Nirmal Marasini1, Ashwini K Giddam1, Khairunnisa A Ghaffar1, Michael R Batzloff2, Michael F Good2, Mariusz Skwarczynski1, Istvan Toth1,3,4. 1. School of Chemistry & Molecular Biosciences, The University of Queensland, St Lucia, QLD 4072, Australia. 2. Institute for Glycomics, Griffith University, Gold Coast, QLD, Australia. 3. School of Pharmacy, The University of Queensland, Woolloongabba, QLD 4102, Australia. 4. Institute for Molecular Biosciences, The University of Queensland, St Lucia, QLD 4072, Australia.
Abstract
AIM: To develop an oral nanovaccine delivery system for lipopeptide-based vaccine candidate against group A Streptococcus. MATERIALS & METHODS: Lipid-core peptide-1-loaded nanoliposomes were prepared as a template and coated with opposite-charged polyelectrolytes to produce particles with size <200 nm. Efficacy of this oral nanovaccine delivery system was evaluated in mice model. RESULTS: Polymer-coated liposomes produced significantly higher antigen-specific mucosal IgA and systemic IgG titers in comparison to vaccine formulated with a strong mucosal adjuvant upon oral immunization in mice. Moreover, high levels of systemic antibody titers were retained even at day 185 postprimary immunization. CONCLUSION: Efficient oral delivery platform for lipopeptide-based vaccines has been developed.
AIM: To develop an oral nanovaccine delivery system for lipopeptide-based vaccine candidate against group A Streptococcus. MATERIALS & METHODS:Lipid-core peptide-1-loaded nanoliposomes were prepared as a template and coated with opposite-charged polyelectrolytes to produce particles with size <200 nm. Efficacy of this oral nanovaccine delivery system was evaluated in mice model. RESULTS: Polymer-coated liposomes produced significantly higher antigen-specific mucosal IgA and systemic IgG titers in comparison to vaccine formulated with a strong mucosal adjuvant upon oral immunization in mice. Moreover, high levels of systemic antibody titers were retained even at day 185 postprimary immunization. CONCLUSION: Efficient oral delivery platform for lipopeptide-based vaccines has been developed.
Entities:
Keywords:
group A Streptococcus; lipid-core peptide; liposomes; mucosal immunology; nanoparticles; oral delivery; peptides; vaccines