Literature DB >> 26893376

The Arf GTPase-activating Protein, ASAP1, Binds Nonmuscle Myosin 2A to Control Remodeling of the Actomyosin Network.

Pei-Wen Chen1, Xiaoying Jian1, Sarah M Heissler2, Kang Le3, Ruibai Luo1, Lisa M Jenkins4, Attila Nagy2, Joel Moss3, James R Sellers2, Paul A Randazzo5.   

Abstract

ASAP1 regulates F-actin-based structures and functions, including focal adhesions (FAs) and circular dorsal ruffles (CDRs), cell spreading and migration. ASAP1 function requires its N-terminal BAR domain. We discovered that nonmuscle myosin 2A (NM2A) directly bound the BAR-PH tandem of ASAP1in vitro ASAP1 and NM2A co-immunoprecipitated and colocalized in cells. Knockdown of ASAP1 reduced colocalization of NM2A and F-actin in cells. Knockdown of ASAP1 or NM2A recapitulated each other's effects on FAs, cell migration, cell spreading, and CDRs. The NM2A-interacting BAR domain contributed to ASAP1 control of cell spreading and CDRs. Exogenous expression of NM2A rescued the effect of ASAP1 knockdown on CDRs but ASAP1 did not rescue NM2A knockdown defect in CDRs. Our results support the hypothesis that ASAP1 is a positive regulator of NM2A. Given other binding partners of ASAP1, ASAP1 may directly link signaling and the mechanical machinery of cell migration.
© 2016 by The American Society for Biochemistry and Molecular Biology, Inc.

Entities:  

Keywords:  ADP ribosylation factor (ARF); GTPase-activating protein (GAP); actin; cell biology; cell migration; cytoskeleton; myosin

Mesh：

Substances：

Year:  2016        PMID： 26893376      PMCID： PMC4817181          DOI： 10.1074/jbc.M115.701292

Source DB:  PubMed          Journal:  J Biol Chem        ISSN： 0021-9258            Impact factor:   5.157


  32 in total

1.  CrkL directs ASAP1 to peripheral focal adhesions.

Authors:  Atsushi Oda; Ikuo Wada; Koichi Miura; Katsuya Okawa; Toshihiko Kadoya; Takashi Kato; Hiroshi Nishihara; Masae Maeda; Shinya Tanaka; Kazuo Nagashima; Chiaki Nishitani; Kazuhiko Matsuno; Masaho Ishino; Laura M Machesky; Hiroyoshi Fujita; Paul Randazzo
Journal:  J Biol Chem       Date:  2003-01-08       Impact factor: 5.157

2.  Purification and characterization of platelet myosin.

Authors:  J L Daniel; J R Sellers
Journal:  Methods Enzymol       Date:  1992       Impact factor: 1.600

Review 3.  Foot and mouth: podosomes, invadopodia and circular dorsal ruffles.

Authors:  Roberto Buccione; James D Orth; Mark A McNiven
Journal:  Nat Rev Mol Cell Biol       Date:  2004-08       Impact factor: 94.444

4.  An alpha4 integrin-paxillin-Arf-GAP complex restricts Rac activation to the leading edge of migrating cells.

Authors:  Naoyuki Nishiya; William B Kiosses; Jaewon Han; Mark H Ginsberg
Journal:  Nat Cell Biol       Date:  2005-03-27       Impact factor: 28.824

5.  Expression of AMAP1, an ArfGAP, provides novel targets to inhibit breast cancer invasive activities.

Authors:  Yasuhito Onodera; Shigeru Hashimoto; Ari Hashimoto; Masaki Morishige; Yuichi Mazaki; Atsuko Yamada; Eiji Ogawa; Masashi Adachi; Takaki Sakurai; Toshiaki Manabe; Hiromi Wada; Nariaki Matsuura; Hisataka Sabe
Journal:  EMBO J       Date:  2005-02-17       Impact factor: 11.598

6.  Phosphoinositide-dependent activation of the ADP-ribosylation factor GTPase-activating protein ASAP1. Evidence for the pleckstrin homology domain functioning as an allosteric site.

Authors:  J L Kam; K Miura; T R Jackson; J Gruschus; P Roller; S Stauffer; J Clark; R Aneja; P A Randazzo
Journal:  J Biol Chem       Date:  2000-03-31       Impact factor: 5.157

7.  Mislocalization or reduced expression of Arf GTPase-activating protein ASAP1 inhibits cell spreading and migration by influencing Arf1 GTPase cycling.

