| Literature DB >> 27013429 |
Nuno Rodrigues Faria1,2, Raimunda do Socorro da Silva Azevedo3, Oliver G Pybus2,4, Marcio R T Nunes1,5, Pedro F C Vasconcelos3, Moritz U G Kraemer2, Renato Souza6, Mariana Sequetin Cunha6, Sarah C Hill2, Julien Thézé2, Michael B Bonsall2, Thomas A Bowden7, Ilona Rissanen7, Iray Maria Rocco6, Juliana Silva Nogueira6, Adriana Yurika Maeda6, Fernanda Giseli da Silva Vasami6, Fernando Luiz de Lima Macedo6, Akemi Suzuki6, Sueli Guerreiro Rodrigues3, Ana Cecilia Ribeiro Cruz3, Bruno Tardeli Nunes3, Daniele Barbosa de Almeida Medeiros3, Daniela Sueli Guerreiro Rodrigues3, Alice Louize Nunes Queiroz3, Eliana Vieira Pinto da Silva3, Daniele Freitas Henriques3, Elisabeth Salbe Travassos da Rosa3, Consuelo Silva de Oliveira3, Livia Caricio Martins3, Helena Baldez Vasconcelos3, Livia Medeiros Neves Casseb3, Darlene de Brito Simith3, Jane P Messina2,4, Leandro Abade2, José Lourenço2, Luiz Carlos Junior Alcantara8, Maricélia Maia de Lima9, Marta Giovanetti8, Simon I Hay10,7, Rodrigo Santos de Oliveira1, Poliana da Silva Lemos1, Layanna Freitas de Oliveira1, Clayton Pereira Silva de Lima1, Sandro Patroca da Silva1, Janaina Mota de Vasconcelos1, Luciano Franco1, Jedson Ferreira Cardoso1, João Lídio da Silva Gonçalves Vianez-Júnior1, Daiana Mir11, Gonzalo Bello11, Edson Delatorre11, Kamran Khan12,13, Marisa Creatore14, Giovanini Evelim Coelho15, Wanderson Kleber de Oliveira15, Robert Tesh5.
Abstract
Brazil has experienced an unprecedented epidemic of Zika virus (ZIKV), with ~30,000 cases reported to date. ZIKV was first detected in Brazil in May 2015, and cases of microcephaly potentially associated with ZIKV infection were identified in November 2015. We performed next-generation sequencing to generate seven Brazilian ZIKV genomes sampled from four self-limited cases, one blood donor, one fatal adult case, and one newborn with microcephaly and congenital malformations. Results of phylogenetic and molecular clock analyses show a single introduction of ZIKV into the Americas, which we estimated to have occurred between May and December 2013, more than 12 months before the detection of ZIKV in Brazil. The estimated date of origin coincides with an increase in air passengers to Brazil from ZIKV-endemic areas, as well as with reported outbreaks in the Pacific Islands. ZIKV genomes from Brazil are phylogenetically interspersed with those from other South American and Caribbean countries. Mapping mutations onto existing structural models revealed the context of viral amino acid changes present in the outbreak lineage; however, no shared amino acid changes were found among the three currently available virus genomes from microcephaly cases. Municipality-level incidence data indicate that reports of suspected microcephaly in Brazil best correlate with ZIKV incidence around week 17 of pregnancy, although this correlation does not demonstrate causation. Our genetic description and analysis of ZIKV isolates in Brazil provide a baseline for future studies of the evolution and molecular epidemiology of this emerging virus in the Americas.Entities:
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Year: 2016 PMID： 27013429 PMCID： PMC4918795 DOI： 10.1126/science.aaf5036
Source DB: PubMed Journal: Science ISSN： 0036-8075 Impact factor: 47.728