Literature DB >> 6088071

Human transforming growth factor-alpha: precursor structure and expression in E. coli.

R Derynck, A B Roberts, M E Winkler, E Y Chen, D V Goeddel.   

Abstract

Transforming growth factor-alpha (TGF-alpha) is secreted by many human tumors and can induce the reversible transformation of nontransformed cell lines. Using long synthetic deoxyoligonucleotides as hybridization probes we isolated an exon coding for a portion of TGF-alpha from a human genomic DNA library. Utilizing this exon as a probe, a cell line derived from a human renal cell carcinoma was identified as a source of TGF-alpha mRNA. A cloned TGF-alpha cDNA was isolated from a cDNA library prepared using RNA from this cell line, and was found to encode a precursor polypeptide of 160 amino acids. The 50 amino acid mature TGF-alpha produced by expression of the appropriate coding sequence in E. coli binds to the epidermal growth factor receptor and induces the anchorage independence of normal mammalian cells in culture.

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Year:  1984        PMID： 6088071     DOI： 10.1016/0092-8674(84)90550-6

Source DB:  PubMed          Journal:  Cell        ISSN： 0092-8674            Impact factor:   41.582


  180 in total

1.  Neuregulin-3 (NRG3): a novel neural tissue-enriched protein that binds and activates ErbB4.

Authors:  D Zhang; M X Sliwkowski; M Mark; G Frantz; R Akita; Y Sun; K Hillan; C Crowley; J Brush; P J Godowski
Journal:  Proc Natl Acad Sci U S A       Date:  1997-09-02       Impact factor: 11.205

Review 2.  Monoclonal antibodies to epidermal growth factor receptors in studies of receptor structure and function.

Authors:  T Kawamoto; G H Sato; K Takahashi; M Nishi; S Taniguchi; J D Sato
Journal:  Cytotechnology       Date:  1990-05       Impact factor: 2.058

Review 3.  The extracellular regulation of growth factor action.

Authors:  R Flaumenhaft; D B Rifkin
Journal:  Mol Biol Cell       Date:  1992-10       Impact factor: 4.138

4.  Expression of transforming growth factor alpha in human tissues: immunohistochemical study and northern blot analysis.

Authors:  W Yasui; Z Q Ji; H Kuniyasu; A Ayhan; H Yokozaki; H Ito; E Tahara
Journal:  Virchows Arch A Pathol Anat Histopathol       Date:  1992

5.  A novel endothelial cell surface receptor tyrosine kinase with extracellular epidermal growth factor homology domains.

Authors:  J Partanen; E Armstrong; T P Mäkelä; J Korhonen; M Sandberg; R Renkonen; S Knuutila; K Huebner; K Alitalo
Journal:  Mol Cell Biol       Date:  1992-04       Impact factor: 4.272

6.  cDNA cloning of a mouse mammary epithelial cell surface protein reveals the existence of epidermal growth factor-like domains linked to factor VIII-like sequences.

Authors:  J D Stubbs; C Lekutis; K L Singer; A Bui; D Yuzuki; U Srinivasan; G Parry
Journal:  Proc Natl Acad Sci U S A       Date:  1990-11       Impact factor: 11.205

7.  Differential expression of transforming growth factors alpha and beta in rat intestinal epithelial cells.

Authors:  S Y Koyama; D K Podolsky
Journal:  J Clin Invest       Date:  1989-05       Impact factor: 14.808

8.  Tumor necrosis factor alpha-converting enzyme mediates MUC5AC mucin expression in cultured human airway epithelial cells.

Authors:  Matt X G Shao; Iris F Ueki; Jay A Nadel
Journal:  Proc Natl Acad Sci U S A       Date:  2003-09-12       Impact factor: 11.205

9.  Structural analysis of the uEGF gene in the sea urchin strongylocentrotus purpuratus reveals more similarity to vertebrate than to invertebrate genes with EGF-like repeats.

Authors:  M G Delgadillo-Reynoso; D R Rollo; D A Hursh; R A Raff
Journal:  J Mol Evol       Date:  1989-10       Impact factor: 2.395

10.  Altered EGFR localization and degradation in human breast cancer cells with an amphiregulin/EGFR autocrine loop.

Authors:  Nicole E Willmarth; Andrea Baillo; Michele L Dziubinski; Kristy Wilson; David J Riese; Stephen P Ethier
Journal:  Cell Signal       Date:  2008-10-14       Impact factor: 4.315
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