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Literature DB >> 27088314

siFLIM: single-image frequency-domain FLIM provides fast and photon-efficient lifetime data.

Marcel Raspe¹, Katarzyna M Kedziora¹, Bram van den Broek¹, Qiaole Zhao², Sander de Jong³, Johan Herz³, Marieke Mastop⁴, Joachim Goedhart^4,5, Theodorus W J Gadella^4,5, Ian T Young², Kees Jalink^1,4,5.

Abstract

We developed single-image fluorescence lifetime imaging microscopy (siFLIM), a method for acquiring quantitative lifetime images from a single exposure. siFLIM takes advantage of a new generation of dedicated cameras that simultaneously record two 180°-phase-shifted images, and it allows for video-rate lifetime imaging with minimal phototoxicity and bleaching. siFLIM is also inherently immune to artifacts stemming from rapid cellular movements and signal transients.

Mesh：

Substances：

Year: 2016 PMID： 27088314 DOI： 10.1038/nmeth.3836

Source DB: PubMed Journal: Nat Methods ISSN： 1548-7091 Impact factor: 28.547

22 in total

1. Measurement of nanosecond time-resolved fluorescence with a directly gated interline CCD camera.

Authors: A C Mitchell; J E Wall; J G Murray; C G Morgan
Journal: J Microsc Date: 2002-06 Impact factor: 1.758

2. The phasor approach to fluorescence lifetime imaging analysis.

Authors: Michelle A Digman; Valeria R Caiolfa; Moreno Zamai; Enrico Gratton
Journal: Biophys J Date: 2007-11-02 Impact factor: 4.033

3. HL-1 cells: a cardiac muscle cell line that contracts and retains phenotypic characteristics of the adult cardiomyocyte.

Authors: W C Claycomb; N A Lanson; B S Stallworth; D B Egeland; J B Delcarpio; A Bahinski; N J Izzo
Journal: Proc Natl Acad Sci U S A Date: 1998-03-17 Impact factor: 11.205

4. Modulated electron-multiplied fluorescence lifetime imaging microscope: all-solid-state camera for fluorescence lifetime imaging.

Authors: Qiaole Zhao; Ben Schelen; Raymond Schouten; Rein van den Oever; René Leenen; Harry van Kuijk; Inge Peters; Frank Polderdijk; Jan Bosiers; Marcel Raspe; Kees Jalink; Jan Geert Sander de Jong; Bert van Geest; Karel Stoop; Ian Ted Young
Journal: J Biomed Opt Date: 2012-12 Impact factor: 3.170

5. A practical implementation of multifrequency widefield frequency-domain fluorescence lifetime imaging microscopy.

Authors: Hongtao Chen; Enrico Gratton
Journal: Microsc Res Tech Date: 2013-01-07 Impact factor: 2.769

6. Fluorescence lifetime-resolved pH imaging of living cells.

Authors: Hai-Jui Lin; Petr Herman; Joseph R Lakowicz
Journal: Cytometry A Date: 2003-04 Impact factor: 4.355

7. A dark yellow fluorescent protein (YFP)-based Resonance Energy-Accepting Chromoprotein (REACh) for Förster resonance energy transfer with GFP.

Authors: Sundar Ganesan; Simon M Ameer-Beg; Tony T C Ng; Borivoj Vojnovic; Fred S Wouters
Journal: Proc Natl Acad Sci U S A Date: 2006-03-06 Impact factor: 11.205

8. A mTurquoise-based cAMP sensor for both FLIM and ratiometric read-out has improved dynamic range.

Authors: Jeffrey B Klarenbeek; Joachim Goedhart; Mark A Hink; Theodorus W J Gadella; Kees Jalink
Journal: PLoS One Date: 2011-04-29 Impact factor: 3.240

9. A comparison of donor-acceptor pairs for genetically encoded FRET sensors: application to the Epac cAMP sensor as an example.

Authors: Gerard N M van der Krogt; Janneke Ogink; Bas Ponsioen; Kees Jalink
Journal: PLoS One Date: 2008-04-02 Impact factor: 3.240

10. Activation of endosomal dynein motors by stepwise assembly of Rab7-RILP-p150Glued, ORP1L, and the receptor betalll spectrin.

Authors: Marie Johansson; Nuno Rocha; Wilbert Zwart; Ingrid Jordens; Lennert Janssen; Coenraad Kuijl; Vesa M Olkkonen; Jacques Neefjes
Journal: J Cell Biol Date: 2007-02-05 Impact factor: 10.539

20 in total

siFLIM: single-image frequency-domain FLIM provides fast and photon-efficient lifetime data.

1. Measurement of nanosecond time-resolved fluorescence with a directly gated interline CCD camera.

2. The phasor approach to fluorescence lifetime imaging analysis.

3. HL-1 cells: a cardiac muscle cell line that contracts and retains phenotypic characteristics of the adult cardiomyocyte.

4. Modulated electron-multiplied fluorescence lifetime imaging microscope: all-solid-state camera for fluorescence lifetime imaging.

5. A practical implementation of multifrequency widefield frequency-domain fluorescence lifetime imaging microscopy.

6. Fluorescence lifetime-resolved pH imaging of living cells.

7. A dark yellow fluorescent protein (YFP)-based Resonance Energy-Accepting Chromoprotein (REACh) for Förster resonance energy transfer with GFP.

8. A mTurquoise-based cAMP sensor for both FLIM and ratiometric read-out has improved dynamic range.

9. A comparison of donor-acceptor pairs for genetically encoded FRET sensors: application to the Epac cAMP sensor as an example.

10. Activation of endosomal dynein motors by stepwise assembly of Rab7-RILP-p150Glued, ORP1L, and the receptor betalll spectrin.

Review 1. Quantifying lipid changes in various membrane compartments using lipid binding protein domains.

2. Nonparametric empirical Bayesian framework for fluorescence-lifetime imaging microscopy.

3. Characterization of a spectrally diverse set of fluorescent proteins as FRET acceptors for mTurquoise2.

4. Real-time pixelwise phasor analysis for video-rate two-photon fluorescence lifetime imaging microscopy.

5. Optical Estimation of Absolute Membrane Potential Using One- and Two-Photon Fluorescence Lifetime Imaging Microscopy.

6. Optical estimation of absolute membrane potential using fluorescence lifetime imaging.

Review 7. Measuring Absolute Membrane Potential Across Space and Time.

8. Luminescence lifetime imaging of three-dimensional biological objects.

Review 9. Exploring cells with targeted biosensors.

10. Fluorescence lifetime imaging microscopy: fundamentals and advances in instrumentation, analysis, and applications.