Authors:  Yunhao Liu; Gil M Yerushalmi; Pablo R Grigera; J Thomas Parsons
Journal:  J Biol Chem       Date:  2005-01-04       Impact factor: 5.157

8.  Association of a cellular myosin II with anionic phospholipids and the neuronal plasma membrane.

Authors:  D Li; M Miller; P D Chantler
Journal:  Proc Natl Acad Sci U S A       Date:  1994-02-01       Impact factor: 11.205

9.  Molecular Basis for Cooperative Binding of Anionic Phospholipids to the PH Domain of the Arf GAP ASAP1.

Authors:  Xiaoying Jian; Wai-Kwan Tang; Peng Zhai; Neeladri Sekhar Roy; Ruibai Luo; James M Gruschus; Marielle E Yohe; Pei-Wen Chen; Yifei Li; R Andrew Byrd; Di Xia; Paul A Randazzo
Journal:  Structure       Date:  2015-09-10       Impact factor: 5.006

10.  The association of ASAP1, an ADP ribosylation factor-GTPase activating protein, with focal adhesion kinase contributes to the process of focal adhesion assembly.

Authors:  Yunhao Liu; Joost C Loijens; Karen H Martin; Andrei V Karginov; J Thomas Parsons
Journal:  Mol Biol Cell       Date:  2002-06       Impact factor: 4.138
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  12 in total

Review 1.  Arf GAPs and molecular motors.

Authors:  Ruibai Luo; Christine E Reed; Jeffrey A Sload; Linda Wordeman; Paul A Randazzo; Pei-Wen Chen
Journal:  Small GTPases       Date:  2017-04-21

2.  The BAR domain of the Arf GTPase-activating protein ASAP1 directly binds actin filaments.

Authors:  Pei-Wen Chen; Neil Billington; Ben Y Maron; Jeffrey A Sload; Krishna Chinthalapudi; Sarah M Heissler
Journal:  J Biol Chem       Date:  2020-05-22       Impact factor: 5.157

Review 3.  Arf GAPs: A family of proteins with disparate functions that converge on a common structure, the integrin adhesion complex.

Authors:  Teresa Vitali; Sofia Girald-Berlingeri; Paul A Randazzo; Pei-Wen Chen
Journal:  Small GTPases       Date:  2017-03-31

4.  PEAK3/C19orf35 pseudokinase, a new NFK3 kinase family member, inhibits CrkII through dimerization.

Authors:  Mitchell L Lopez; Megan Lo; Jennifer E Kung; Małgorzata Dudkiewicz; Gwendolyn M Jang; John Von Dollen; Jeffrey R Johnson; Nevan J Krogan; Krzysztof Pawłowski; Natalia Jura
Journal:  Proc Natl Acad Sci U S A       Date:  2019-07-16       Impact factor: 11.205

Review 5.  Mammalian nonmuscle myosin II comes in three flavors.

Authors:  Maria S Shutova; Tatyana M Svitkina
Journal:  Biochem Biophys Res Commun       Date:  2018-03-17       Impact factor: 3.575

6.  ARAP2 inhibits Akt independently of its effects on focal adhesions.

Authors:  Ruibai Luo; Pei-Wen Chen; Jean-Cheng Kuo; Lisa Jenkins; Xiaoying Jian; Clare M Waterman; Paul A Randazzo
Journal:  Biol Cell       Date:  2018-09-10       Impact factor: 4.458

Review 7.  The state of F-BAR domains as membrane-bound oligomeric platforms.

Authors:  Chloe E Snider; Wan Nurul Izzati Wan Mohamad Noor; Nhung Thi Hong Nguyen; Kathleen L Gould; Shiro Suetsugu
Journal:  Trends Cell Biol       Date:  2021-04-20       Impact factor: 21.167

Review 8.  Arf proteins in cancer cell migration.

Authors:  Cristina Casalou; Alexandra Faustino; Duarte C Barral
Journal:  Small GTPases       Date:  2016-09-02

Review 9.  ARF GTPases and their GEFs and GAPs: concepts and challenges.

Authors:  Elizabeth Sztul; Pei-Wen Chen; James E Casanova; Jacqueline Cherfils; Joel B Dacks; David G Lambright; Fang-Jen S Lee; Paul A Randazzo; Lorraine C Santy; Annette Schürmann; Ilka Wilhelmi; Marielle E Yohe; Richard A Kahn
Journal:  Mol Biol Cell       Date:  2019-05-15       Impact factor: 4.138

10.  APOL1 C-Terminal Variants May Trigger Kidney Disease through Interference with APOL3 Control of Actomyosin.

Authors:  Sophie Uzureau; Laurence Lecordier; Pierrick Uzureau; Dorle Hennig; Jonas H Graversen; Fabrice Homblé; Pepe Ekulu Mfutu; Fanny Oliveira Arcolino; Ana Raquel Ramos; Rita M La Rovere; Tomas Luyten; Marjorie Vermeersch; Patricia Tebabi; Marc Dieu; Bart Cuypers; Stijn Deborggraeve; Marion Rabant; Christophe Legendre; Søren K Moestrup; Elena Levtchenko; Geert Bultynck; Christophe Erneux; David Pérez-Morga; Etienne Pays
Journal:  Cell Rep       Date:  2020-03-17       Impact factor: 9.423
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