| Literature DB >> 26867747 |
Jeffrey R Whiteaker1, Goran N Halusa2, Andrew N Hoofnagle3, Vagisha Sharma4, Brendan MacLean4, Ping Yan1, John A Wrobel5, Jacob Kennedy1, D R Mani6, Lisa J Zimmerman7, Matthew R Meyer8, Mehdi Mesri9, Emily Boja9, Steven A Carr6, Daniel W Chan10, Xian Chen5, Jing Chen10, Sherri R Davies8, Matthew J C Ellis8, David Fenyö11, Tara Hiltke9, Karen A Ketchum12, Chris Kinsinger9, Eric Kuhn6, Daniel C Liebler7, Tao Liu13, Michael Loss2, Michael J MacCoss4, Wei-Jun Qian13, Robert Rivers9, Karin D Rodland13, Kelly V Ruggles11, Mitchell G Scott14, Richard D Smith15, Stefani Thomas10, R Reid Townsend8, Gordon Whiteley2, Chaochao Wu13, Hui Zhang10, Zhen Zhang10, Henry Rodriguez9, Amanda G Paulovich16.
Abstract
The Clinical Proteomic Tumor Analysis Consortium (CPTAC) of the National Cancer Institute (NCI) has launched an Assay Portal (http://assays.cancer.gov) to serve as an open-source repository of well-characterized targeted proteomic assays. The portal is designed to curate and disseminate highly characterized, targeted mass spectrometry (MS)-based assays by providing detailed assay performance characterization data, standard operating procedures, and access to reagents. Assay content is accessed via the portal through queries to find assays targeting proteins associated with specific cellular pathways, protein complexes, or specific chromosomal regions. The position of the peptide analytes for which there are available assays are mapped relative to other features of interest in the protein, such as sequence domains, isoforms, single nucleotide polymorphisms, and posttranslational modifications. The overarching goals are to enable robust quantification of all human proteins and to standardize the quantification of targeted MS-based assays to ultimately enable harmonization of results over time and across laboratories.Entities:
Keywords: Harmonization; MRM; Multiple reaction monitoring; PRM; Quantitative assay database; Quantitative proteomics; SRM; Selected reaction monitoring; Standardization; Targeted mass spectrometry
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Year: 2016 PMID： 26867747 PMCID： PMC5017244 DOI： 10.1007/978-1-4939-3524-6_13
Source DB: PubMed Journal: Methods Mol Biol ISSN： 1064-3745