Mitra Foroutan-Ghaznavi1,2,3, Seyed-Mohammad Mazloomi4,5, Vahid Montazeri6,7, Saeed Pirouzpanah8,9. 1. Department of Clinical Nutrition, Faculty of Nutrition and Food Sciences, Shiraz University of Medical Sciences, 7134814336, Shiraz, Iran. 2. Stem Cell Research Center, Tabriz University of Medical Sciences, 5166614756, Tabriz, Iran. 3. Department of Clinical Nutrition, Shahid Rajaee (Emtiaz) Trauma Hospital, Shiraz University of Medical Sciences, 7194815711, Shiraz, Iran. 4. Nutrition Research Center, Shiraz University of Medical Sciences, 7193635899, Shiraz, Iran. 5. Department of Food Hygiene and Quality Control, Faculty of Nutrition and Food Sciences, Shiraz University of Medical Sciences, 7134814336, Shiraz, Iran. 6. Department of Thoracic Surgery, Faculty of Medicine, Tabriz University of Medical Sciences, 5166414766, Tabriz, Iran. 7. Department of Surgery, Nour-Nejat Hospital, 5138665793, Tabriz, Iran. 8. Stem Cell Research Center, Tabriz University of Medical Sciences, 5166614756, Tabriz, Iran. pirouzpanah@gmail.com. 9. Molecular Medicine Research Center, Biomedicine Institute, Tabriz University of Medical Sciences, 5166414766, Tabriz, Iran. pirouzpanah@gmail.com.
Abstract
PURPOSE: Metastasis is a major leading cause of mortality in female breast cancer (BrCa). Cellular motility is a pathological process of metastasis remarked by the overexpression of cortactin (CTTN), Ras homolog family member-A (RhoA), and Rho-associated kinase (ROCK) genes. Their balance is responsible for upholding the integrity of healthy epithelial cell junctions. This study aimed to explore the associations between a posteriori dietary patterns and the expression levels of pro-metastatic genes in primary BrCa. METHODS: In this consecutive case series, 215 eligible women, newly diagnosed with histologically confirmed non-metastatic BrCa (stage I-IIIA), were recruited from Hospitals in Tabriz, Northwestern Iran (2015-2017). The tumoral expression levels of genes were quantified using real-time reverse transcription-polymerase chain reaction. Dietary data assessment was carried out using a validated food frequency questionnaire. RESULTS: Three dietary patterns were identified using principal component analysis (KMO = 0.699). Adherence to the "vegan" pattern (vegetables, fruits, legumes, nuts, seeds, and whole grains) was inversely associated with the expression levels of RhoA (ORAdj.T3vs.T1 = 0.24, 95%CI 0.07-0.79) and ROCK (ORAdj.T3vs.T1 = 0.26, 95%CI 0.08-0.87). In addition, the highest adherence to the "prudent" pattern (spices, seafood, dairy, and vegetable oils) decreased the odds of overexpressions at RhoA (ORAdj.T3vs.T1 = 0.26, 95%CI 0.08-0.84) and ROCK genes (ORAdj.T3vs.T1 = 0.29, 95%CI 0.09-0.95). The highest adherence to "Western" pattern (meat, processed meat, hydrogenated fat, fast food, refined cereals, sweets, and soft drinks) was a risk factor associated with the overexpression of RhoA (ORAdj.T3vs.T1 = 3.15, 95%CI 1.12-8.85). CONCLUSION: Adherence to healthy dietary patterns was significantly associated with the downregulation of pro-metastatic genes. Findings provided new implications to advance the nutrigenomic knowledge to prevent the odds of over-regulations in pro-metastatic genes of the primary BrCa.
PURPOSE: Metastasis is a major leading cause of mortality in female breast cancer (BrCa). Cellular motility is a pathological process of metastasis remarked by the overexpression of cortactin (CTTN), Ras homolog family member-A (RhoA), and Rho-associated kinase (ROCK) genes. Their balance is responsible for upholding the integrity of healthy epithelial cell junctions. This study aimed to explore the associations between a posteriori dietary patterns and the expression levels of pro-metastatic genes in primary BrCa. METHODS: In this consecutive case series, 215 eligible women, newly diagnosed with histologically confirmed non-metastatic BrCa (stage I-IIIA), were recruited from Hospitals in Tabriz, Northwestern Iran (2015-2017). The tumoral expression levels of genes were quantified using real-time reverse transcription-polymerase chain reaction. Dietary data assessment was carried out using a validated food frequency questionnaire. RESULTS: Three dietary patterns were identified using principal component analysis (KMO = 0.699). Adherence to the "vegan" pattern (vegetables, fruits, legumes, nuts, seeds, and whole grains) was inversely associated with the expression levels of RhoA (ORAdj.T3vs.T1 = 0.24, 95%CI 0.07-0.79) and ROCK (ORAdj.T3vs.T1 = 0.26, 95%CI 0.08-0.87). In addition, the highest adherence to the "prudent" pattern (spices, seafood, dairy, and vegetable oils) decreased the odds of overexpressions at RhoA (ORAdj.T3vs.T1 = 0.26, 95%CI 0.08-0.84) and ROCK genes (ORAdj.T3vs.T1 = 0.29, 95%CI 0.09-0.95). The highest adherence to "Western" pattern (meat, processed meat, hydrogenated fat, fast food, refined cereals, sweets, and soft drinks) was a risk factor associated with the overexpression of RhoA (ORAdj.T3vs.T1 = 3.15, 95%CI 1.12-8.85). CONCLUSION: Adherence to healthy dietary patterns was significantly associated with the downregulation of pro-metastatic genes. Findings provided new implications to advance the nutrigenomic knowledge to prevent the odds of over-regulations in pro-metastatic genes of the primary BrCa.
Authors: Brian L Rothschild; Ann H Shim; Amanda Gatesman Ammer; Laura C Kelley; Kimberly B Irby; Julie A Head; Lin Chen; Marileila Varella-Garcia; Peter G Sacks; Barbara Frederick; David Raben; Scott A Weed Journal: Cancer Res Date: 2006-08-15 Impact factor: 12.701
Authors: Tomer Meirson; Alessandro Genna; Nikola Lukic; Tetiana Makhnii; Joel Alter; Ved P Sharma; Yarong Wang; Abraham O Samson; John S Condeelis; Hava Gil-Henn Journal: Oncotarget Date: 2018-04-24