| Literature DB >> 27285672 |
Zhiheng Huang1, Chen Nan2, Hanqing Wang3, Qiang Su1, Wenda Xue1, Yanyan Chen1, Xin Shan1, Jinao Duan4, Gang Chen1, Weiwei Tao5.
Abstract
Crocetin ester (CE) is the active ingredient of Crocus sativus L. stigmas and Gardenia jasminoides Ellis fruit. The main purpose of the present study was to investigate the protective effect of CE on isoproterenol (ISO)-induced acute myocardial ischemia (AMI) through Rho/ROCK/NF-κB pathway and explore its underlying mechanism. Administration of CE (25 and 50mg/kg) could significantly reduce the serum contents of pro-inflammatory cytokines including tumor necrosis factor-alpha (TNF-α), interleukin-1 beta (IL-1β) and interleukin-6 (IL-6). In addition, pretreatment with CE attenuated the contents of creatine kinase (CK), malondialdehyde (MDA) and the activities of lactate dehydrogenase (LDH), superoxide dismutase (SOD) in serum. Treatment with CE also improved the histopathological alteration and decreased the ST elevation. Furthermore, CE could ameliorate the cardiac expressions of Cu, Zn-superoxide dismutase (SOD1), MDA5, Rho, ROCK, p-IκB and p-NF-κBp65 in ISO-induced rats. It was assumed that CE might be a new therapeutic candidate for the treatment of AMI possibly through the inhibition of Rho/ROCK/NF-κB pathway.Entities:
Keywords: Crocetin ester; Myocardial ischemia; NF-κB; Rho
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Year: 2016 PMID： 27285672 DOI： 10.1016/j.intimp.2016.05.025
Source DB: PubMed Journal: Int Immunopharmacol ISSN： 1567-5769 Impact factor: 4.932