Literature DB >> 27213685

Replacement of Thymidine by a Modified Base in the Escherichia coli Genome.

Angad P Mehta1, Han Li1, Sean A Reed1, Lubica Supekova1, Tsotne Javahishvili1, Peter G Schultz1.   

Abstract

Prokaryotic and eukaryotic genomic DNA is comprised of the four building blocks A, G, C, and T. We have begun to explore the consequences of replacing a large fraction or all of a nucleoside in genomic DNA with a modified nucleoside. As a first step we have investigated the possibility of replacement of T by 2'-deoxy-5-(hydroxymethyl)uridine (5hmU) in the genomic DNA of Escherichia coli. Metabolic engineering with phage genes followed by random mutagenesis enabled us to achieve approximately 75% replacement of T by 5hmU in the E. coli genome and in plasmids.

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Year:  2016        PMID： 27213685      PMCID： PMC5218517          DOI： 10.1021/jacs.6b03904

Source DB:  PubMed          Journal:  J Am Chem Soc        ISSN： 0002-7863            Impact factor:   15.419


  26 in total

1.  An unnatural base pair for incorporating amino acid analogs into proteins.

Authors:  Ichiro Hirao; Takashi Ohtsuki; Tsuyoshi Fujiwara; Tsuneo Mitsui; Tomoko Yokogawa; Taeko Okuni; Hiroshi Nakayama; Koji Takio; Takashi Yabuki; Takanori Kigawa; Koichiro Kodama; Takashi Yokogawa; Kazuya Nishikawa; Shigeyuki Yokoyama
Journal:  Nat Biotechnol       Date:  2002-02       Impact factor: 54.908

Review 2.  Beyond A, C, G and T: augmenting nature's alphabet.

Authors:  Allison A Henry; Floyd E Romesberg
Journal:  Curr Opin Chem Biol       Date:  2003-12       Impact factor: 8.822

3.  Difluorotoluene, a Nonpolar Isostere for Thymine, Codes Specifically and Efficiently for Adenine in DNA Replication.

Authors:  Sean Moran; Rex X-F Ren; Squire Rumney; Eric T Kool
Journal:  J Am Chem Soc       Date:  1997-02-26       Impact factor: 15.419

4.  Ribosome-mediated incorporation of a non-standard amino acid into a peptide through expansion of the genetic code.

Authors:  J D Bain; C Switzer; A R Chamberlin; S A Benner
Journal:  Nature       Date:  1992-04-09       Impact factor: 49.962

Review 5.  Adenylates: bound and unbound.

Authors:  N J Leonard
Journal:  Biopolymers       Date:  1985-01       Impact factor: 2.505

6.  Synthesis and biochemical evaluation of 2'-deoxy-lin-benzoadenosine phosphates.

Authors:  R A Lessor; K J Gibson; N J Leonard
Journal:  Biochemistry       Date:  1984-08-14       Impact factor: 3.162

7.  Structure of a copper-mediated base pair in DNA.

Authors:  S Atwell; E Meggers; G Spraggon; P G Schultz
Journal:  J Am Chem Soc       Date:  2001-12-12       Impact factor: 15.419

8.  A thymidine triphosphate shape analog lacking Watson-Crick pairing ability is replicated with high sequence selectivity.

Authors:  S Moran; R X Ren; E T Kool
Journal:  Proc Natl Acad Sci U S A       Date:  1997-09-30       Impact factor: 11.205

9.  Synthesis of 6-(2-thienyl)purine nucleoside derivatives that form unnatural base pairs with pyridin-2-one nucleosides.

Authors:  T Fujiwara; M Kimoto; H Sugiyama; I Hirao; S Yokoyama
Journal:  Bioorg Med Chem Lett       Date:  2001-08-20       Impact factor: 2.823

10.  Biochemical characterization of recombinant β-glucosyltransferase and analysis of global 5-hydroxymethylcytosine in unique genomes.

Authors:  Jolyon Terragni; Jurate Bitinaite; Yu Zheng; Sriharsa Pradhan
Journal:  Biochemistry       Date:  2012-01-27       Impact factor: 3.162
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  9 in total

1.  Replacement of 2'-Deoxycytidine by 2'-Deoxycytidine Analogues in the E. coli Genome.

Authors:  Angad P Mehta; Han Li; Sean A Reed; Lubica Supekova; Tsotne Javahishvili; Peter G Schultz
Journal:  J Am Chem Soc       Date:  2016-10-20       Impact factor: 15.419

Review 2.  Rebooting life: engineering non-natural nucleic acids, proteins and metabolites in microorganisms.

Authors:  Shriya Hans; Nilesh Kumar; Nisarg Gohil; Khushal Khambhati; Gargi Bhattacharjee; Shalini S Deb; Rupesh Maurya; Vinod Kumar; Shamlan M S Reshamwala; Vijai Singh
Journal:  Microb Cell Fact       Date:  2022-05-28       Impact factor: 6.352

3.  A Tool for the Import of Natural and Unnatural Nucleoside Triphosphates into Bacteria.

Authors:  Aaron W Feldman; Emil C Fischer; Michael P Ledbetter; Jen-Yu Liao; John C Chaput; Floyd E Romesberg
Journal:  J Am Chem Soc       Date:  2018-01-17       Impact factor: 15.419

4.  Modified nucleoside triphosphates in bacterial research for in vitro and live-cell applications.

Authors:  Adeline Espinasse; Hannah K Lembke; Angela A Cao; Erin E Carlson
Journal:  RSC Chem Biol       Date:  2020-09-14

5.  Characterization of a triad of genes in cyanophage S-2L sufficient to replace adenine by 2-aminoadenine in bacterial DNA.

Authors:  Dariusz Czernecki; Frédéric Bonhomme; Pierre-Alexandre Kaminski; Marc Delarue
Journal:  Nat Commun       Date:  2021-08-05       Impact factor: 14.919

6.  Discovery of an Unnatural DNA Modification Derived from a Natural Secondary Metabolite.

Authors:  Tong Wang; Rahul M Kohli
Journal:  Cell Chem Biol       Date:  2020-10-13       Impact factor: 8.116

7.  In vitro Type II Restriction of Bacteriophage DNA With Modified Pyrimidines.

Authors:  Kiersten Flodman; Ivan R Corrêa; Nan Dai; Peter Weigele; Shuang-Yong Xu
Journal:  Front Microbiol       Date:  2020-10-27       Impact factor: 5.640

Review 8.  Modified nucleic acids: replication, evolution, and next-generation therapeutics.

Authors:  Karen Duffy; Sebastian Arangundy-Franklin; Philipp Holliger
Journal:  BMC Biol       Date:  2020-09-02       Impact factor: 7.431

Review 9.  Synthetic Life with Alternative Nucleic Acids as Genetic Materials.

Authors:  Peng Nie; Yanfen Bai; Hui Mei
Journal:  Molecules       Date:  2020-07-31       Impact factor: 4.411
  9 in total

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