Literature DB >> 2732096

Cellular localization of induced human interferon-beta mRNA by non-radioactive in situ hybridization.

H Multhaupt1, G Gross, P Fritz, K Köhler.   

Abstract

Induced interferon-beta (IFN-beta) mRNA was localized in human FS-4 fibroblasts by in situ hybridization using biotinylated probes. The hybridization sites were detected by incubation with a nick-translated genomic DNA probe (1.8 kb) via streptavidin-colloidal gold followed by silver contrast enhancement. The positive signals were observed by reflection-contrast light microscopy. IFN-beta mRNA was transiently induced by poly r(I): r(C) in fibroblasts 2-4 h after induction. Induction in the presence of cycloheximide and actinomycin D (superinduction conditions) exhibited an enhanced level of IFN-beta mRNA with a maximum at 4-8 h. The kinetics of the IFN-beta mRNA expression in the cytoplasm as revealed by in situ hybridization proved to be compatible with the results of Northern blotting experiments of total cellular RNA.

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Year:  1989        PMID： 2732096     DOI： 10.1007/bf00493007

Source DB:  PubMed          Journal:  Histochemistry        ISSN： 0301-5564


  27 in total

1.  Acetylation of chromosome squashes of Drosophila melanogaster decreases the background in autoradiographs from hybridization with [125I]-labeled RNA.

Authors:  S Hayashi; I C Gillam; A D Delaney; G M Tener
Journal:  J Histochem Cytochem       Date:  1978-08       Impact factor: 2.479

2.  Light microscopical detection of single 5 and 20 nm gold particles used for immunolabelling of plasma membrane antigens with silver enhancement and reflection contrast.

Authors:  E C Hoefsmit; C Korn; N Blijleven; J S Ploem
Journal:  J Microsc       Date:  1986-08       Impact factor: 1.758

3.  Formation and detection of RNA-DNA hybrid molecules in cytological preparations.

Authors:  J G Gall; M L Pardue
Journal:  Proc Natl Acad Sci U S A       Date:  1969-06       Impact factor: 11.205

4.  Inducers of interferon and host resistance. II. Multistranded synthetic polynucleotide complexes.

Authors:  A K Field; A A Tytell; G P Lampson; M R Hilleman
Journal:  Proc Natl Acad Sci U S A       Date:  1967-09       Impact factor: 11.205

5.  Immunogold-silver staining: new method of immunostaining with enhanced sensitivity.

Authors:  C S Holgate; P Jackson; P N Cowen; C C Bird
Journal:  J Histochem Cytochem       Date:  1983-07       Impact factor: 2.479

6.  Analysis of interferon mRNA in human fibroblast cells induced to produce interferon.

Authors:  N B Raj; P M Pitha
Journal:  Proc Natl Acad Sci U S A       Date:  1981-12       Impact factor: 11.205

7.  A membrane-filter technique for the detection of complementary DNA.

Authors:  D T Denhardt
Journal:  Biochem Biophys Res Commun       Date:  1966-06-13       Impact factor: 3.575

8.  Visualization and quantitation of poly A+-mRNA by using in situ hybridization with biotinylated oligonucleotides.

Authors:  K Wehner; P Fritz; K Köhler; H Multhaupt
Journal:  Acta Histochem Suppl       Date:  1989

9.  Chromosomal localization of a unique gene by non-autoradiographic in situ hybridization.

Authors:  J E Landegent; N Jansen in de Wal; G J van Ommen; F Baas; J J de Vijlder; P van Duijn; M Van der Ploeg
Journal:  Nature       Date:  1985 Sep 12-18       Impact factor: 49.962

10.  Actin gene expression visualized in chicken muscle tissue culture by using in situ hybridization with a biotinated nucleotide analog.

Authors:  R H Singer; D C Ward
Journal:  Proc Natl Acad Sci U S A       Date:  1982-12       Impact factor: 11.205
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  3 in total

Review 1.  Cytochemical detection systems for in situ hybridization, and the combination with immunocytochemistry, 'who is still afraid of red, green and blue?'.

Authors:  E J Speel; F C Ramaekers; A H Hopman
Journal:  Histochem J       Date:  1995-11

2.  Expression of very low density lipoprotein receptor in the vascular wall. Analysis of human tissues by in situ hybridization and immunohistochemistry.

Authors:  H A Multhaupt; M E Gåfvels; K Kariko; H Jin; C Arenas-Elliot; B I Goldman; J F Strauss; B Angelin; M J Warhol; K R McCrae
Journal:  Am J Pathol       Date:  1996-06       Impact factor: 4.307

3.  In situ hybridization with digoxigenin-labeled probes: sensitive and reliable detection method applied to myelinating rat brain.

Authors:  H Breitschopf; G Suchanek; R M Gould; D R Colman; H Lassmann
Journal:  Acta Neuropathol       Date:  1992       Impact factor: 17.088
  3 in total

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