Thamara E Osinga1, Anouk N A van der Horst-Schrivers2, Martijn van Faassen3, Michiel N Kerstens4, Robin P F Dullaart5, Karel Pacak6, Thera P Links7, Ido P Kema8. 1. Department of Endocrinology (AA31), University of Groningen, University Medical Center Groningen, PO-box 30.001, 9700 RB Groningen, The Netherlands. Electronic address: t.osinga@umcg.nl. 2. Department of Endocrinology (AA31), University of Groningen, University Medical Center Groningen, PO-box 30.001, 9700 RB Groningen, The Netherlands. Electronic address: a.n.a.van.der.horst@umcg.nl. 3. Department of Laboratory Medicine (EA61), University of Groningen, University Medical Center Groningen, PO-box 30.001, 9700 RB Groningen, The Netherlands. Electronic address: h.j.r.van.faassen@umcg.nl. 4. Department of Endocrinology (AA31), University of Groningen, University Medical Center Groningen, PO-box 30.001, 9700 RB Groningen, The Netherlands. Electronic address: m.n.kerstens@umcg.nl. 5. Department of Endocrinology (AA31), University of Groningen, University Medical Center Groningen, PO-box 30.001, 9700 RB Groningen, The Netherlands. Electronic address: r.p.f.dullaart@umcg.nl. 6. Program in Reproductive and Adult Endocrinology, Eunice Kennedy Shriver National Institute of Child Health and Human Development, National Institutes of Health, Building 10, Room 1-3140, 10 Center Drive, Bethesda, MD 20892, USA. Electronic address: karel@mail.nih.gov. 7. Department of Endocrinology (AA31), University of Groningen, University Medical Center Groningen, PO-box 30.001, 9700 RB Groningen, The Netherlands. Electronic address: t.p.links@umcg.nl. 8. Department of Laboratory Medicine (EA61), University of Groningen, University Medical Center Groningen, PO-box 30.001, 9700 RB Groningen, The Netherlands. Electronic address: i.p.kema@umcg.nl.
Abstract
BACKGROUND: Determination of metanephrine (MN), normetanephrine (NMN), and 3-methoxytyramine (3-MT) in saliva may offer potential diagnostic advantages in diagnosing pheochromocytoma. METHODS: In this preliminary study, we determined metanephrine concentrations in saliva of healthy subjects and the relationship with simultaneously measured plasma metanephrines. We also studied the possible influence of pre-analytical conditions such as a collection device, awakening, posture, and eating on the salivary metanephrine levels. RESULTS: Eleven healthy subjects were included. Fasting blood and saliva samples were collected in seated position and after 30min of horizontal rest. Plasma and salivary MN, NMN, and 3-MT concentrations were determined using a high-performance liquid chromatography tandem mass spectrometric technique (LC-MS/MS) with automated solid phase extraction sample preparation. Metanephrines were detectable in saliva from all participants both in seated and supine position. No significant correlations were observed between the MN, NMN, and 3-MT concentrations in saliva and plasma in seated or supine position. Furthermore, there was no difference between MN, NMN, and 3-MT samples collected with or without a collection device. CONCLUSION: Metanephrines can be detected in saliva with LC-MS/MS with sufficient sensitivity and precision. Our findings warrant evaluation of salivary metanephrine measurement as a novel laboratory tool in the work-up of patients suspected of having a pheochromocytoma.
BACKGROUND: Determination of metanephrine (MN), normetanephrine (NMN), and 3-methoxytyramine (3-MT) in saliva may offer potential diagnostic advantages in diagnosing pheochromocytoma. METHODS: In this preliminary study, we determined metanephrine concentrations in saliva of healthy subjects and the relationship with simultaneously measured plasma metanephrines. We also studied the possible influence of pre-analytical conditions such as a collection device, awakening, posture, and eating on the salivary metanephrine levels. RESULTS: Eleven healthy subjects were included. Fasting blood and saliva samples were collected in seated position and after 30min of horizontal rest. Plasma and salivary MN, NMN, and 3-MT concentrations were determined using a high-performance liquid chromatography tandem mass spectrometric technique (LC-MS/MS) with automated solid phase extraction sample preparation. Metanephrines were detectable in saliva from all participants both in seated and supine position. No significant correlations were observed between the MN, NMN, and 3-MT concentrations in saliva and plasma in seated or supine position. Furthermore, there was no difference between MN, NMN, and 3-MT samples collected with or without a collection device. CONCLUSION:Metanephrines can be detected in saliva with LC-MS/MS with sufficient sensitivity and precision. Our findings warrant evaluation of salivary metanephrine measurement as a novel laboratory tool in the work-up of patients suspected of having a pheochromocytoma.
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