Literature DB >> 27307197

Broadening substrate specificity of a chain-extending ketosynthase through a single active-site mutation.

Annabel C Murphy1, Hui Hong1, Steve Vance2, R William Broadhurst1, Peter F Leadlay1.   

Abstract

An in vitro model system based on a ketosynthase domain of the erythromycin polyketide synthase was used to probe the apparent substrate tolerance of ketosynthase domains of the mycolactone polyketide synthase. A specific residue change was identified that led to an emphatic increase in turnover of a range of substrates.

Mesh：

Substances：

Year:  2016        PMID： 27307197     DOI： 10.1039/c6cc03501a

Source DB:  PubMed          Journal:  Chem Commun (Camb)        ISSN： 1359-7345            Impact factor:   6.222


  11 in total

Review 1.  Engineering modular polyketide synthases for production of biofuels and industrial chemicals.

Authors:  Wenlong Cai; Wenjun Zhang
Journal:  Curr Opin Biotechnol       Date:  2017-09-22       Impact factor: 9.740

2.  Synthetic biology of polyketide synthases.

Authors:  Satoshi Yuzawa; Tyler W H Backman; Jay D Keasling; Leonard Katz
Journal:  J Ind Microbiol Biotechnol       Date:  2018-02-09       Impact factor: 3.346

3.  Preparative production of an enantiomeric pair by engineered polyketide synthases.

Authors:  Takeshi Miyazawa; Brendan J Fitzgerald; Adrian T Keatinge-Clay
Journal:  Chem Commun (Camb)       Date:  2021-08-11       Impact factor: 6.065

4.  Structure and mechanistic analyses of the gating mechanism of elongating ketosynthases.

Authors:  Jeffrey T Mindrebo; Aochiu Chen; Woojoo E Kim; Rebecca N Re; Tony D Davis; Joseph P Noel; Michael D Burkart
Journal:  ACS Catal       Date:  2021-05-26       Impact factor: 13.700

5.  Engineering of Chimeric Polyketide Synthases Using SYNZIP Docking Domains.

Authors:  Maja Klaus; Alicia D D'Souza; Aleksandra Nivina; Chaitan Khosla; Martin Grininger
Journal:  ACS Chem Biol       Date:  2019-02-11       Impact factor: 5.100

6.  ClusterCAD: a computational platform for type I modular polyketide synthase design.

Authors:  Clara H Eng; Tyler W H Backman; Constance B Bailey; Christophe Magnan; Héctor García Martín; Leonard Katz; Pierre Baldi; Jay D Keasling
Journal:  Nucleic Acids Res       Date:  2018-01-04       Impact factor: 16.971

Review 7.  Strategies in megasynthase engineering - fatty acid synthases (FAS) as model proteins.

Authors:  Manuel Fischer; Martin Grininger
Journal:  Beilstein J Org Chem       Date:  2017-06-21       Impact factor: 2.883

Review 8.  Towards Precision Engineering of Canonical Polyketide Synthase Domains: Recent Advances and Future Prospects.

Authors:  Carmen L Bayly; Vikramaditya G Yadav
Journal:  Molecules       Date:  2017-02-05       Impact factor: 4.411

Review 9.  Engineering enzymatic assembly lines to produce new antibiotics.

Authors:  Kenan Aj Bozhüyük; Jason Micklefield; Barrie Wilkinson
Journal:  Curr Opin Microbiol       Date:  2019-11-16       Impact factor: 7.934

10.  Biosynthetic engineering of the antifungal, anti-MRSA auroramycin.

Authors:  Wan Lin Yeo; Elena Heng; Lee Ling Tan; Yi Wee Lim; Kuan Chieh Ching; De-Juin Tsai; Yi Wun Jhang; Tsai-Ling Lauderdale; Kak-Shan Shia; Huimin Zhao; Ee Lui Ang; Mingzi M Zhang; Yee Hwee Lim; Fong T Wong
Journal:  Microb Cell Fact       Date:  2020-01-06       Impact factor: 5.328
View more

北京卡尤迪生物科技股份有限公司 © 2022-2023.