F Almeida1, M I C Medeiros2, J D Kich3, J P Falcão1. 1. Faculdade de Ciências Farmacêuticas de Ribeirão Preto, Departamento de Análises Clínicas, Toxicológicas e Bromatológicas, Universidade de São Paulo (USP), Ribeirão Preto, SP, Brazil. 2. Instituto Adolfo Lutz de Ribeirão Preto, Ribeirão Preto, SP, Brazil. 3. Embrapa Suínos e Aves, Concórdia, SC, Brazil.
Abstract
AIMS: The aims of this study were to assess the pathogenic potential, antimicrobial resistance and genotypic diversity of Salmonella Typhimurium strains isolated in Brazil from swine (22) and the surrounding swine environment (5) from 2000 to 2012 and compare them to the profiles of 43 human strains isolated from 1983 to 2010, which had been previously studied. METHODS AND RESULTS: The presence of 12 SPI-1, SPI-2 and plasmid genes was assessed by PCR, the antimicrobial susceptibility to 13 antimicrobials was determined by the disc diffusion assay and genotyping was performed using pulsed-field gel electrophoresis (PFGE), multiple-locus variable-number of tandem repeats analysis (MLVA) and ERIC-PCR. More than 77·8% of the swine strains carried 10 or more of the virulence markers. Ten (37%) strains isolated from swine were multi-drug resistant (MDR). All the molecular typing techniques grouped the strains in two main clusters. Some strains isolated from swine and humans were allocated together in the PFGE-B2, MLVA-A1, MLVA-B and ERIC-A1 clusters. CONCLUSIONS: The genotyping results suggest that some strains isolated from swine and humans may descend from a common subtype and may indicate a possible risk of MDR S. Typhimurium with high frequency of virulence genes isolated from swine to contaminate humans in Brazil. SIGNIFICANCE AND IMPACT OF THE STUDY: This study provided new information about the pathogenic potential, antimicrobial resistance and genotypic diversity of S. Typhimurium isolates from swine origin in Brazil, the fourth largest producer of pigs worldwide.
AIMS: The aims of this study were to assess the pathogenic potential, antimicrobial resistance and genotypic diversity of Salmonella Typhimurium strains isolated in Brazil from swine (22) and the surrounding swine environment (5) from 2000 to 2012 and compare them to the profiles of 43 human strains isolated from 1983 to 2010, which had been previously studied. METHODS AND RESULTS: The presence of 12 SPI-1, SPI-2 and plasmid genes was assessed by PCR, the antimicrobial susceptibility to 13 antimicrobials was determined by the disc diffusion assay and genotyping was performed using pulsed-field gel electrophoresis (PFGE), multiple-locus variable-number of tandem repeats analysis (MLVA) and ERIC-PCR. More than 77·8% of the swine strains carried 10 or more of the virulence markers. Ten (37%) strains isolated from swine were multi-drug resistant (MDR). All the molecular typing techniques grouped the strains in two main clusters. Some strains isolated from swine and humans were allocated together in the PFGE-B2, MLVA-A1, MLVA-B and ERIC-A1 clusters. CONCLUSIONS: The genotyping results suggest that some strains isolated from swine and humans may descend from a common subtype and may indicate a possible risk of MDR S. Typhimurium with high frequency of virulence genes isolated from swine to contaminate humans in Brazil. SIGNIFICANCE AND IMPACT OF THE STUDY: This study provided new information about the pathogenic potential, antimicrobial resistance and genotypic diversity of S. Typhimurium isolates from swine origin in Brazil, the fourth largest producer of pigs worldwide.
Authors: Amanda Ap Seribelli; Patrick da Silva; Marcelo Ferreira da Cruz; Fernanda de Almeida; Miliane R Frazão; Marta I C Medeiros; Dália Dos P Rodrigues; Jalusa D Kich; Leandro de Jesus Benevides; Siomar de C Soares; Marc W Allard; Juliana Pfrimer Falcão Journal: Gut Pathog Date: 2021-04-28 Impact factor: 4.181
Authors: Elena Barilli; Cristina Bacci; Zulena StellaVilla; Giuseppe Merialdi; Mario D'Incau; Franco Brindani; Alice Vismarra Journal: Ital J Food Saf Date: 2018-07-03
Authors: Fernanda Almeida; Amanda Aparecida Seribelli; Marta Inês Cazentini Medeiros; Dália Dos Prazeres Rodrigues; Alessandro de MelloVarani; Yan Luo; Marc W Allard; Juliana Pfrimer Falcão Journal: PLoS One Date: 2018-08-13 Impact factor: 3.240
Authors: Renata F Rabello; Raquel R Bonelli; Bruno A Penna; Julia P Albuquerque; Rossiane M Souza; Aloysio M F Cerqueira Journal: Animals (Basel) Date: 2020-03-26 Impact factor: 2.752