Literature DB >> 27252380

1,2-Propanediol Dehydration in Roseburia inulinivorans: STRUCTURAL BASIS FOR SUBSTRATE AND ENANTIOMER SELECTIVITY.

Joseph W LaMattina1, Nicholas D Keul1, Pierre Reitzer1, Suraj Kapoor1, Felipe Galzerani2, Daniel J Koch2, Iuri E Gouvea2, William N Lanzilotta3.   

Abstract

Glycyl radical enzymes (GREs) represent a diverse superfamily of enzymes that utilize a radical mechanism to catalyze difficult, but often essential, chemical reactions. In this work we present the first biochemical and structural data for a GRE-type diol dehydratase from the organism Roseburia inulinivorans (RiDD). Despite high sequence (48% identity) and structural similarity to the GRE-type glycerol dehydratase from Clostridium butyricum, we demonstrate that the RiDD is in fact a diol dehydratase. In addition, the RiDD will utilize both (S)-1,2-propanediol and (R)-1,2-propanediol as a substrate, with an observed preference for the S enantiomer. Based on the new structural information we developed and successfully tested a hypothesis that explains the functional differences we observe.
© 2016 by The American Society for Biochemistry and Molecular Biology, Inc.

Entities:  

Keywords:  chemical biology; diol dehydratase; enzyme structure; glycerol dehydratase; glycyl radical enzyme; intestinal metabolism; metabolic engineering; radical; radical dehydration

Mesh：

Substances：

Year:  2016        PMID： 27252380      PMCID： PMC4957038          DOI： 10.1074/jbc.M116.721142

Source DB:  PubMed          Journal:  J Biol Chem        ISSN： 0021-9258            Impact factor:   5.157


  55 in total

Review 1.  Radical mechanisms of enzymatic catalysis.

Authors:  P A Frey
Journal:  Annu Rev Biochem       Date:  2001       Impact factor: 23.643

2.  Pyruvate formate-lyase, evidence for an open conformation favored in the presence of its activating enzyme.

Authors:  Yi Peng; Susan E Veneziano; Gregory D Gillispie; Joan B Broderick
Journal:  J Biol Chem       Date:  2010-06-22       Impact factor: 5.157

3.  Structure and mechanism of the glycyl radical enzyme pyruvate formate-lyase.

Authors:  A Becker; K Fritz-Wolf; W Kabsch; J Knappe; S Schultz; A F Volker Wagner
Journal:  Nat Struct Biol       Date:  1999-10

4.  A spectrophotometric method for the direct recording of dioldehydrase activity.

Authors:  G L Sottocasa; N Stagni; B De Bernard
Journal:  Experientia       Date:  1971-10-15

5.  Structural basis for a Kolbe-type decarboxylation catalyzed by a glycyl radical enzyme.

Authors:  Berta M Martins; Martin Blaser; Mikolaj Feliks; G Matthias Ullmann; Wolfgang Buckel; Thorsten Selmer
Journal:  J Am Chem Soc       Date:  2011-08-26       Impact factor: 15.419

Review 6.  Glycyl radical activating enzymes: structure, mechanism, and substrate interactions.

Authors:  Krista A Shisler; Joan B Broderick
Journal:  Arch Biochem Biophys       Date:  2014-01-31       Impact factor: 4.013

7.  Implications of the inability of Listeria monocytogenes EGD-e to grow anaerobically due to a deletion in the class III NrdD ribonucleotide reductase for its use as a model laboratory strain.

Authors:  Amos Ofer; Jürgen Kreft; Derek T Logan; Gerald Cohen; Ilya Borovok; Yair Aharonowitz
Journal:  J Bacteriol       Date:  2011-04-08       Impact factor: 3.490

8.  Generation of the glycyl radical of the anaerobic Escherichia coli ribonucleotide reductase requires a specific activating enzyme.

Authors:  X Sun; R Eliasson; E Pontis; J Andersson; G Buist; B M Sjöberg; P Reichard
Journal:  J Biol Chem       Date:  1995-02-10       Impact factor: 5.157

9.  In vitro characterization of AtsB, a radical SAM formylglycine-generating enzyme that contains three [4Fe-4S] clusters.

