Literature DB >> 27163

Stabilization of human fibroblast interferon purified on concanavalin A-agarose.

J W Heine, A J Mikulski, E Sulkowski, W A Carter.   

Abstract

Human fibroblast interferon, obtained by chromatography on concanavalin A-agarose, was stable for at least a month in 30--50 per cent ethylene glycol at 4 degrees, --20 degrees, and --70 degrees C. The succinct point of the present finding is that human fibroblast interferon may be stabilized by ethylene glycol alone without the addition of bovine serum albumin and 'back-contamination' of the interferon preparation.

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Year:  1978        PMID: 27163     DOI: 10.1007/BF01315680

Source DB:  PubMed          Journal:  Arch Virol        ISSN: 0304-8608            Impact factor:   2.574


  12 in total

1.  Effect of chaotropic salts and protein denaturants on the thermal stability of mouse fibroblast interferon.

Authors:  R J Jariwalla; S E Grossberg; J J Sedmak
Journal:  J Gen Virol       Date:  1977-04       Impact factor: 3.891

2.  Purification of interferon by adsorption chromatography on controlled pore glass.

Authors:  V G Edy; I A Braude; E De Clercq; A Billiau; P De Somer
Journal:  J Gen Virol       Date:  1976-12       Impact factor: 3.891

3.  Interferon: purification and initial characterization from human diploid cells.

Authors:  E Knight
Journal:  Proc Natl Acad Sci U S A       Date:  1976-02       Impact factor: 11.205

4.  Stabilisation of mouse and human interferons by acid pH against inactivation due to shaking and guanidine hydrochloride.

Authors:  V G Edy; A Billiau; M Joniau; P De Somer
Journal:  Proc Soc Exp Biol Med       Date:  1974-05

5.  Production of high-titered interferon in cultures of human diploid cells.

Authors:  E A Havell; J Vilcek
Journal:  Antimicrob Agents Chemother       Date:  1972-12       Impact factor: 5.191

6.  Structural requirements of the rI n -rC n complex for induction of human interferon.

Authors:  W A Carter; P M Pitha; L W Marshall; I Tazawa; S Tazawa; P O Ts'o
Journal:  J Mol Biol       Date:  1972-10-14       Impact factor: 5.469

7.  Inactivation of interferon: the effect of protonation.

Authors:  L W Marshall; P M Pitha; W A Carter
Journal:  Virology       Date:  1972-05       Impact factor: 3.616

8.  Binding of human fibroblast interferon to concanavalin A-agarose. Involvement of carbohydrate recognition and hydrophobic interaction.

Authors:  M W Davey; E Sulkowski; W A Carter
Journal:  Biochemistry       Date:  1976-02-10       Impact factor: 3.162

9.  Hydrophobic interaction of human, mouse, and rabbit interferons with immobilized hydrocarbons.

Authors:  M W Davey; E Sulkowski; W A Carter
Journal:  J Biol Chem       Date:  1976-12-10       Impact factor: 5.157

10.  Partial purification of human interferon by affinity chromatography.

Authors:  C B Anfinsen; S Bose; L Corley; D Gurari-Rotman
Journal:  Proc Natl Acad Sci U S A       Date:  1974-08       Impact factor: 11.205

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