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Literature DB >> 34378565

Preparative production of an enantiomeric pair by engineered polyketide synthases.

Takeshi Miyazawa¹, Brendan J Fitzgerald, Adrian T Keatinge-Clay.

Abstract

Using the updated module boundary of polyketide assembly lines, modules from the pikromycin synthase were recombined into engineered synthases that furnish an enantiomeric pair of 2-stereocenter triketide lactones at >99% ee with yields up to 0.39 g per liter of E. coli K207-3 in shake flasks.

Entities: Chemical

Year: 2021 PMID： 34378565 PMCID： PMC8410653 DOI： 10.1039/d1cc03073f

Source DB: PubMed Journal: Chem Commun (Camb) ISSN： 1359-7345 Impact factor: 6.065

39 in total

Review 1. Combinatorial biosynthesis of reduced polyketides.

Authors: Kira J Weissman; Peter F Leadlay
Journal: Nat Rev Microbiol Date: 2005-12 Impact factor: 60.633

2. Enzymatic extender unit generation for in vitro polyketide synthase reactions: structural and functional showcasing of Streptomyces coelicolor MatB.

Authors: Amanda J Hughes; Adrian Keatinge-Clay
Journal: Chem Biol Date: 2011-02-25

3. Structural basis for binding specificity between subclasses of modular polyketide synthase docking domains.

Authors: Tonia J Buchholz; Todd W Geders; Frank E Bartley; Kevin A Reynolds; Janet L Smith; David H Sherman
Journal: ACS Chem Biol Date: 2009-01-16 Impact factor: 5.100

4. Metabolic engineering of Escherichia coli for improved 6-deoxyerythronolide B production.

Authors: Sumati Murli; Jonathan Kennedy; Linda C Dayem; John R Carney; James T Kealey
Journal: J Ind Microbiol Biotechnol Date: 2003-07-26 Impact factor: 3.346

5. Development of a high cell-density fed-batch bioprocess for the heterologous production of 6-deoxyerythronolide B in Escherichia coli.

Authors: Janice Lau; Carnie Tran; Peter Licari; Jorge Galazzo
Journal: J Biotechnol Date: 2004-05-13 Impact factor: 3.307

Preparative production of an enantiomeric pair by engineered polyketide synthases.

Review 1. Combinatorial biosynthesis of reduced polyketides.

2. Enzymatic extender unit generation for in vitro polyketide synthase reactions: structural and functional showcasing of Streptomyces coelicolor MatB.

3. Structural basis for binding specificity between subclasses of modular polyketide synthase docking domains.

4. Metabolic engineering of Escherichia coli for improved 6-deoxyerythronolide B production.

5. Development of a high cell-density fed-batch bioprocess for the heterologous production of 6-deoxyerythronolide B in Escherichia coli.

6. Complete Reconstitution and Deorphanization of the 3 MDa Nocardiosis-Associated Polyketide Synthase.

7. Ketosynthase Domain Constrains the Design of Polyketide Synthases.

8. Structural basis for the biosynthesis of lovastatin.

9. Deciphering the genetic basis for polyketide variation among mycobacteria producing mycolactones.

10. An in vitro platform for engineering and harnessing modular polyketide synthases.

1. Priming enzymes from the pikromycin synthase reveal how assembly-line ketosynthases catalyze carbon-carbon chemistry.

2. Metabolic pathway assembly using docking domains from type I cis-AT polyketide synthases.

3. Engineering Aspergillus oryzae for the Heterologous Expression of a Bacterial Modular Polyketide Synthase.