Literature DB >> 1036458

Purification of interferon by adsorption chromatography on controlled pore glass.

V G Edy, I A Braude, E De Clercq, A Billiau, P De Somer.   

Abstract

Human fibroblast and mouse L929 cell interferons can be purified by adsoprtion to and subsequent elution from Controlled Pore Glass. Purification of 40 to 90-fold to specific activities of 1 to 5 times 10(6) units/mg of protein can be achieved in a single step, with good recovery of activity. Human leukocyte interferon does not bind to the glass and cannot be purified in this way.

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Year:  1976        PMID: 1036458     DOI: 10.1099/0022-1317-33-3-517

Source DB:  PubMed          Journal:  J Gen Virol        ISSN: 0022-1317            Impact factor:   3.891


  7 in total

1.  Elimination of size and charge heterogeneities of human leukocyte interferons by chemical cleavage.

Authors:  W E Stewart; L S Lin; M Wiranowska-Stewart; K Cantell
Journal:  Proc Natl Acad Sci U S A       Date:  1977-10       Impact factor: 11.205

2.  Human interferon: mass production in a newly established cell line, MG-63.

Authors:  A Billiau; V G Edy; H Heremans; J Van Damme; J Desmyter; J A Georgiades; P De Somer
Journal:  Antimicrob Agents Chemother       Date:  1977-07       Impact factor: 5.191

3.  Stabilization of human fibroblast interferon purified on concanavalin A-agarose.

Authors:  J W Heine; A J Mikulski; E Sulkowski; W A Carter
Journal:  Arch Virol       Date:  1978       Impact factor: 2.574

Review 4.  Differences between human alpha (leukocyte) and beta (fibroblast) interferons.

Authors:  T G Hayes
Journal:  Arch Virol       Date:  1981       Impact factor: 2.574

5.  Rescue of SV40 from interferon-treated heterokaryons.

Authors:  L W Mozes
Journal:  Arch Virol       Date:  1982       Impact factor: 2.574

6.  Human fibroblast interferon for clinical trials: production, partial purification, and characterization.

Authors:  A Billiau; J V Damme; F V Leuven; V G Edy; M De Ley; J J Cassiman; H Van de Berghe; P De Somer
Journal:  Antimicrob Agents Chemother       Date:  1979-07       Impact factor: 5.191

7.  Human fibroblast interferon for clinical trials: pharmacokinetics and tolerability in experimental animals and humans.

Authors:  A Billiau; P De Somers; V G Edy; E De Clercq; H Heremans
Journal:  Antimicrob Agents Chemother       Date:  1979-07       Impact factor: 5.191

  7 in total

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