Allison J Pollock1, David B Allen2, Donald Wiebe2, Jens Eickhoff2, Michael MacDonald2, Mei Baker3. 1. University of Wisconsin School of Medicine and Public Health, 600 Highland Avenue, Madison, WI 53792, USA. Electronic address: apollock@pediatrics.wisc.edu. 2. University of Wisconsin School of Medicine and Public Health, 600 Highland Avenue, Madison, WI 53792, USA. 3. University of Wisconsin School of Medicine and Public Health, 600 Highland Avenue, Madison, WI 53792, USA; Wisconsin State Laboratory of Hygiene, 2601 Agriculture Dr, Madison, WI 53718, USA.
Abstract
PURPOSE: Gestational diabetes influences risk for future metabolic disease including type 2 diabetes. Hemoglobin A1c (HbA1c) measurement assesses hemoglobin A glycosylation, and could theoretically be used as a test to estimate gestational glucose exposure. HbA1c assay on dried blood spots (DBS) is needed before potential application to statewide newborn screening (NBS) population studies. The study aimed to establish a reliable method to measure HbA1c on NBS DBS specimens. De-identified blood was used to generate trials to evaluate stability of HbA1c in DBS, optimal elution time, and stability of eluted blood. RESULTS: Analysis of DBS stability HbA1c measurements from 3 to 6days after collection overestimated HbA1c values by a bias factor between 0.83 and 0.87. Sixty minutes of elution time produced maximal reproducibility and minimal bias of results. Within assay standard deviation: 0.058; average bias: -0.02%. Stability of eluted blood did not vary significantly between days 0-2 after DBS elution. CONCLUSIONS: Measurement of HbA1c levels on DBS from human blood is feasible. Results suggest new method using DBS to measure HbA1c level with the following characteristics: optimal time for sample analysis 3-6days after collection, elution time of 60min and eluted blood analysis within 2days of elution. Measurement of neonatal HbA1c could provide insight regarding the infant's in utero exposure to glucose.
PURPOSE:Gestational diabetes influences risk for future metabolic disease including type 2 diabetes. Hemoglobin A1c (HbA1c) measurement assesses hemoglobin A glycosylation, and could theoretically be used as a test to estimate gestational glucose exposure. HbA1c assay on dried blood spots (DBS) is needed before potential application to statewide newborn screening (NBS) population studies. The study aimed to establish a reliable method to measure HbA1c on NBSDBS specimens. De-identified blood was used to generate trials to evaluate stability of HbA1c in DBS, optimal elution time, and stability of eluted blood. RESULTS: Analysis of DBS stability HbA1c measurements from 3 to 6days after collection overestimated HbA1c values by a bias factor between 0.83 and 0.87. Sixty minutes of elution time produced maximal reproducibility and minimal bias of results. Within assay standard deviation: 0.058; average bias: -0.02%. Stability of eluted blood did not vary significantly between days 0-2 after DBS elution. CONCLUSIONS: Measurement of HbA1c levels on DBS from human blood is feasible. Results suggest new method using DBS to measure HbA1c level with the following characteristics: optimal time for sample analysis 3-6days after collection, elution time of 60min and eluted blood analysis within 2days of elution. Measurement of neonatal HbA1c could provide insight regarding the infant's in utero exposure to glucose.
Authors: M Koga; J Murai; H Saito; Y Yamada; T Mori; S Suno; K Takeuchi; S Suzuki; K Fujieda; S Kasayama Journal: J Perinatol Date: 2010-12-16 Impact factor: 2.521
Authors: Shin Y Kim; William Sappenfield; Andrea J Sharma; Hoyt G Wilson; Connie L Bish; Hamisu M Salihu; Lucinda J England Journal: Obesity (Silver Spring) Date: 2013-01 Impact factor: 5.002