Authors:  Tyler L Grove; Kyung-Hoon Lee; Jennifer St Clair; Carsten Krebs; Squire J Booker
Journal:  Biochemistry       Date:  2008-06-18       Impact factor: 3.162

10.  Subunit structure of benzylsuccinate synthase.

Authors:  Lei Li; Dustin P Patterson; Christel C Fox; Brian Lin; Peter W Coschigano; E Neil G Marsh
Journal:  Biochemistry       Date:  2009-02-17       Impact factor: 3.162
View more
  10 in total

Review 1.  Bacterial microcompartments.

Authors:  Cheryl A Kerfeld; Clement Aussignargues; Jan Zarzycki; Fei Cai; Markus Sutter
Journal:  Nat Rev Microbiol       Date:  2018-03-05       Impact factor: 60.633

2.  A prominent glycyl radical enzyme in human gut microbiomes metabolizes trans-4-hydroxy-l-proline.

Authors:  B J Levin; Y Y Huang; S C Peck; Y Wei; A Martínez-Del Campo; J A Marks; E A Franzosa; C Huttenhower; E P Balskus
Journal:  Science       Date:  2017-02-10       Impact factor: 47.728

Review 3.  Advances in the World of Bacterial Microcompartments.

Authors:  Andrew M Stewart; Katie L Stewart; Todd O Yeates; Thomas A Bobik
Journal:  Trends Biochem Sci       Date:  2021-01-11       Impact factor: 13.807

4.  In Vitro Characterization and Concerted Function of Three Core Enzymes of a Glycyl Radical Enzyme - Associated Bacterial Microcompartment.

Authors:  Jan Zarzycki; Markus Sutter; Niña Socorro Cortina; Tobias J Erb; Cheryl A Kerfeld
Journal:  Sci Rep       Date:  2017-02-16       Impact factor: 4.379

5.  A glycyl radical enzyme enables hydrogen sulfide production by the human intestinal bacterium Bilophila wadsworthia.

Authors:  Spencer C Peck; Karin Denger; Anna Burrichter; Stephania M Irwin; Emily P Balskus; David Schleheck
Journal:  Proc Natl Acad Sci U S A       Date:  2019-02-04       Impact factor: 11.205

Review 6.  Glycyl Radical Enzyme-Associated Microcompartments: Redox-Replete Bacterial Organelles.

Authors:  Bryan Ferlez; Markus Sutter; Cheryl A Kerfeld
Journal:  mBio       Date:  2019-01-08       Impact factor: 7.867

7.  Molecular basis of C-S bond cleavage in the glycyl radical enzyme isethionate sulfite-lyase.

Authors:  Christopher D Dawson; Stephania M Irwin; Lindsey R F Backman; Chip Le; Jennifer X Wang; Vyshnavi Vennelakanti; Zhongyue Yang; Heather J Kulik; Catherine L Drennan; Emily P Balskus
Journal:  Cell Chem Biol       Date:  2021-03-26       Impact factor: 8.116

8.  Genomic reconstruction of short-chain fatty acid production by the human gut microbiota.

Authors:  Maria S Frolova; Inna A Suvorova; Stanislav N Iablokov; Sergei N Petrov; Dmitry A Rodionov
Journal:  Front Mol Biosci       Date:  2022-08-11

9.  Molecular basis for catabolism of the abundant metabolite trans-4-hydroxy-L-proline by a microbial glycyl radical enzyme.

Authors:  Lindsey Rf Backman; Yolanda Y Huang; Mary C Andorfer; Brian Gold; Ronald T Raines; Emily P Balskus; Catherine L Drennan
Journal:  Elife       Date:  2020-03-17       Impact factor: 8.140

Review 10.  New tricks for the glycyl radical enzyme family.

Authors:  Lindsey R F Backman; Michael A Funk; Christopher D Dawson; Catherine L Drennan
Journal:  Crit Rev Biochem Mol Biol       Date:  2017-09-13       Impact factor: 8.250
  10 in total

北京卡尤迪生物科技股份有限公司 © 2022-2023.