severity in respiratory diseases, especially in Asia. OBJECTIVE: To investigate the clinical and epidemiologic characteristics of HAdV7 infection outbreak in East China. METHODS: The clinical samples were collected from the patients of an ARD outbreak in East Chinafor the detection of causative pathogens by multiplex PCR. The molecular type of human adenovirus isolates were identified by sequencing and homologous comparison based on their hexon genes. The spatiotemporal dynamics of global HAdV7 was investigated using the phylogenetic and phylogeographic analyses. Total 67 referenced HAdV7 hexon sequences (>800 bp) from GenBank were selected for constructing the maximum likelihood tree by MEGA 5.1.0, grouped according to the tree topology for the further migration analysis by PAUP* 4.0 and MigraPhyla 1.0 b to understand the transmission patterns of HAdV7 in global epidemics. RESULTS: The results showed HAdV7 as the causative pathogen in this outbreak, and the outbreak strains had the hexon sequences highly identical with the isolates in Shaanxi (2012). The origin of HAdV7 was inferred as California, meanwhile a total of 21 migration routes were acquired. HAdV7 in this outbreak was statistically proven dispersed from Shaanxi province (2012). CONCLUSIONS: The analyses of epidemiology and transmission pattern of HAdV7 would not only enrich the molecular biological basic database but also provide theoretical basis for HAdV7 prevention and control strategy.
BACKGROUND:Human adenovirus type 7 (HAdV7) is globally attracting great concern as its high morbidity and severity in respiratory diseases, especially in Asia. OBJECTIVE: To investigate the clinical and epidemiologic characteristics of HAdV7 infection outbreak in East China. METHODS: The clinical samples were collected from the patients of an ARD outbreak in East Chinafor the detection of causative pathogens by multiplex PCR. The molecular type of human adenovirus isolates were identified by sequencing and homologous comparison based on their hexon genes. The spatiotemporal dynamics of global HAdV7 was investigated using the phylogenetic and phylogeographic analyses. Total 67 referenced HAdV7 hexon sequences (>800 bp) from GenBank were selected for constructing the maximum likelihood tree by MEGA 5.1.0, grouped according to the tree topology for the further migration analysis by PAUP* 4.0 and MigraPhyla 1.0 b to understand the transmission patterns of HAdV7 in global epidemics. RESULTS: The results showed HAdV7 as the causative pathogen in this outbreak, and the outbreak strains had the hexon sequences highly identical with the isolates in Shaanxi (2012). The origin of HAdV7 was inferred as California, meanwhile a total of 21 migration routes were acquired. HAdV7 in this outbreak was statistically proven dispersed from Shaanxi province (2012). CONCLUSIONS: The analyses of epidemiology and transmission pattern of HAdV7 would not only enrich the molecular biological basic database but also provide theoretical basis for HAdV7 prevention and control strategy.
Entities:
Keywords:
Human adenovirus type 7; clinical characteristics; epidemiology; phylogeny; phylogeography
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