Literature DB >> 269444

Stable association of the human transgenome and host murine chromosomes demonstrated with trispecific microcell hybrids.

R E Fournier, F H Ruddle.   

Abstract

Trispecific microcell hybrids were prepared by transferring limited numbers of chromosomes from a human/mouse gene-transfer cell line to a Chinese hamster recipient line. The donor cells employed were murine L-cells that stably expressed the human form of the enzyme hypoxanthine phosphoribosyltransferase. Karyotypic, zymographic, and back-selection tests of the resulting human/mouse/Chinese hamster microcell hybrids provided strong genetic evidence for a stable association of the human transgenome with host murine chromosomes in stable gene-transfer cell lines. This association, which may represent physical integration of the transgenome into the host cell genome, occurred at multiple chromosomal sites.

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Year:  1977        PMID： 269444      PMCID： PMC431791          DOI： 10.1073/pnas.74.9.3937

Source DB:  PubMed          Journal:  Proc Natl Acad Sci U S A        ISSN： 0027-8424            Impact factor:   11.205


  17 in total

1.  SELECTION OF HYBRIDS FROM MATINGS OF FIBROBLASTS IN VITRO AND THEIR PRESUMED RECOMBINANTS.

Authors:  J W LITTLEFIELD
Journal:  Science       Date:  1964-08-14       Impact factor: 47.728

2.  Genetic analysis by chromosome-mediated gene transfer.

Authors:  O W McBride; R S Athwal
Journal:  In Vitro       Date:  1976-11

3.  Transfer of codominant markers by isolated metaphase chromosomes in Chinese hamster ovary cells.

Authors:  D A Spandidos; L Siminovitch
Journal:  Proc Natl Acad Sci U S A       Date:  1977-08       Impact factor: 11.205

Review 4.  A review of enzyme polymorphism, linkage and electrophoretic conditions for mouse and somatic cell hybrids in starch gels.

Authors:  E A Nichols; F H Ruddle
Journal:  J Histochem Cytochem       Date:  1973-12       Impact factor: 2.479

5.  A new electrophoretic-autoradiographic method for the visual detection of phosphotransferases.

Authors:  J A Tischfield; H P Bernhard; F H Ruddle
Journal:  Anal Biochem       Date:  1973-06       Impact factor: 3.365

6.  A sequential staining technique for the chromosomal analysis of the interspecific mouse/hamster and mouse/human somatic cell hybrids.

Authors:  C A Kozak; J B Lawrence; F H Ruddle
Journal:  Exp Cell Res       Date:  1977-03-01       Impact factor: 3.905

7.  Incorporation of isolated chromosomes and induction of hypoxanthine phosphoribosyltransferase in Chinese hamster cells.

Authors:  G J Wullems; J van der Horst; D Bootsma
Journal:  Somatic Cell Genet       Date:  1975-04

8.  Cotransfer of two linked human genes into cultured mouse cells.

Authors:  K Willecke; R Lange; A Krüger; T Reber
Journal:  Proc Natl Acad Sci U S A       Date:  1976-04       Impact factor: 11.205

9.  Serial transfer of a human gene to rodent cells by sequential chromosome-mediated gene transfer.

Authors:  R S Athwal; O W McBride
Journal:  Proc Natl Acad Sci U S A       Date:  1977-07       Impact factor: 11.205

10.  Microcell-mediated transfer of murine chromosomes into mouse, Chinese hamster, and human somatic cells.

Authors:  R E Fournier; F H Ruddle
Journal:  Proc Natl Acad Sci U S A       Date:  1977-01       Impact factor: 11.205
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  22 in total

1.  Molecular analysis of chromosome-mediated gene transfer.

Authors:  G A Scangos; K M Huttner; S Silverstein; F H Ruddle
Journal:  Proc Natl Acad Sci U S A       Date:  1979-08       Impact factor: 11.205

2.  Results and prospects of chromosomal gene transfer between cultured mammalian cells.

Authors:  K Willecke
Journal:  Theor Appl Genet       Date:  1978-05       Impact factor: 5.699

3.  Cotransfer of thymidine kinase and galactokinase genes by chromosome-mediated gene transfer.

Authors:  O W McBride; J W Burch; F H Ruddle
Journal:  Proc Natl Acad Sci U S A       Date:  1978-02       Impact factor: 11.205

Review 4.  The role of somatic cell genetics in human gene mapping.

Authors:  K H Grzeschik
Journal:  Experientia       Date:  1986-10-15

5.  Assignment of the gene for glyoxylase I to mouse chromosome 17 by somatic cell genetics.

Authors:  L Leinwand; E Nichols; F H Ruddle
Journal:  Biochem Genet       Date:  1978-08       Impact factor: 1.890

6.  Measurement and purification of human chromosomes by flow cytometry and sorting.

Authors:  A V Carrano; J W Gray; R G Langlois; K J Burkhart-Schultz; M A Van Dilla
Journal:  Proc Natl Acad Sci U S A       Date:  1979-03       Impact factor: 11.205

7.  c-myc Gene rearrangements involving gamma immunoglobulin heavy chain gene switch regions in murine plasmacytomas.

Authors:  L J Harris; E F Remmers; P Brodeur; R Riblet; P D'Eustachio; K B Marcu
Journal:  Nucleic Acids Res       Date:  1983-12-10       Impact factor: 16.971

8.  Construction of microcell hybrid clones containing specific mouse chromosomes: application to autosomes 8 and 17.

Authors:  R E Fournier; J A Frelinger
Journal:  Mol Cell Biol       Date:  1982-05       Impact factor: 4.272

9.  DNA sequence associated with chromosome translocations in mouse plasmacytomas.

Authors:  L J Harris; P D'Eustachio; F H Ruddle; K B Marcu
Journal:  Proc Natl Acad Sci U S A       Date:  1982-11       Impact factor: 11.205

10.  Assignment of the gene governing cellular ouabain resistance to Mus musculus chromosome 3 using human/mouse microcell hybrids.

Authors:  C A Kozak; R E Fournier; L A Leinwand; F H Ruddle
Journal:  Biochem Genet       Date:  1979-02       Impact factor: 1.890
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