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Literature DB >> 6189883

Immunogold-silver staining: new method of immunostaining with enhanced sensitivity.

C S Holgate, P Jackson, P N Cowen, C C Bird.

Abstract

A new method for demonstrating antigens in paraffin sections of formol sublimate-fixed tissue is described that utilizes an "indirect" immunohistological technique employing immunoglobulin adsorbed to colloidal gold as the secondary antiserum. The gold particles introduced to antigenic sites are revealed by a silver precipitation reaction. This technique, the immunogold-silver staining method, is of much enhanced sensitivity (up to 200-fold) as compared with standard immunoperoxidase and immunogold staining methods. The results have been confirmed in a study of immunoglobulins in reactive human tonsil. The use of this new method for double immunolabeling is also described.

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Year: 1983 PMID： 6189883 DOI： 10.1177/31.7.6189883

Source DB: PubMed Journal: J Histochem Cytochem ISSN： 0022-1554 Impact factor: 2.479

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Cited

114 in total

1. Relationship between the clinical features and the viral antigen in the extremity blood of the patients with hemorrhagic fever with renal syndrome.

Authors: C Han; L Zeng; D Luo
Journal: J Tongji Med Univ Date: 1999

7. Combination of the peroxidase anti-peroxidase (PAP)- and avidin-biotin-peroxidase complex (ABC)-techniques: an amplification alternative in immunocytochemical staining.

Authors: M Davidoff; W Schulze
Journal: Histochemistry Date: 1990

Review 8. Notes on the application of microwaves in histopathology.

Authors: A J Suurmeijer; M E Boon; L P Kok
Journal: Histochem J Date: 1990 Jun-Jul

9. Detection of human papilloma viruses in paraffin wax sections with biotinylated synthetic oligonucleotide probes and immunogold staining.

Authors: H A Cubie; M Norval
Journal: J Clin Pathol Date: 1989-09 Impact factor: 3.411

10. Contact-dependent transfer of the galactose-specific lectin of Entamoeba histolytica to the lateral surface of enterocytes in culture.

Authors: A Leroy; G De Bruyne; M Mareel; C Nokkaew; G Bailey; H Nelis
Journal: Infect Immun Date: 1995-11 Impact factor: 3.441

Immunogold-silver staining: new method of immunostaining with enhanced sensitivity.

1. Relationship between the clinical features and the viral antigen in the extremity blood of the patients with hemorrhagic fever with renal syndrome.

Review 2. Effects of dissolucytotic gold ions on recovering brain lesions.

3. Localization of arginine-vasopressin in endothelial cells of rat pulmonary artery.

4. Fibre typing using sarcoplasmic reticulum Ca2+-ATPase and myoglobin immunohistochemistry in rat gastrocnemius muscle.

5. Autometallographic silver-enhancement of colloidal gold particles used to label phagocytic cells.

6. A double protein A-gold-silver staining method for tissue antigens in light microscopy.

7. Combination of the peroxidase anti-peroxidase (PAP)- and avidin-biotin-peroxidase complex (ABC)-techniques: an amplification alternative in immunocytochemical staining.

Review 8. Notes on the application of microwaves in histopathology.

9. Detection of human papilloma viruses in paraffin wax sections with biotinylated synthetic oligonucleotide probes and immunogold staining.

10. Contact-dependent transfer of the galactose-specific lectin of Entamoeba histolytica to the lateral surface of enterocytes in culture.