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Literature DB >> 26855825

Biophysical EPR Studies Applied to Membrane Proteins.

Abstract

Membrane proteins are very important in controlling bioenergetics, functional activity, and initializing signal pathways in a wide variety of complicated biological systems. They also represent approximately 50% of the potential drug targets. EPR spectroscopy is a very popular and powerful biophysical tool that is used to study the structural and dynamic properties of membrane proteins. In this article, a basic overview of the most commonly used EPR techniques and examples of recent applications to answer pertinent structural and dynamic related questions on membrane protein systems will be presented.

Entities: Chemical Disease Gene Species

Keywords: DEER; Electron paramagnetic resonance spectroscopy; Membrane proteins; Site-directed spin labeling; Structural topology and dynamics

Year: 2015 PMID： 26855825 PMCID： PMC4742357 DOI： 10.4172/2161-0398.1000188

Source DB: PubMed Journal: J Phys Chem Biophys

72 in total

Review 1. A new spin on protein dynamics.

Authors: Linda Columbus; Wayne L Hubbell
Journal: Trends Biochem Sci Date: 2002-06 Impact factor: 13.807

2. Localization of the N-terminal domain in light-harvesting chlorophyll a/b protein by EPR measurements.

Authors: Gunnar Jeschke; Alexander Bender; Thorsten Schweikardt; Grazyna Panek; Heinz Decker; Harald Paulsen
Journal: J Biol Chem Date: 2005-03-08 Impact factor: 5.157

3. CHARMM-GUI: a web-based graphical user interface for CHARMM.

Authors: Sunhwan Jo; Taehoon Kim; Vidyashankara G Iyer; Wonpil Im
Journal: J Comput Chem Date: 2008-08 Impact factor: 3.376

4. Site-directed spin labeling demonstrates that transmembrane domain XII in the lactose permease of Escherichia coli is an alpha-helix.

Authors: J Voss; M M He; W L Hubbell; H R Kaback
Journal: Biochemistry Date: 1996-10-01 Impact factor: 3.162

5. Conformational cycle of the ABC transporter MsbA in liposomes: detailed analysis using double electron-electron resonance spectroscopy.

Authors: Ping Zou; Marco Bortolus; Hassane S McHaourab
Journal: J Mol Biol Date: 2009-08-26 Impact factor: 5.469

5. Development of lamellar gel phase emulsion containing baru oil (Dipteryx alata Vog.) as a prospective delivery system for cutaneous application.

Authors: Cristhianne Moraes; Jorge L V Anjos; Monica Maruno; Antonio Alonso; Pedro Rocha-Filho
Journal: Asian J Pharm Sci Date: 2017-10-12 Impact factor: 6.598

Review 6. Site-Directed Spin Labeling EPR for Studying Membrane Proteins.

Authors: Indra D Sahu; Gary A Lorigan
Journal: Biomed Res Int Date: 2018-01-23 Impact factor: 3.411

Review 7. Electron Paramagnetic Resonance as a Tool for Studying Membrane Proteins.

Authors: Indra D Sahu; Gary A Lorigan
Journal: Biomolecules Date: 2020-05-13

7 in total

Biophysical EPR Studies Applied to Membrane Proteins.

Review 1. A new spin on protein dynamics.

2. Localization of the N-terminal domain in light-harvesting chlorophyll a/b protein by EPR measurements.

3. CHARMM-GUI: a web-based graphical user interface for CHARMM.

4. Site-directed spin labeling demonstrates that transmembrane domain XII in the lactose permease of Escherichia coli is an alpha-helix.

5. Conformational cycle of the ABC transporter MsbA in liposomes: detailed analysis using double electron-electron resonance spectroscopy.

Review 6. Use of electron paramagnetic resonance to solve biochemical problems.

7. Structure of KCNE1 and implications for how it modulates the KCNQ1 potassium channel.

Review 8. Long-range distance determinations in biomacromolecules by EPR spectroscopy.

9. A PELDOR-based nanometer distance ruler for oligonucleotides.

10. Structural basis of lipid-driven conformational transitions in the KvAP voltage-sensing domain.

1. Conformational Differences Are Observed for the Active and Inactive Forms of Pinholin S²¹ Using DEER Spectroscopy.

2. Pinholin S²¹ mutations induce structural topology and conformational changes.

Review 3. The two sides of a lipid-protein story.

Review 4. Native Mass Spectrometry of Membrane Proteins.

5. Development of lamellar gel phase emulsion containing baru oil (Dipteryx alata Vog.) as a prospective delivery system for cutaneous application.

Review 6. Site-Directed Spin Labeling EPR for Studying Membrane Proteins.

Review 7. Electron Paramagnetic Resonance as a Tool for Studying Membrane Proteins.